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Literature summary extracted from

  • Johanson, K.; Hoang, T.; Sheth, M.; Hyman, L.E.
    GRS1, a yeast tRNA synthetase with a role in mRNA 3' end formation (2003), J. Biol. Chem., 278, 35923-35930.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.14 expression of His-tagged wild-type and p552F mutant enzyme in Escherichia coli Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.14 P552F temperature-sensitive mutant grs1-1 shows altered substrate specificities, the mutant strain can be complemented by expression of the wild-type enzyme Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.14 additional information
-
 additional information dissociation constants for complexes of wild-type or mutant enzyme with tRNA and mRNA Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.14 72000
-
 x * 72000, wild-type enzyme, SDS-PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.14 ATP + glycine + tRNAGly Saccharomyces cerevisiae
-
 AMP + diphosphate + glycyl-tRNAGly
-
 ?
6.1.1.14 additional information Saccharomyces cerevisiae enzyme plays an important role in transcription termination by affecting 3'-end formation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.14 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.14 recombinant His-atgged wild-type enzyme and P552F mutant from Escherichia coli Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.14 additional information
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.14 ATP + glycine + tRNAGly
-
 Saccharomyces cerevisiae AMP + diphosphate + glycyl-tRNAGly
-
 ?
6.1.1.14 ATP + glycine + tRNAGly wild-type and mutant tRNAGly substrates Saccharomyces cerevisiae AMP + diphosphate + glycyl-tRNAGly
-
 ?
6.1.1.14 additional information enzyme binds the 3'-ends of mRNA from yeast competing with the cognate tRNAGly substrate Saccharomyces cerevisiae ?
-
 ?
6.1.1.14 additional information enzyme plays an important role in transcription termination by affecting 3'-end formation Saccharomyces cerevisiae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.14 ? x * 72000, wild-type enzyme, SDS-PAGE Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
6.1.1.14 Glycine--tRNA ligase
-
 Saccharomyces cerevisiae
6.1.1.14 Glycyl translase
-
 Saccharomyces cerevisiae
6.1.1.14 Glycyl-transfer ribonucleate synthetase
-
 Saccharomyces cerevisiae
6.1.1.14 Glycyl-transfer ribonucleic acid synthetase
-
 Saccharomyces cerevisiae
6.1.1.14 Glycyl-transfer RNA synthetase
-
 Saccharomyces cerevisiae
6.1.1.14 Glycyl-tRNA synthetase
-
 Saccharomyces cerevisiae
6.1.1.14 GlyRS
-
 Saccharomyces cerevisiae
6.1.1.14 Synthetase, glycyl-transfer ribonucleate
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.14 30
-
 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.14 7.2
-
 assay at Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.14 ATP
-
 Saccharomyces cerevisiae