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Literature summary extracted from

  • Wolucka, B.A.; Van Montagu, M.
    GDP-mannose 3',5'-epimerase forms GDP-L-gulose, a putative intermediate for the de novo biosynthesis of vitamin C in plants (2003), J. Biol. Chem., 278, 47483-47490.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.3.18 expression in Escherichia coli Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.3.18 GDP potent competitive inhibitor Arabidopsis thaliana
5.1.3.18 GDP-D-glucose potent competitive inhibitor Arabidopsis thaliana
5.1.3.18 GDP-L-fucose complex type of inhibition, IC50: 0.07 mM Arabidopsis thaliana
5.1.3.18 L-ascorbic acid 1 mM, 15% inhibition Arabidopsis thaliana
5.1.3.18 L-galactono-1,4-lactone 1 mM, 14% inhibition Arabidopsis thaliana
5.1.3.18 NADH
-
 Arabidopsis thaliana
5.1.3.18 NADPH
-
 Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.3.18 0.0045
-
 GDP-D-mannose native enzyme Arabidopsis thaliana
5.1.3.18 0.018
-
 GDP-D-mannose recombinant enzyme with N-terminal His-tag Arabidopsis thaliana
5.1.3.18 0.031
-
 GDP-D-mannose recombinant enzyme with N-terminal glutathione-S-transferase tag Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.3.18 GDP-D-mannose Arabidopsis thaliana the enzyme catalyzes a reversible epimerization that precedes the committed step in the biosynthesis of vitamin C, resulting in the hydrolysis of the highly energetic glycosyl-diphosphoryl linkage. The plant enzyme undergoes a complex regulation and could control the carbonflux into the vitamin C pathway in response to the redox state of thecell, stress conditions, and GDP-sugar demand for the cell wall glycoprotein biosynthesis GDP-L-galactose
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.18 Arabidopsis thaliana
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.18 GDP-D-mannose the reaction mixture contains at equilibrium GDP-D-mannose, GDP-L-galactose and GDP-L-gulose in a ratio of 1:0.4:0.2 Arabidopsis thaliana GDP-L-galactose
-
 r
5.1.3.18 GDP-D-mannose the enzyme catalyzes a reversible epimerization that precedes the committed step in the biosynthesis of vitamin C, resulting in the hydrolysis of the highly energetic glycosyl-diphosphoryl linkage. The plant enzyme undergoes a complex regulation and could control the carbonflux into the vitamin C pathway in response to the redox state of thecell, stress conditions, and GDP-sugar demand for the cell wall glycoprotein biosynthesis Arabidopsis thaliana GDP-L-galactose
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.1.3.18 0.007
-
 GDP-D-mannose recombinant enzyme with N-terminal His-tag Arabidopsis thaliana
5.1.3.18 0.01
-
 GDP-D-mannose recombinant enzyme with N-terminal glutathione-S-transferase tag Arabidopsis thaliana
5.1.3.18 0.041
-
 GDP-D-mannose native enzyme Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
5.1.3.18 NAD+ stimulates Arabidopsis thaliana
5.1.3.18 NADP+ stimulates Arabidopsis thaliana

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
5.1.3.18 0.07
-
 complex type of inhibition, IC50: 0.07 mM Arabidopsis thaliana GDP-L-fucose