EC Number | Cloned (Comment) | Organism |
---|---|---|
4.4.1.11 | expression of EhMGL1 in Escherichia coli | Entamoeba histolytica |
4.4.1.11 | expression of EhMGL2 in Escherichia coli | Entamoeba histolytica |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.4.1.11 | DL-propargylglycine | irreversible slow-binding inhibition | Entamoeba histolytica |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.4.1.11 | 0.94 | - |
L-methionine | 36°C, pH 6.8, recombinant EhMGL1 | Entamoeba histolytica | |
4.4.1.11 | 1.87 | - |
DL-homocysteine | 36°C, pH 6.8, recombinant EhMGL2 | Entamoeba histolytica | |
4.4.1.11 | 1.9 | - |
L-methionine | 36°C, pH 6.8, recombinant EhMGL2 | Entamoeba histolytica | |
4.4.1.11 | 3.4 | - |
DL-homocysteine | 36°C, pH 6.8, recombinant EhMGL1 | Entamoeba histolytica |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
4.4.1.11 | cytosol | - |
Entamoeba histolytica | 5829 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.4.1.11 | 43300 | - |
4 * 43300, SDS-PAGE, immunoblot, native and recombinant EhMGL1 form homotetramers | Entamoeba histolytica |
4.4.1.11 | 43700 | - |
4 * 43700, SDS-PAGE, immunoblot, native and recombinant EhMGL2 form homotetramers | Entamoeba histolytica |
4.4.1.11 | 171000 | 177000 | gel filtration, native and recombinant EhMGL1 | Entamoeba histolytica |
4.4.1.11 | 171000 | 177000 | gel filtration, native and recombinant EhMGL2 | Entamoeba histolytica |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.4.1.11 | L-methionine + H2O | Entamoeba histolytica | - |
methanethiol + NH3 + 2-oxobutanoate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.4.1.11 | Entamoeba histolytica | Q86D27 | isoenzyme 2 | - |
4.4.1.11 | Entamoeba histolytica | Q86D28 | isoenzyme 1 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.4.1.11 | recombinant EhMGL1 | Entamoeba histolytica |
4.4.1.11 | recombinant EhMGL2 | Entamoeba histolytica |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.4.1.11 | 0.36 | - |
recombinant EhMGL1 | Entamoeba histolytica |
4.4.1.11 | 0.44 | - |
recombinant EhMGL2 | Entamoeba histolytica |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.4.1.11 | DL-homocysteine + H2O | 112% of activity with L-methionine | Entamoeba histolytica | 2-oxobutanoate + NH3 + H2S | - |
? | |
4.4.1.11 | DL-homocysteine + H2O | 294% of EhMGL1 activity with L-methionine | Entamoeba histolytica | 2-oxobutanoate + NH3 + H2S | - |
? | |
4.4.1.11 | DL-homocysteine + H2O | - |
Entamoeba histolytica | hydrogen sulfide + NH3 + 2-oxobutanoate | - |
? | |
4.4.1.11 | L-cysteine + H2O | 160% of EhMGL1 activity with L-methionine | Entamoeba histolytica | sulfide + NH3 + pyruvate | - |
? | |
4.4.1.11 | L-cysteine + H2O | 20% of activity with L-methionine | Entamoeba histolytica | sulfide + NH3 + pyruvate | - |
? | |
4.4.1.11 | L-methionine + H2O | - |
Entamoeba histolytica | methanethiol + NH3 + 2-oxobutanoate | - |
? | |
4.4.1.11 | L-methionine + H2O | 181% of EhMGL1 activity with L-methionine | Entamoeba histolytica | methanethiol + NH3 + 2-oxobutanoate | - |
? | |
4.4.1.11 | O-acetyl-L-serine + H2O | 11% of activity with L-methionine | Entamoeba histolytica | 2-oxopropanoate + NH3 + acetate | - |
? | |
4.4.1.11 | O-acetyl-L-serine + H2O | 34% of EhMGL1 activity with L-methionine | Entamoeba histolytica | 2-oxopropanoate + NH3 + acetate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.4.1.11 | tetramer | 4 * 43300, SDS-PAGE, immunoblot, native and recombinant EhMGL1 form homotetramers | Entamoeba histolytica |
4.4.1.11 | tetramer | 4 * 43700, SDS-PAGE, immunoblot, native and recombinant EhMGL2 form homotetramers | Entamoeba histolytica |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.4.1.11 | EhMGL1 | methionine gamma-lyase isoenzyme 1 | Entamoeba histolytica |
4.4.1.11 | EhMGL2 | methionine gamma-lyase isoenzyme 2 | Entamoeba histolytica |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.4.1.11 | 7 | - |
- |
Entamoeba histolytica |
4.4.1.11 | 7.7 | - |
- |
Entamoeba histolytica |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
4.4.1.11 | 5.6 | 8.2 | approx. 14% of maximal activity at pH 5.6, approx. 10% of maximal activity at pH 8.2 | Entamoeba histolytica |
4.4.1.11 | 5.6 | 8.2 | approx. 55% of maximal activity at pH 5.6, approx. 95% of maximal activity at pH 8.2 | Entamoeba histolytica |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.4.1.11 | 0.035 | - |
DL-propargylglycine | - |
Entamoeba histolytica |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
4.4.1.11 | Entamoeba histolytica | two-dimensional PAGE, recombinant EhMGL1 | - |
5.9 |
4.4.1.11 | Entamoeba histolytica | two-dimensional PAGE, native EhMGL1 | - |
6 |
4.4.1.11 | Entamoeba histolytica | two-dimensional PAGE, recombinant EhMGL2 | - |
6.5 |
4.4.1.11 | Entamoeba histolytica | two-dimensional PAGE, native EhMGL2 | - |
6.7 |