BRENDA - Enzyme Database show

delta-Aminolevulinic acid dehydratase from Plasmodium falciparum: indigenous versus imported

Dhanasekaran, S.; Chandra, N.R.; Chandrasekhar Sagar, B.K.; Rangarajan, P.N.; Padmanaban, G.; J. Biol. Chem. 279, 6934-6942 (2004)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.24
very poor expression of full-length cDNA, overexpression of cDNA-2 (56 to 451 aa) as a protein with a histidine tag or as a GST fusion protein in Escherichia coli
Plasmodium falciparum
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.24
EDTA
5 mM, 90% inhibition
Mus musculus
4.2.1.24
additional information
no inhibition by EDTA even at 25 mM
Plasmodium falciparum
4.2.1.24
succinylacetone
50% inhibition by 125 nM
Mus musculus
4.2.1.24
succinylacetone
50% inhibition by 250 nM
Plasmodium falciparum
4.2.1.24
Zn2+
pH 7.5, 50% inhibition at 0.12 mM
Mus musculus
4.2.1.24
Zn2+
at pH 8.5, 50% inhibition by 0.075 mM. At pH 7.5, 50% inhibition by less than 0.02 mM
Plasmodium falciparum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.24
0.208
-
5-aminolevulinate
pH 8.5
Plasmodium falciparum
4.2.1.24
0.294
-
5-aminolevulinate
pH 7.5
Mus musculus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.24
apicoplast
-
Mus musculus
20011
-
4.2.1.24
apicoplast
-
Plasmodium falciparum
20011
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.24
K+
stimulates, between pH 6.5 and 7.0, K+ is as stimulatory as Mg2+ and the stimulation is almost 2fold
Plasmodium falciparum
4.2.1.24
Mg2+
20-30% stimulation at pH 8.5, no requirement for a metal ion
Plasmodium falciparum
4.2.1.24
additional information
no stimulation by Mg2+
Mus musculus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.24
38000
-
8 * 38000, mature recombinant enzyme, SDS-PAGE
Mus musculus
4.2.1.24
46000
-
8 * 46000, mature recombinant enzyme, SDS-PAGE
Plasmodium falciparum
4.2.1.24
335000
-
gel filtration
Mus musculus
4.2.1.24
345000
-
gel filtration
Plasmodium falciparum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
Plasmodium falciparum
the role of the enzyme may be confined to heme synthesis in the apicoplast that may not account for the total de novo heme biosynthesis in the parasite
porphobilinogen + 2 H2O
-
Plasmodium falciparum
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.24
Mus musculus
-
-
-
4.2.1.24
Plasmodium falciparum
-
the Plasmodium falciparum enzyme may account for about 10% of the total delta-aminolevulinate dehydratase activity in the parasite, the rest being accounted for by the host enzyme imported by the parasite
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
-
652563
Mus musculus
porphobilinogen + 2 H2O
-
652563
Mus musculus
?
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
-
652563
Plasmodium falciparum
porphobilinogen + 2 H2O
-
652563
Plasmodium falciparum
?
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
the role of the enzyme may be confined to heme synthesis in the apicoplast that may not account for the total de novo heme biosynthesis in the parasite
652563
Plasmodium falciparum
porphobilinogen + 2 H2O
-
652563
Plasmodium falciparum
?
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.24
octamer
8 * 38000, mature recombinant enzyme, SDS-PAGE
Mus musculus
4.2.1.24
octamer
8 * 46000, mature recombinant enzyme, SDS-PAGE
Plasmodium falciparum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.24
7.5
8.5
-
Mus musculus
4.2.1.24
8
9
-
Plasmodium falciparum
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.2.1.24
6.5
9.5
pH 6.5: about 80% of maximal activity, pH 9.5: about 45% of maximal activity
Mus musculus
4.2.1.24
7
10
pH 7.0: about 50% of maximal activity, pH 10.0: about 55% of maximal activity
Plasmodium falciparum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.24
very poor expression of full-length cDNA, overexpression of cDNA-2 (56 to 451 aa) as a protein with a histidine tag or as a GST fusion protein in Escherichia coli
Plasmodium falciparum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.24
EDTA
5 mM, 90% inhibition
Mus musculus
4.2.1.24
additional information
no inhibition by EDTA even at 25 mM
Plasmodium falciparum
4.2.1.24
succinylacetone
50% inhibition by 125 nM
Mus musculus
4.2.1.24
succinylacetone
50% inhibition by 250 nM
Plasmodium falciparum
4.2.1.24
Zn2+
pH 7.5, 50% inhibition at 0.12 mM
Mus musculus
4.2.1.24
Zn2+
at pH 8.5, 50% inhibition by 0.075 mM. At pH 7.5, 50% inhibition by less than 0.02 mM
Plasmodium falciparum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.24
0.208
-
5-aminolevulinate
pH 8.5
Plasmodium falciparum
4.2.1.24
0.294
-
5-aminolevulinate
pH 7.5
Mus musculus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.24
apicoplast
-
Mus musculus
20011
-
4.2.1.24
apicoplast
-
Plasmodium falciparum
20011
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.24
K+
stimulates, between pH 6.5 and 7.0, K+ is as stimulatory as Mg2+ and the stimulation is almost 2fold
Plasmodium falciparum
4.2.1.24
Mg2+
20-30% stimulation at pH 8.5, no requirement for a metal ion
Plasmodium falciparum
4.2.1.24
additional information
no stimulation by Mg2+
Mus musculus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.24
38000
-
8 * 38000, mature recombinant enzyme, SDS-PAGE
Mus musculus
4.2.1.24
46000
-
8 * 46000, mature recombinant enzyme, SDS-PAGE
Plasmodium falciparum
4.2.1.24
335000
-
gel filtration
Mus musculus
4.2.1.24
345000
-
gel filtration
Plasmodium falciparum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
Plasmodium falciparum
the role of the enzyme may be confined to heme synthesis in the apicoplast that may not account for the total de novo heme biosynthesis in the parasite
porphobilinogen + 2 H2O
-
Plasmodium falciparum
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
-
652563
Mus musculus
porphobilinogen + 2 H2O
-
652563
Mus musculus
?
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
-
652563
Plasmodium falciparum
porphobilinogen + 2 H2O
-
652563
Plasmodium falciparum
?
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
the role of the enzyme may be confined to heme synthesis in the apicoplast that may not account for the total de novo heme biosynthesis in the parasite
652563
Plasmodium falciparum
porphobilinogen + 2 H2O
-
652563
Plasmodium falciparum
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.24
octamer
8 * 38000, mature recombinant enzyme, SDS-PAGE
Mus musculus
4.2.1.24
octamer
8 * 46000, mature recombinant enzyme, SDS-PAGE
Plasmodium falciparum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.24
7.5
8.5
-
Mus musculus
4.2.1.24
8
9
-
Plasmodium falciparum
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.2.1.24
6.5
9.5
pH 6.5: about 80% of maximal activity, pH 9.5: about 45% of maximal activity
Mus musculus
4.2.1.24
7
10
pH 7.0: about 50% of maximal activity, pH 10.0: about 55% of maximal activity
Plasmodium falciparum