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Literature summary extracted from

  • Ahuja, A.; Purcarea, C.; Ebert, R.; Sadecki, S.; Guy, H.I.; Evans, D.R.
    Aquifex aeolicus dihydroorotase: association with aspartate transcarbamoylase switches on catalytic activity (2004), J. Biol. Chem., 279, 53136-53144.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.3 expression in Escherichia coli Aquifex aeolicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.3 3.03
-
 dihydroorotate
-
 Aquifex aeolicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.3 Zn2+ one mol per mol of protein Aquifex aeolicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.3 49000
-
 1 * 49000, SDS-PAGE Aquifex aeolicus
3.5.2.3 53000
-
 gel filtration Aquifex aeolicus
3.5.2.3 386000
-
 gel filtration, enzyme-aspartate transcarbamoylase complex Aquifex aeolicus

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.3 Aquifex aeolicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.3 His-tagged recombinant protein Aquifex aeolicus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.2.3 (S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate complex formation of enzyme with aspartate transcarbamoylase drives reaction in the biosynthetic direction Aquifex aeolicus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.3 dihydroorotate + H2O
-
 Aquifex aeolicus N-carbamoyl-L-aspartate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.2.3 monomer 1 * 49000, SDS-PAGE Aquifex aeolicus
3.5.2.3 More recombinant protein lacks catalytic activity, activity is acquired by forming a complex with aspartate transcarbamoylase, complex may be a heterohexamer or dodecamer Aquifex aeolicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.2.3 99
-
 enzyme complex with aspartate transcarbamoylase, both activities are stable Aquifex aeolicus