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Literature summary extracted from

  • Morty, R.E.; Morehead, J.
    Cloning and characterization of a leucyl aminopeptidase from three pathogenic Leishmania species (2002), J. Biol. Chem., 29, 26057-26065.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.1 constitutive single copy lap gene, DNA and amino acid sequence determination and analysis, expression in Escherichia coli as His-tagged protein Leishmania donovani
3.4.11.1 constitutive single copy lap gene, DNA and amino acid sequence determination and analysis, expression in Escherichia coli as His-tagged protein Leishmania major
3.4.11.1 constitutive single copy lap gene, DNA and amino acid sequence determination and analysis, expression in Escherichia coli as His-tagged protein Leishmania amazonensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.1 1,10-phenanthroline strong inhibition Leishmania amazonensis
3.4.11.1 1,10-phenanthroline strong inhibition Leishmania donovani
3.4.11.1 1,10-phenanthroline strong inhibition Leishmania major
3.4.11.1 1,7-phenanthroline slight inhibition Leishmania amazonensis
3.4.11.1 1,7-phenanthroline slight inhibition Leishmania donovani
3.4.11.1 1,7-phenanthroline slight inhibition Leishmania major
3.4.11.1 actinonin tight-binding, competitive inhibitor Leishmania amazonensis
3.4.11.1 actinonin tight-binding, competitive inhibitor Leishmania donovani
3.4.11.1 actinonin tight-binding, competitive inhibitor Leishmania major
3.4.11.1 apstatin strong inhibition Leishmania amazonensis
3.4.11.1 apstatin strong inhibition Leishmania donovani
3.4.11.1 apstatin strong inhibition Leishmania major
3.4.11.1 arphamenine A non-tight-binding, competitive inhibitor Leishmania amazonensis
3.4.11.1 arphamenine A non-tight-binding, competitive inhibitor Leishmania donovani
3.4.11.1 arphamenine A non-tight-binding, competitive inhibitor Leishmania major
3.4.11.1 bestatin strong inhibition Leishmania amazonensis
3.4.11.1 bestatin strong inhibition Leishmania donovani
3.4.11.1 bestatin strong inhibition Leishmania major
3.4.11.1 Ca2+ complete inhibition at 0.1-10 mM Leishmania amazonensis
3.4.11.1 Cu2+ 93% inhibition at 1 mM Leishmania amazonensis
3.4.11.1 DL-thiorphan slight inhibition at 0.1 mM Leishmania amazonensis
3.4.11.1 DL-thiorphan slight inhibition at 0.1 mM Leishmania donovani
3.4.11.1 DL-thiorphan slight inhibition at 0.1 mM Leishmania major
3.4.11.1 EDTA strong inhibition Leishmania amazonensis
3.4.11.1 EDTA strong inhibition Leishmania donovani
3.4.11.1 EDTA strong inhibition Leishmania major
3.4.11.1 EGTA
-
Leishmania amazonensis
3.4.11.1 EGTA
-
Leishmania donovani
3.4.11.1 EGTA
-
Leishmania major
3.4.11.1 Fe2+ complete inhibition at 1-10 mM Leishmania amazonensis
3.4.11.1 GSH slight inhibition Leishmania donovani
3.4.11.1 GSH slight inhibition Leishmania major
3.4.11.1 L-Leucinol non-tight-binding, competitive inhibitor Leishmania amazonensis
3.4.11.1 L-Leucinol non-tight-binding, competitive inhibitor Leishmania donovani
3.4.11.1 L-Leucinol non-tight-binding, competitive inhibitor Leishmania major
3.4.11.1 additional information no inhibition by DTT and GSH, inhibition mechanisms Leishmania amazonensis
3.4.11.1 additional information no inhibition by DTT, inhibition mechanisms Leishmania donovani
3.4.11.1 additional information no inhibition by DTT, inhibition mechanisms Leishmania major
3.4.11.1 phosphoramidon slight inhibition at 0.1 mM Leishmania amazonensis
3.4.11.1 phosphoramidon slight inhibition at 0.1 mM Leishmania donovani
3.4.11.1 phosphoramidon slight inhibition at 0.1 mM Leishmania major
3.4.11.1 puromycin slight inhibition Leishmania amazonensis
3.4.11.1 puromycin slight inhibition Leishmania donovani
3.4.11.1 puromycin slight inhibition Leishmania major
3.4.11.1 Zn2+ inhibitory at 10 mM, slightly activating at 1 mM Leishmania amazonensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.1 0.043
-
L-Cys-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania amazonensis
3.4.11.1 0.044
-
L-Cys-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania major
3.4.11.1 0.054
-
L-Ile-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania major
3.4.11.1 0.06
-
L-Ile-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania amazonensis
3.4.11.1 0.062
-
L-Cys-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania donovani
3.4.11.1 0.072
-
L-Trp-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania amazonensis
3.4.11.1 0.077
-
L-Ile-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania donovani
3.4.11.1 0.104
-
L-Trp-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania donovani
3.4.11.1 0.105
-
L-Met-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania amazonensis
3.4.11.1 0.118
-
L-Trp-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania major
3.4.11.1 0.137
-
L-Met-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania donovani
3.4.11.1 0.154
-
L-Met-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania major
3.4.11.1 0.167
-
L-Leu-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania major
3.4.11.1 0.189
-
L-Leu-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania amazonensis
3.4.11.1 0.229
-
L-Leu-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania donovani
3.4.11.1 0.392
-
L-Ala-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania major
3.4.11.1 0.408
-
L-Ala-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania donovani
3.4.11.1 0.442
-
L-Ala-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania amazonensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.1 cytosol
-
Leishmania amazonensis 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.1 Co2+ activates best at 1 mM Leishmania amazonensis
3.4.11.1 Mg2+ activates at 1-10 mM Leishmania amazonensis
3.4.11.1 Mn2+ activates at 0.1-1 mM Leishmania amazonensis
3.4.11.1 Ni2+ activates at 1-10 mM Leishmania amazonensis
3.4.11.1 Zn2+ bound to the enzyme as native cofactor Leishmania amazonensis
3.4.11.1 Zn2+ inhibitory at 10 mM, slightly activating at 1 mM Leishmania amazonensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.1 62337
-
6 * 62337, recombinant His-tagged enzyme, amino acid sequence calculation Leishmania donovani
3.4.11.1 62424
-
6 * 62424, recombinant His-tagged enzyme, amino acid sequence calculation Leishmania amazonensis
3.4.11.1 62569
-
6 * 62569, recombinant His-tagged enzyme, amino acid sequence calculation Leishmania major
3.4.11.1 360000
-
gel filtration Leishmania amazonensis

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.1 Leishmania amazonensis Q95V76
-
-
3.4.11.1 Leishmania donovani Q95V75
-
-
3.4.11.1 Leishmania major Q7KF27
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.1 additional information
-
-
Leishmania donovani
3.4.11.1 additional information
-
-
Leishmania major
3.4.11.1 additional information
-
-
Leishmania amazonensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.1 L-Ala-7-amido-4-methylcoumarin + H2O low activity Leishmania donovani L-Ala + 7-amino-4-methylcoumarin
-
?
3.4.11.1 L-Ala-7-amido-4-methylcoumarin + H2O low activity Leishmania major L-Ala + 7-amino-4-methylcoumarin
-
?
3.4.11.1 L-Ala-7-amido-4-methylcoumarin + H2O low activity Leishmania amazonensis L-Ala + 7-amino-4-methylcoumarin
-
?
3.4.11.1 L-Cys-7-amido-4-methylcoumarin + H2O
-
Leishmania donovani L-Cys + 7-amino-4-methylcoumarin
-
?
3.4.11.1 L-Cys-7-amido-4-methylcoumarin + H2O
-
Leishmania major L-Cys + 7-amino-4-methylcoumarin
-
?
3.4.11.1 L-Cys-7-amido-4-methylcoumarin + H2O
-
Leishmania amazonensis L-Cys + 7-amino-4-methylcoumarin
-
?
3.4.11.1 L-Ile-7-amido-4-methylcoumarin + H2O low activity Leishmania donovani L-Ile + 7-amino-4-methylcoumarin
-
?
3.4.11.1 L-Ile-7-amido-4-methylcoumarin + H2O low activity Leishmania major L-Ile + 7-amino-4-methylcoumarin
-
?
3.4.11.1 L-Ile-7-amido-4-methylcoumarin + H2O low activity Leishmania amazonensis L-Ile + 7-amino-4-methylcoumarin
-
?
3.4.11.1 L-Leu-7-amido-4-methylcoumarin + H2O best synthetic substrate Leishmania donovani L-Leu + 7-amino-4-methylcoumarin
-
?
3.4.11.1 L-Leu-7-amido-4-methylcoumarin + H2O best synthetic substrate Leishmania major L-Leu + 7-amino-4-methylcoumarin
-
?
3.4.11.1 L-Leu-7-amido-4-methylcoumarin + H2O best synthetic substrate Leishmania amazonensis L-Leu + 7-amino-4-methylcoumarin
-
?
3.4.11.1 L-Met-7-amido-4-methylcoumarin + H2O
-
Leishmania donovani L-Met + 7-amino-4-methylcoumarin
-
?
3.4.11.1 L-Met-7-amido-4-methylcoumarin + H2O
-
Leishmania major L-Met + 7-amino-4-methylcoumarin
-
?
3.4.11.1 L-Met-7-amido-4-methylcoumarin + H2O
-
Leishmania amazonensis L-Met + 7-amino-4-methylcoumarin
-
?
3.4.11.1 L-Trp-7-amido-4-methylcoumarin + H2O low activity Leishmania donovani L-Trp + 7-amino-4-methylcoumarin
-
?
3.4.11.1 L-Trp-7-amido-4-methylcoumarin + H2O low activity Leishmania major L-Trp + 7-amino-4-methylcoumarin
-
?
3.4.11.1 L-Trp-7-amido-4-methylcoumarin + H2O low activity Leishmania amazonensis L-Trp + 7-amino-4-methylcoumarin
-
?
3.4.11.1 additional information no activity with any other L-amino acid amide, N-carboxybenzyloxy-L-Leu-beta-naphthylamide, and N-carboxybenzyloxy-Gly-Gly-Leu-4-nitroanilide Leishmania donovani ?
-
?
3.4.11.1 additional information no activity with any other L-amino acid amide, N-carboxybenzyloxy-L-Leu-beta-naphthylamide, and N-carboxybenzyloxy-Gly-Gly-Leu-4-nitroanilide Leishmania major ?
-
?
3.4.11.1 additional information no activity with any other L-amino acid amide, N-carboxybenzyloxy-L-Leu-beta-naphthylamide, and N-carboxybenzyloxy-Gly-Gly-Leu-4-nitroanilide Leishmania amazonensis ?
-
?

Subunits

EC Number Subunits Comment Organism
3.4.11.1 hexamer 6 * 62337, recombinant His-tagged enzyme, amino acid sequence calculation Leishmania donovani
3.4.11.1 hexamer 6 * 62424, recombinant His-tagged enzyme, amino acid sequence calculation Leishmania amazonensis
3.4.11.1 hexamer 6 * 62569, recombinant His-tagged enzyme, amino acid sequence calculation Leishmania major

Synonyms

EC Number Synonyms Comment Organism
3.4.11.1 LAP
-
Leishmania donovani
3.4.11.1 LAP
-
Leishmania major
3.4.11.1 LAP
-
Leishmania amazonensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.1 37
-
assay at Leishmania donovani
3.4.11.1 37
-
assay at Leishmania major
3.4.11.1 37
-
assay at Leishmania amazonensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.1 additional information
-
L-Met-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania major
3.4.11.1 0.057 0.65 L-Leu-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania major
3.4.11.1 0.07
-
L-Trp-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania amazonensis
3.4.11.1 0.09
-
L-Ile-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania donovani
3.4.11.1 0.19
-
L-Ile-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania major
3.4.11.1 0.19
-
L-Ile-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania amazonensis
3.4.11.1 0.21
-
L-Trp-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania donovani
3.4.11.1 0.22
-
L-Trp-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania major
3.4.11.1 0.41
-
L-Leu-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania amazonensis
3.4.11.1 0.52
-
L-Leu-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania donovani
3.4.11.1 1.61
-
L-Ala-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania amazonensis
3.4.11.1 1.89
-
L-Ala-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania major
3.4.11.1 2.01
-
L-Ala-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania donovani
3.4.11.1 2.19
-
L-Cys-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania major
3.4.11.1 2.72
-
L-Cys-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania amazonensis
3.4.11.1 2.94
-
L-Ala-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania donovani
3.4.11.1 2.94
-
L-Ala-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania major
3.4.11.1 2.94
-
L-Ala-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania amazonensis
3.4.11.1 2.94
-
L-Cys-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania major
3.4.11.1 3 6 L-Cys-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania donovani
3.4.11.1 3 6 L-Cys-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania amazonensis
3.4.11.1 3.3
-
L-Met-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania donovani
3.4.11.1 3.3
-
L-Met-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania amazonensis
3.4.11.1 3.34
-
L-Cys-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania donovani
3.4.11.1 4.99
-
L-Met-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania amazonensis
3.4.11.1 5.08
-
L-Met-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania donovani
3.4.11.1 5.52
-
L-Met-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania major

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.1 8
-
assay at Leishmania donovani
3.4.11.1 8
-
assay at Leishmania major
3.4.11.1 8.5
-
-
Leishmania amazonensis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.11.1 0.0000008
-
actinonin
-
Leishmania donovani
3.4.11.1 0.000001
-
actinonin
-
Leishmania amazonensis
3.4.11.1 0.0000029
-
actinonin
-
Leishmania major
3.4.11.1 0.000003
-
bestatin
-
Leishmania amazonensis
3.4.11.1 0.000044
-
apstatin
-
Leishmania amazonensis
3.4.11.1 0.059
-
arphamenine A
-
Leishmania donovani
3.4.11.1 0.069
-
arphamenine A
-
Leishmania major
3.4.11.1 0.07
-
arphamenine A
-
Leishmania amazonensis
3.4.11.1 0.102
-
L-Leucinol
-
Leishmania major
3.4.11.1 0.109
-
L-Leucinol
-
Leishmania amazonensis
3.4.11.1 0.118
-
L-Leucinol
-
Leishmania donovani