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Literature summary extracted from
- Extended substrate specificity of rat mast cell protease 5, a rodent alpha-chymase with elastase-like primary specificity (2003), J. Biol. Chem., 278, 39625-39631.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.21.B5 | - |
Rattus norvegicus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.B5 | alpha-1-antitrypsin | forms a covalent complex with the enzyme, heparin protects complex formation | Rattus norvegicus | |
| 3.4.21.B5 | diisopropyl fluorophosphates | binds covalently to the enzyme | Rattus norvegicus |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.B5 | 3.1 | - |
succinyl-Ala-Ala-Pro-Ile-4-nitroanilide | pH 8.5 | Rattus norvegicus | |
| 3.4.21.B5 | 4 | - |
succinyl-Ala-Ala-Pro-Val-4-nitroanilide | pH 8.5 | Rattus norvegicus | |
| 3.4.21.B5 | 7.6 | - |
succinyl-Ala-Ala-Pro-Ala-4-nitroanilide | pH 8.5 | Rattus norvegicus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.B5 | 25390 | - |
calculated from amino acid sequence | Rattus norvegicus |
| 3.4.21.B5 | 32000 | - |
SDS-PAGE | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.B5 | Protein + H2O | Rattus norvegicus | - |
? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.B5 | Rattus norvegicus | P50339 | - |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.21.B5 | glycoprotein | - |
Rattus norvegicus |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.21.B5 | - |
Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.B5 | Protein + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.4.21.B5 | succinyl-Ala-Ala-Pro-Ala-4-nitroanilide + H2O | highest activity | Rattus norvegicus | ? | - |
? | |
| 3.4.21.B5 | succinyl-Ala-Ala-Pro-Ile-4-nitroanilide + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.4.21.B5 | succinyl-Ala-Ala-Pro-Leu-4-nitroanilide + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.4.21.B5 | succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O | low activity | Rattus norvegicus | ? | - |
? | |
| 3.4.21.B5 | succinyl-Ala-Ala-Pro-Val-4-nitroanilide + H2O | highest activity | Rattus norvegicus | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.21.B5 | alpha-chymase | - |
Rattus norvegicus |
| 3.4.21.B5 | rat mast cell protease 5 | - |
Rattus norvegicus |
| 3.4.21.B5 | rMCP-5 | - |
Rattus norvegicus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.B5 | 0.0072 | - |
succinyl-Ala-Ala-Pro-Ile-4-nitroanilide | pH 8.5 | Rattus norvegicus | |
| 3.4.21.B5 | 0.017 | - |
succinyl-Ala-Ala-Pro-Val-4-nitroanilide | pH 8.5 | Rattus norvegicus | |
| 3.4.21.B5 | 0.029 | - |
succinyl-Ala-Ala-Pro-Ala-4-nitroanilide | pH 8.5 | Rattus norvegicus | |
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