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Literature summary extracted from
- Novel natural peptide substrates for endopeptidase 24.15, neurolysin, and angiotensin-converting enzyme (2003), J. Biol. Chem., 278, 8547-8555.
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.4.24.15 | E502A | complete loss of enzymatic activity | Rattus norvegicus |
| 3.4.24.15 | H473A | complete loss of enzymatic activity | Rattus norvegicus |
| 3.4.24.15 | H474A | complete loss of enzymatic activity | Rattus norvegicus |
| 3.4.24.15 | H477A | complete loss of enzymatic activity | Rattus norvegicus |
| 3.4.24.16 | E502A | complete loss of enzymatic activity | Rattus norvegicus |
| 3.4.24.16 | H473A | complete loss of enzymatic activity | Rattus norvegicus |
| 3.4.24.16 | H474A | complete loss of enzymatic activity | Rattus norvegicus |
| 3.4.24.16 | H477A | complete loss of enzymatic activity | Rattus norvegicus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.15 | angiotensin I | inhibits hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl) | Rattus norvegicus |  |
| 3.4.24.15 | angiotensin II | inhibits hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl) | Rattus norvegicus | |
| 3.4.24.15 | bradykinin | inhibits hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl) | Rattus norvegicus | |
| 3.4.24.15 | dynorphin A1-13 | inhibits hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl) | Rattus norvegicus | |
| 3.4.24.15 | LVVYPWTQRY | inhibits hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl) | Rattus norvegicus | |
| 3.4.24.15 | PVNFKFLSH | inhibits hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl) | Rattus norvegicus | |
| 3.4.24.15 | VVYPWTQRY | inhibits hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl) | Rattus norvegicus | |
| 3.4.24.16 | angiotensin I | inhibits hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl) | Rattus norvegicus | |
| 3.4.24.16 | angiotensin II | inhibits hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl) | Rattus norvegicus | |
| 3.4.24.16 | bradykinin | inhibits hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl) | Rattus norvegicus | |
| 3.4.24.16 | dynorphin A1-13 | inhibits hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl) | Rattus norvegicus | |
| 3.4.24.16 | LVVYPWTQRY | inhibits hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl) | Rattus norvegicus | |
| 3.4.24.16 | PVNFKFLSH | inhibits hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl) | Rattus norvegicus | |
| 3.4.24.16 | VVYPWTQRY | inhibits hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl) | Rattus norvegicus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.24.15 | Rattus norvegicus | - |
- |
- |
| 3.4.24.16 | Rattus norvegicus | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.24.15 | testis | - |
Rattus norvegicus | - |
| 3.4.24.16 | testis | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.15 | 7-methoxycoumatin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl) + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.4.24.15 | angiotensin I + H2O | 33.5% of the activity with bradykinin | Rattus norvegicus | ? | - |
? | |
| 3.4.24.15 | angiotensin II + H2O | 7% of the activity with bradykinin | Rattus norvegicus | ? | - |
? | |
| 3.4.24.15 | bradykinin + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.4.24.15 | LVVYPWTQRY + H2O | 0.72% of the activity with bradykinin, cleavage sites: LVVYP-/-W-/-T-/-Q-/-RY | Rattus norvegicus | ? | - |
? | |
| 3.4.24.15 | PVNFKFLSH + H2O | 140% of the activity with bradykinin, cleavage sites: PVNF-/-K-/-F-/-LSH | Rattus norvegicus | ? | - |
? | |
| 3.4.24.15 | VVYPWTQRY + H2O | 10.6% of the activity with bradykinin, cleavage sites: VVYPW-/-T-/-Q-/-RY | Rattus norvegicus | ? | - |
? | |
| 3.4.24.16 | 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl) + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.4.24.16 | angiotensin I + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.4.24.16 | angiotensin II + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.4.24.16 | bradykinin + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.4.24.16 | dynorphin A1-13 + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.4.24.16 | LVVYPWTQRY + H2O | cleavage sites: LVVYP-/-W-/-T-/-Q-/-RY | Rattus norvegicus | ? | - |
? | |
| 3.4.24.16 | PVNFKFLSH + H2O | cleavage sites: PVNF-/-K-/-F-/-LSH | Rattus norvegicus | ? | - |
? | |
| 3.4.24.16 | VVYPWTQRY + H2O | cleavage sites: VVYPW-/-T-/-Q-/-RY | Rattus norvegicus | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.24.15 | endopeptidase 24.15 | - |
Rattus norvegicus |
| 3.4.24.15 | EP24.15 | - |
Rattus norvegicus |
| 3.4.24.16 | endopeptidase 24.16 | - |
Rattus norvegicus |
| 3.4.24.16 | ep24.16 | - |
Rattus norvegicus |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.24.15 | 0.00004 | - |
dynorphin A1-13 | inhibition of hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl) | Rattus norvegicus | |
| 3.4.24.15 | 0.00256 | - |
LVVYPWTQRY | inhibition of hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl) | Rattus norvegicus | |
| 3.4.24.15 | 0.00429 | - |
angiotensin I | inhibition of hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl) | Rattus norvegicus | |
| 3.4.24.15 | 0.00536 | - |
bradykinin | inhibition of hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl) | Rattus norvegicus | |
| 3.4.24.15 | 0.00812 | - |
angiotensin II | inhibition of hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl) | Rattus norvegicus | |
| 3.4.24.15 | 0.01002 | - |
VVYPWTQRY | inhibition of hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl) | Rattus norvegicus | |
| 3.4.24.15 | 0.02776 | - |
PVNFKFLSH | inhibition of hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl) | Rattus norvegicus | |
| 3.4.24.16 | 0.000022 | - |
dynorphin A1-13 | inhibition of hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl) | Rattus norvegicus | |
| 3.4.24.16 | 0.00201 | - |
LVVYPWTQRY | inhibition of hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl) | Rattus norvegicus | |
| 3.4.24.16 | 0.00343 | - |
PVNFKFLSH | inhibition of hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl) | Rattus norvegicus | |
| 3.4.24.16 | 0.00535 | - |
angiotensin I | inhibition of hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl) | Rattus norvegicus | |
| 3.4.24.16 | 0.00704 | - |
VVYPWTQRY | inhibition of hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl) | Rattus norvegicus | |
| 3.4.24.16 | 0.00795 | - |
angiotensin II | inhibition of hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl) | Rattus norvegicus | |
| 3.4.24.16 | 0.00811 | - |
bradykinin | inhibition of hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl) | Rattus norvegicus | |
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