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Literature summary extracted from

  • Kouchi, Z.; Fukami, K.; Shikano, T.; Oda, S.; Nakamura, Y.; Takenawa, T.; Miyazaki, S.
    Recombinant phospholipase Czeta has high Ca2+ sensitivity and induces Ca2+ oscillations in mouse eggs (2004), J. Biol. Chem., 279, 10408-10412.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.11 microinjection of recombinant PLCzeta protein into mouse eggs induces serial Ca2+ spikes quite similar to that produced by the injection of sperm extract, probably because of repetitive Ca2+ release from the endoplasmic reticulum caused by continuously produced inositol 1,4,5-trisphosphate Mus musculus
3.1.4.11 PLC-delta1 Mus musculus
3.1.4.11 PLCdelta1, expression in Sf9 cells Mus musculus
3.1.4.11 PLCzeta, expression in Sf9 cells Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.11 additional information PLCzeta mutant lacking three EF-hand domains Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.11 Ca2+ activates PLCdelta1, EC50: 0.0057 mM, PLCdelta1 has a 100fold lower Ca2+ sensitivity than PLCzeta Mus musculus
3.1.4.11 Ca2+ PLCzeta, 70% of maximal activity at 100 nM Ca2+, EC50: 52 nM, PLCzeta has a 100fold higher Ca2+ sensitivity than PLCdelta1 Mus musculus
3.1.4.11 Ca2+ activity of PLC-delta1 increases at a Ca2+ concentration between 0.001 and 0.03 mM Mus musculus
3.1.4.11 Ca2+ PLCzeta exhibites a significant activity at Ca2+ concentration of 10 nM and has 70% maximal activity at 100 nM Ca2+ that is the basal intracellular calcium ion concentration level of cells Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.11 78000
-
x * 78000, recombinant His-tagged PLCzeta, SDS-PAGE Mus musculus
3.1.4.11 90000
-
x * 90000, recombinant His-tagged PLCdelta1, SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.11 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O Mus musculus sperm-specific PLCzeta induces intracellular Ca2+ oscillations and subsequent early embryonic development when expressed in mouse eggs, PLCzeta may be the physiological mammalian egg-activating sperm factor 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
-
?
3.1.4.11 additional information Mus musculus recombinant PLCzeta protein induces Ca2+ oscillations in mouse eggs ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.11 Mus musculus
-
-
-
3.1.4.11 Mus musculus Q8K4D7
-
-
3.1.4.11 Mus musculus Q8K4D7 PLCzeta
-
3.1.4.11 Mus musculus Q8R3B1 PLCdelta1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.11 enzyme PLC-delta1 prepared by a baculovirus expression system Mus musculus
3.1.4.11 enzyme prepared by a baculovirus expression system Mus musculus
3.1.4.11 recombinant PLCdelta1 Mus musculus
3.1.4.11 recombinant PLCzeta Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.11 egg
-
Mus musculus
-
3.1.4.11 sperm sperm-specific PLCzeta Mus musculus
-
3.1.4.11 testis
-
Mus musculus
-
3.1.4.11 testis PLCzeta Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.11 1.3
-
PLCzeta, at 0.001 mM Ca2+, pH 7, 37°C Mus musculus
3.1.4.11 1.3
-
at 0.001 mM Ca2+ Mus musculus
3.1.4.11 65
-
PLCdelta1, at 0.03 mM Ca2+, pH 7, 37°C Mus musculus
3.1.4.11 65
-
at 0.03 mM Ca2+, PLC-delta1 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.11 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
-
Mus musculus 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
-
?
3.1.4.11 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O domain structure of PLCdelta1 Mus musculus 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
-
?
3.1.4.11 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O domain structure of PLCzeta Mus musculus 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
-
?
3.1.4.11 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O sperm-specific PLCzeta induces intracellular Ca2+ oscillations and subsequent early embryonic development when expressed in mouse eggs, PLCzeta may be the physiological mammalian egg-activating sperm factor Mus musculus 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
-
?
3.1.4.11 additional information recombinant PLCzeta protein induces Ca2+ oscillations in mouse eggs Mus musculus ?
-
?

Subunits

EC Number Subunits Comment Organism
3.1.4.11 ? x * 78000, recombinant His-tagged PLCzeta, SDS-PAGE Mus musculus
3.1.4.11 ? x * 90000, recombinant His-tagged PLCdelta1, SDS-PAGE Mus musculus

Synonyms

EC Number Synonyms Comment Organism
3.1.4.11 PLC-delta1
-
Mus musculus
3.1.4.11 PLC-zeta
-
Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.11 37
-
assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.11 7
-
assay at Mus musculus