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Literature summary extracted from

  • Omura, H.; Kuroda, M.; Kobayashi, M.; Shimizu, S.; Yoshida, T.; Nagasawa, T.
    Purification, characterization and gene cloning of thermostable O-acetyl-L-serine sulfhydrylase forming b-cyano-L-alanine (2003), J. Biosci. Bioeng., 95, 470-475.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.9 expression in Escherichia coli Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.47 0.25
-
 L-cysteine pH 7.5, 45°C Geobacillus stearothermophilus
2.5.1.47 16
-
 O3-acetyl-L-serine pH 7.5, 45°C Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.47 34000
-
 2 * 34000, SDS-PAGE Geobacillus stearothermophilus
2.5.1.47 70000
-
 gel filtration Geobacillus stearothermophilus
4.4.1.9 34000
-
 2 * 34000, SDS-PAGE Geobacillus stearothermophilus
4.4.1.9 70000
-
 gel filtration Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.9 L-cysteine + HCN Geobacillus stearothermophilus
-
 sulfide + L-3-cyanoalanine
-
 ?
4.4.1.9 O-acetyl-L-serine + CN- Geobacillus stearothermophilus
-
 acetate + L-3-cyanoalanine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Geobacillus stearothermophilus Q84IF9
-
-
4.4.1.9 Geobacillus stearothermophilus Q84IF9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.47 ethanol precipitation, ammonium sulfate precipitation, preparative electrophoresis, phenyl sepharose Geobacillus stearothermophilus
4.4.1.9 ammonium sulfate, ethanol, heat, DEAE-Cellulofine, gel filtration, Phenyl-Sepharose Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.9 483
-
-
 Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.47 L-cysteine + cyanide
-
 Geobacillus stearothermophilus cyanoalanine + H2S
-
 ?
2.5.1.47 additional information no substrate: L-serine, L-homoserine, O-acetyl-L-homoserine, L-alanine, L-homocysteine Geobacillus stearothermophilus ?
-
 ?
2.5.1.47 O3-acetyl-L-serine + benzylmercaptan
-
 Geobacillus stearothermophilus S-benzyl-L-cysteine + acetate
-
 ?
2.5.1.47 O3-acetyl-L-serine + cyanide
-
 Geobacillus stearothermophilus beta-cyano-L-alanine + acetate
-
 ?
2.5.1.47 O3-acetyl-L-serine + ethylmercaptan
-
 Geobacillus stearothermophilus S-ethyl-L-cysteine + acetate
-
 ?
2.5.1.47 O3-acetyl-L-serine + hydrogen sulfide 970% of the activity with cyanide Geobacillus stearothermophilus L-cysteine + acetate
-
 ?
2.5.1.47 O3-acetyl-L-serine + methylmercaptan
-
 Geobacillus stearothermophilus S-methyl-L-cysteine + acetate
-
 ?
2.5.1.47 O3-acetyl-L-serine + phenol
-
 Geobacillus stearothermophilus O-phenyl-L-serine + acetate
-
 ?
2.5.1.47 O3-acetyl-L-serine + phenylmercaptan
-
 Geobacillus stearothermophilus S-phenyl-L-cysteine + acetate
-
 ?
2.5.1.47 O3-acetyl-L-serine + propylmercaptan
-
 Geobacillus stearothermophilus S-propyl-L-cysteine + acetate
-
 ?
4.4.1.9 L-cysteine + HCN
-
 Geobacillus stearothermophilus sulfide + L-3-cyanoalanine
-
 ?
4.4.1.9 O-acetyl-L-serine + benzylmercaptan
-
 Geobacillus stearothermophilus S-benzyl-L-cysteine + acetate
-
 ?
4.4.1.9 O-acetyl-L-serine + CN-
-
 Geobacillus stearothermophilus acetate + L-3-cyanoalanine
-
 ?
4.4.1.9 O-acetyl-L-serine + ethylmercaptan
-
 Geobacillus stearothermophilus S-ethyl-L-cysteine + acetate
-
 ?
4.4.1.9 O-acetyl-L-serine + methylmercaptan
-
 Geobacillus stearothermophilus S-methyl-L-cysteine + acetate
-
 ?
4.4.1.9 O-acetyl-L-serine + phenol
-
 Geobacillus stearothermophilus S-phenyl-L-serine + acetate
-
 ?
4.4.1.9 O-acetyl-L-serine + phenylmercaptan
-
 Geobacillus stearothermophilus S-phenyl-L-cysteine + acetate
-
 ?
4.4.1.9 O-acetyl-L-serine + propylmercaptan
-
 Geobacillus stearothermophilus S-propyl-L-cysteine + acetate
-
 ?
4.4.1.9 O-acetyl-L-serine + sulfide 970% of activity with potassium cyanide Geobacillus stearothermophilus L-cysteine + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.47 dimer 2 * 34000, SDS-PAGE Geobacillus stearothermophilus
4.4.1.9 dimer 2 * 34000, SDS-PAGE Geobacillus stearothermophilus

Synonyms

EC Number Synonyms Comment Organism
2.5.1.47 beta-cyano-L-alanine synthase
-
 Geobacillus stearothermophilus
4.4.1.9 O-acetyl-L-serine sulfhydrylase
-
 Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.47 45
-
-
 Geobacillus stearothermophilus
4.4.1.9 45
-
-
 Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.47 60
-
 30 min, stable in pH-range of 6.0-10.0 Geobacillus stearothermophilus
2.5.1.47 70
-
 30 min, pH 7.5, stable Geobacillus stearothermophilus
4.4.1.9 60
-
 stable for 30 min at pH 6.0-10.0 Geobacillus stearothermophilus
4.4.1.9 70
-
 no loss of activity after 30 min at pH 7.5 Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.47 8
-
-
 Geobacillus stearothermophilus
4.4.1.9 8
-
-
 Geobacillus stearothermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.4.1.9 4.5 10.5 approx. 50% of maximal activity at pH 4.5 and pH 10.5, respectively Geobacillus stearothermophilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.5.1.47 6 10
-
 Geobacillus stearothermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.9 pyridoxal 5'-phosphate
-
 Geobacillus stearothermophilus