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Literature summary extracted from

  • Saegusa, H.; Shiraki, M.; Saito, T.
    Cloning of an intracellular D(-)-3-hydroxybutyrate-oligomer hydrolase gene from Ralstonia eutropha H16 and identification of the active site serine residue by site-directed mutagenesis (2002), J. Biosci. Bioeng., 94, 106-112.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.22 DNA sequence determination and analysis, functional expression in Escherichia coli as His-tagged enzyme, in vitro transcription and translation Cupriavidus necator

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.22 S191A site-directed mutagenesis, unaltered activity compared to the wild-type enzyme Cupriavidus necator
3.1.1.22 S218A site-directed mutagenesis, slightly decreased activity compared to the wild-type enzyme Cupriavidus necator
3.1.1.22 S306A site-directed mutagenesis, slightly increased activity compared to the wild-type enzyme Cupriavidus necator
3.1.1.22 S356A site-directed mutagenesis, slightly increased activity compared to the wild-type enzyme Cupriavidus necator
3.1.1.22 S359A site-directed mutagenesis, inactive mutant Cupriavidus necator
3.1.1.22 S630A site-directed mutagenesis, increased activity compared to the wild-type enzyme Cupriavidus necator

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.22 intracellular
-
 Cupriavidus necator 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.22 78000
-
 x * 78000, invitro transcribed and translated enzyme, SDS-PAGE Cupriavidus necator

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.22 Cupriavidus necator Q0K9H3 formerly Alcaligenes eutrophus, strain H16, ATCC 17699
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.22 recombinant from Escherichia coli Cupriavidus necator

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.1.22 (R)-3-((R)-3-hydroxybutanoyloxy)butanoate + H2O = 2 (R)-3-hydroxybutanoate Ser359 is invlved in the catalytic mechanism, active site residue Cupriavidus necator
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.22 (R)-3-((R)-3-hydroxybutanoyloxy)2butanoate + H2O
-
 Cupriavidus necator (R)-3-hydroxybutanoate
-
 ?
3.1.1.22 (R)-3-((R)-3-hydroxybutanoyloxy)butanoate + H2O
-
 Cupriavidus necator (R)-3-hydroxybutanoate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.22 ? x * 78000, invitro transcribed and translated enzyme, SDS-PAGE Cupriavidus necator

Synonyms

EC Number Synonyms Comment Organism
3.1.1.22 3HB oligomer hydrolase
-
 Cupriavidus necator
3.1.1.22 D-(-)-3-hydroxybutyrate oligomer hydrolase
-
 Cupriavidus necator

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.22 30
-
 assay at Cupriavidus necator

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.22 8
-
 assay at Cupriavidus necator