BRENDA - Enzyme Database show

Investigations on the metal switch region of human porphobilinogen synthase

Jaffe, E.K.; J. Biol. Inorg. Chem. 8, 176-184 (2003)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
4.2.1.24
additional information
chimeric proteins that contain the aspartate-rich sequences of the pea enzyme or the enzyme from Pseudomonas aeruginosa in place of the naturally occuring cysteine-rich sequence of the human enzyme. The chimeric enzymes are substantially activated by both magnesium and potassium, but not by zinc. The specific activities of the chimeras are significantly lower than the specific activity of the wild-type enzyme
Homo sapiens
4.2.1.24
R221K
mutation in wild-type or chimeric enzymes reduces activity
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.24
Mg2+
chimeric proteins are constructed that contain the aspartate-rich sequences of the pea enzyme or the enzyme from Pseudomonas aeruginosa in place of the naturally occuring cysteine-rich sequence of the human enzyme. The chimeric enzymes are substantially activated by both magnesium and potassium, but not by zinc
Homo sapiens
4.2.1.24
Zn2+
the cysteines of the metal switch sequence of the wild-type enzyme bind a catalytic zinc. Chimeric proteins are constructed that contain the aspartate-rich sequences of the pea enzyme or the enzyme from Pseudomonas aeruginosa in place of the naturally occuring cysteine-rich sequence of the human enzyme. The chimeric enzymes are substantially activated by both magnesium and potassium, but not by zinc.
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
Homo sapiens
the enzyme functions in the first common step in tetrapyrrole biosynthesis
porphobilinogen + 2 H2O
-
Homo sapiens
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.24
Homo sapiens
P13716
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
the enzyme functions in the first common step in tetrapyrrole biosynthesis
652574
Homo sapiens
porphobilinogen + 2 H2O
-
652574
Homo sapiens
?
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
4.2.1.24
additional information
chimeric proteins that contain the aspartate-rich sequences of the pea enzyme or the enzyme from Pseudomonas aeruginosa in place of the naturally occuring cysteine-rich sequence of the human enzyme. The chimeric enzymes are substantially activated by both magnesium and potassium, but not by zinc. The specific activities of the chimeras are significantly lower than the specific activity of the wild-type enzyme
Homo sapiens
4.2.1.24
R221K
mutation in wild-type or chimeric enzymes reduces activity
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.24
Mg2+
chimeric proteins are constructed that contain the aspartate-rich sequences of the pea enzyme or the enzyme from Pseudomonas aeruginosa in place of the naturally occuring cysteine-rich sequence of the human enzyme. The chimeric enzymes are substantially activated by both magnesium and potassium, but not by zinc
Homo sapiens
4.2.1.24
Zn2+
the cysteines of the metal switch sequence of the wild-type enzyme bind a catalytic zinc. Chimeric proteins are constructed that contain the aspartate-rich sequences of the pea enzyme or the enzyme from Pseudomonas aeruginosa in place of the naturally occuring cysteine-rich sequence of the human enzyme. The chimeric enzymes are substantially activated by both magnesium and potassium, but not by zinc.
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
Homo sapiens
the enzyme functions in the first common step in tetrapyrrole biosynthesis
porphobilinogen + 2 H2O
-
Homo sapiens
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
the enzyme functions in the first common step in tetrapyrrole biosynthesis
652574
Homo sapiens
porphobilinogen + 2 H2O
-
652574
Homo sapiens
?