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Literature summary extracted from

  • Luo, Q.; Mao, X.; Kong, L.; Huang, X.; Zou, H.
    High-performance affinity chromatography for characterization of human immunoglobulin G digestion with papain (2002), J. Chromatogr. B, 776, 139-147.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.22.2 free papain loses 60% of its activity after 2 h at 75°C, papain immobilized on the poly(glycidyl methacrylate-co-ethylene dimethylacrylate) monolith loses 10% of its activity Carica papaya
3.4.22.2 the operational stability, expressed as the catalytic half-life reaches about 1 week for the enzyme immobilized on the poly(glycidyl methacrylate-co-ethylene dimethylacrylate) monolith under optimal pH and temperature conditions Carica papaya

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.2 additional information
-
 additional information KM value for human IgG with the immobilized enzyme is 0.55 mg/ml, the Km-value for the free enzyme is 1.08 mg/ml Carica papaya

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.2 Carica papaya
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.2 human IgG + H2O
-
 Carica papaya fragment Fab + fragment Fc
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.2 75
-
 2 h, free papain loses 60% of its activity, papain immobilized on the poly(glycidyl methacrylate-co-ethylene dimethylacrylate) monolith loses 10% of its activity Carica papaya