BRENDA - Enzyme Database

Divergence in non-cognate amino acid recognition between class I and class II lysyl-tRNA synthetases

Levengood, J.D.; Ataide, S.F.; Roy, H.; Ibba, M.; J. Biol. Chem. 279, 17707-17714 (2004)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
6.1.1.6
expression in strain BL21(DE3)
Escherichia coli
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
6.1.1.6
5'-O-[N-(L-lysyl)sulfamoyl]adenosine
-
Borreliella burgdorferi
6.1.1.6
5'-O-[N-(L-lysyl)sulfamoyl]adenosine
-
Escherichia coli
6.1.1.6
AEC
competitive versus L-lysine
Escherichia coli
6.1.1.6
cadaverine
-
Escherichia coli
6.1.1.6
D-Lysine
competitive versus L-lysine
Escherichia coli
6.1.1.6
gamma-aminobutyric acid
-
Borreliella burgdorferi
6.1.1.6
L-arginine
-
Borreliella burgdorferi
6.1.1.6
lysinamide
competitive versus L-lysine
Escherichia coli
6.1.1.6
lysine ethyl ester
competitive versus L-lysine
Escherichia coli
6.1.1.6
lysine methyl ester
competitive versus L-lysine
Escherichia coli
6.1.1.6
additional information
no inhibition by lysinamide in vivo
Borreliella burgdorferi
6.1.1.6
ornithine
competitive versus L-lysine
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.1.1.6
ATP + L-lysine + tRNALys
Escherichia coli
-
AMP + L-lysyl-tRNALys + diphosphate
-
Escherichia coli
?
6.1.1.6
ATP + L-lysine + tRNALys
Borreliella burgdorferi
-
AMP + L-lysyl-tRNALys + diphosphate
-
Borreliella burgdorferi
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.1.1.6
Borreliella burgdorferi
-
purified enzyme from Bacillus subtilis strain 157.1, class I enzyme
-
6.1.1.6
Escherichia coli
-
gene lysS, class II enzyme
-
Purification (Commentary)
EC Number
Commentary
Organism
6.1.1.6
recombinant from strain BL21(DE3)
Escherichia coli
Reaction
EC Number
Reaction
Commentary
Organism
6.1.1.6
ATP + L-lysine + tRNALys = AMP + diphosphate + L-lysyl-tRNALys
active site structure
Borreliella burgdorferi
6.1.1.6
ATP + L-lysine + tRNALys = AMP + diphosphate + L-lysyl-tRNALys
active site structure
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.6
ATP + 4-aminobutanoate + tRNALys
-
652528
Escherichia coli
AMP + 4-aminobutyryl-tRNALys + diphosphate
-
652528
Escherichia coli
?
6.1.1.6
ATP + D-lysine + tRNALys
-
652528
Escherichia coli
AMP + D-lysyl-tRNALys + diphosphate
-
652528
Escherichia coli
?
6.1.1.6
ATP + L-arginine + tRNALys
low activity
652528
Escherichia coli
AMP + L-arginyl-tRNALys + diphosphate
-
652528
Escherichia coli
?
6.1.1.6
ATP + L-glutamate + tRNALys
low activity
652528
Escherichia coli
AMP + L-glutamyl-tRNALys + diphosphate
-
652528
Escherichia coli
?
6.1.1.6
ATP + L-lysinamide + tRNALys
low activity
652528
Escherichia coli
AMP + L-aminolysyl-tRNALys + diphosphate
-
652528
Escherichia coli
?
6.1.1.6
ATP + L-lysine + tRNALys
-
652528
Escherichia coli
AMP + L-lysyl-tRNALys + diphosphate
-
652528
Escherichia coli
?
6.1.1.6
ATP + L-lysine + tRNALys
-
652528
Borreliella burgdorferi
AMP + L-lysyl-tRNALys + diphosphate
-
652528
Borreliella burgdorferi
?
6.1.1.6
ATP + L-lysine + tRNALys
2-step reaction, the first step comprises the activation of the amino acid to form an enzyme-bound aminoacyl adenylate, the second step involves binding of this complex by tRNA, whose 3'-end is esterified with the aminoacyl-moiety followed by release of the resulting aminoacyl-tRNA, aminoacylation can be performed in absence of the tRNA
652528
Escherichia coli
AMP + L-lysyl-tRNALys + diphosphate
-
652528
Escherichia coli
?
6.1.1.6
ATP + L-lysine + tRNALys
2-step reaction, the first step comprises the activation of the amino acid to form an enzyme-bound aminoacyl adenylate, the second step involves binding of this complex by tRNA, whose 3'-end is esterified with the aminoacyl-moiety followed by release of the resulting aminoacyl-tRNA, aminoacylation cannot be performed in absence of the tRNA
652528
Borreliella burgdorferi
AMP + L-lysyl-tRNALys + diphosphate
-
652528
Borreliella burgdorferi
?
6.1.1.6
ATP + L-lysine ethyl ester + tRNALys
-
652528
Escherichia coli
AMP + ethyl-L-lysyl-tRNALys + diphosphate
-
652528
Escherichia coli
?
6.1.1.6
ATP + L-lysine ethyl ester + tRNALys
-
652528
Borreliella burgdorferi
AMP + ethyl-L-lysyl-tRNALys + diphosphate
-
652528
Borreliella burgdorferi
?
6.1.1.6
ATP + L-lysine hydroxamate + tRNALys
-
652528
Escherichia coli
AMP + L-lysine hydroxamoyl-tRNALys + diphosphate
-
652528
Escherichia coli
?
6.1.1.6
ATP + L-lysine methyl ester + tRNALys
-
652528
Escherichia coli
AMP + methyl-L-lysyl-tRNALys + diphosphate
-
652528
Escherichia coli
?
6.1.1.6
ATP + L-lysine methyl ester + tRNALys
-
652528
Borreliella burgdorferi
AMP + methyl-L-lysyl-tRNALys + diphosphate
-
652528
Borreliella burgdorferi
?
6.1.1.6
ATP + ornithine + tRNALys
-
652528
Escherichia coli
AMP + ornithyl-tRNALys + diphosphate
-
652528
Escherichia coli
?
6.1.1.6
ATP + ornithine + tRNALys
low activity
652528
Borreliella burgdorferi
AMP + ornithyl-tRNALys + diphosphate
-
652528
Borreliella burgdorferi
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
6.1.1.6
37
-
assay at
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.1.1.6
7.2
-
assay at
Escherichia coli
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
6.1.1.6
ATP
-
Borreliella burgdorferi
6.1.1.6
ATP
-
Escherichia coli
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
6.1.1.6
5
-
L-arginine
pH 7.2, 37°C
Borreliella burgdorferi
6.1.1.6
12
-
D-Lysine
pH 7.2, 37°C
Escherichia coli
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
6.1.1.6
expression in strain BL21(DE3)
Escherichia coli
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
6.1.1.6
ATP
-
Borreliella burgdorferi
6.1.1.6
ATP
-
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
6.1.1.6
5'-O-[N-(L-lysyl)sulfamoyl]adenosine
-
Borreliella burgdorferi
6.1.1.6
5'-O-[N-(L-lysyl)sulfamoyl]adenosine
-
Escherichia coli
6.1.1.6
AEC
competitive versus L-lysine
Escherichia coli
6.1.1.6
cadaverine
-
Escherichia coli
6.1.1.6
D-Lysine
competitive versus L-lysine
Escherichia coli
6.1.1.6
gamma-aminobutyric acid
-
Borreliella burgdorferi
6.1.1.6
L-arginine
-
Borreliella burgdorferi
6.1.1.6
lysinamide
competitive versus L-lysine
Escherichia coli
6.1.1.6
lysine ethyl ester
competitive versus L-lysine
Escherichia coli
6.1.1.6
lysine methyl ester
competitive versus L-lysine
Escherichia coli
6.1.1.6
additional information
no inhibition by lysinamide in vivo
Borreliella burgdorferi
6.1.1.6
ornithine
competitive versus L-lysine
Escherichia coli
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
6.1.1.6
5
-
L-arginine
pH 7.2, 37°C
Borreliella burgdorferi
6.1.1.6
12
-
D-Lysine
pH 7.2, 37°C
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.1.1.6
ATP + L-lysine + tRNALys
Escherichia coli
-
AMP + L-lysyl-tRNALys + diphosphate
-
Escherichia coli
?
6.1.1.6
ATP + L-lysine + tRNALys
Borreliella burgdorferi
-
AMP + L-lysyl-tRNALys + diphosphate
-
Borreliella burgdorferi
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.1.1.6
recombinant from strain BL21(DE3)
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.6
ATP + 4-aminobutanoate + tRNALys
-
652528
Escherichia coli
AMP + 4-aminobutyryl-tRNALys + diphosphate
-
652528
Escherichia coli
?
6.1.1.6
ATP + D-lysine + tRNALys
-
652528
Escherichia coli
AMP + D-lysyl-tRNALys + diphosphate
-
652528
Escherichia coli
?
6.1.1.6
ATP + L-arginine + tRNALys
low activity
652528
Escherichia coli
AMP + L-arginyl-tRNALys + diphosphate
-
652528
Escherichia coli
?
6.1.1.6
ATP + L-glutamate + tRNALys
low activity
652528
Escherichia coli
AMP + L-glutamyl-tRNALys + diphosphate
-
652528
Escherichia coli
?
6.1.1.6
ATP + L-lysinamide + tRNALys
low activity
652528
Escherichia coli
AMP + L-aminolysyl-tRNALys + diphosphate
-
652528
Escherichia coli
?
6.1.1.6
ATP + L-lysine + tRNALys
-
652528
Escherichia coli
AMP + L-lysyl-tRNALys + diphosphate
-
652528
Escherichia coli
?
6.1.1.6
ATP + L-lysine + tRNALys
-
652528
Borreliella burgdorferi
AMP + L-lysyl-tRNALys + diphosphate
-
652528
Borreliella burgdorferi
?
6.1.1.6
ATP + L-lysine + tRNALys
2-step reaction, the first step comprises the activation of the amino acid to form an enzyme-bound aminoacyl adenylate, the second step involves binding of this complex by tRNA, whose 3'-end is esterified with the aminoacyl-moiety followed by release of the resulting aminoacyl-tRNA, aminoacylation can be performed in absence of the tRNA
652528
Escherichia coli
AMP + L-lysyl-tRNALys + diphosphate
-
652528
Escherichia coli
?
6.1.1.6
ATP + L-lysine + tRNALys
2-step reaction, the first step comprises the activation of the amino acid to form an enzyme-bound aminoacyl adenylate, the second step involves binding of this complex by tRNA, whose 3'-end is esterified with the aminoacyl-moiety followed by release of the resulting aminoacyl-tRNA, aminoacylation cannot be performed in absence of the tRNA
652528
Borreliella burgdorferi
AMP + L-lysyl-tRNALys + diphosphate
-
652528
Borreliella burgdorferi
?
6.1.1.6
ATP + L-lysine ethyl ester + tRNALys
-
652528
Escherichia coli
AMP + ethyl-L-lysyl-tRNALys + diphosphate
-
652528
Escherichia coli
?
6.1.1.6
ATP + L-lysine ethyl ester + tRNALys
-
652528
Borreliella burgdorferi
AMP + ethyl-L-lysyl-tRNALys + diphosphate
-
652528
Borreliella burgdorferi
?
6.1.1.6
ATP + L-lysine hydroxamate + tRNALys
-
652528
Escherichia coli
AMP + L-lysine hydroxamoyl-tRNALys + diphosphate
-
652528
Escherichia coli
?
6.1.1.6
ATP + L-lysine methyl ester + tRNALys
-
652528
Escherichia coli
AMP + methyl-L-lysyl-tRNALys + diphosphate
-
652528
Escherichia coli
?
6.1.1.6
ATP + L-lysine methyl ester + tRNALys
-
652528
Borreliella burgdorferi
AMP + methyl-L-lysyl-tRNALys + diphosphate
-
652528
Borreliella burgdorferi
?
6.1.1.6
ATP + ornithine + tRNALys
-
652528
Escherichia coli
AMP + ornithyl-tRNALys + diphosphate
-
652528
Escherichia coli
?
6.1.1.6
ATP + ornithine + tRNALys
low activity
652528
Borreliella burgdorferi
AMP + ornithyl-tRNALys + diphosphate
-
652528
Borreliella burgdorferi
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
6.1.1.6
37
-
assay at
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.1.1.6
7.2
-
assay at
Escherichia coli