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Literature summary extracted from

  • Thomas, J.L.; Duax, W.L.; Addlagatta, A.; Brandt, S.; Fuller, R.R.; Norris, W.
    Structure/function relationships responsible for coenzyme specificity and the isomerase activity of human type 1 3 beta-hydroxysteroid dehydrogenase/isomerase (2003), J. Biol. Chem., 278, 35483-35490.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.3.1 mutant enzymes D36A/K37R, D241N, D257L, D258L and D265N are expressed in a baculovirus/Sf9 cell system Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
5.3.3.1 D241N mutant enzyme nearly has full isomerase activity, substantial decrease in 3beta-hydroxysteroid dehydrogenase activity. Mutant enzyme has a basal isomerase activity in the absence of coenzyme that is 10% of the NADH-stimulated turnover number Homo sapiens
5.3.3.1 D257L complete lack of isomerase activity in absence of coenzyme, substantial decrease in 3beta-hydroxysteroid dehydrogenase activity Homo sapiens
5.3.3.1 D258L complete lack of isomerase activity in absence of coenzyme, substantial decrease in 3beta-hydroxysteroid dehydrogenase activity Homo sapiens
5.3.3.1 D265N complete lack of isomerase activity in absence of coenzyme, substantial decrease in 3beta-hydroxysteroid dehydrogenase activity Homo sapiens
5.3.3.1 D36A/K37R mutation shifts the cofactor preference of both 3-beta-hydroxysteroid dehydrogenase and isomerase from NAD(H) to NADP(H) Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.3.1 0.0024
-
 5-androstene-3,17-dione 27°C, pH 7.4, wild-type enzyme Homo sapiens
5.3.3.1 0.0033
-
 5-androstene-3,17-dione 27°C, pH 7.4, mutant enzyme D241N Homo sapiens
5.3.3.1 0.0044
-
 5-androstene-3,17-dione 27°C, pH 7.4, mutant enzyme D265N Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
5.3.3.1 Homo sapiens
-
 type I 3beta-hydroxysteroid dehydrogenase/isomerase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.3.1 mutant enzymes D36A/K37R, D241N, D257L, D258L and D265N are expressed in a baculovirus/Sf9 cell system Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.3.1 5-Androstene-3,17-dione
-
 Homo sapiens 4-Androstene-3,17-dione
-
 ?
5.3.3.1 dehydroepiandrosterone
-
 Homo sapiens 3beta-hydroxy-4-androsten-17-one
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.3.3.1 3beta-HSD/isomerase
-
 Homo sapiens
5.3.3.1 type I 3beta-hydroxysteroid dehydrogenase/isomerase
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.3.1 0.295
-
 5-androstene-3,17-dione 27°C, pH 7.4, mutant enzyme D265N Homo sapiens
5.3.3.1 0.48
-
 5-androstene-3,17-dione 27°C, pH 7.4, mutant enzyme D241N Homo sapiens
5.3.3.1 0.557
-
 5-androstene-3,17-dione 27°C, pH 7.4, wild-type enzyme Homo sapiens
5.3.3.1 6.08
-
 5-androstene-3,17-dione 27°C, pH 7.4, wild-type enzyme Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.3.1 7.5
-
 wild-type 1 isomerase and mutant enzymes D241N and D265N Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.3.3.1 6.5 8 pH 6.5: about 55% of maximal activity, wild-type enzyme and mutant enzyme D265N, about 65% of maximal activity, mutant enzyme D241N, about 85% of maximal activity mutant enzyme D257L and D258L. pH 8.0: about 50% of maximal activity, wild-type enzyme and mutant enzymes D241N, 95% of maximal activity, mutant enzyme D258L Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
5.3.3.1 NADH wild-type 1 isomerase is activated only by NADH with NADPH producing no isomerase activity. D36A/K37R mutation shifts the cofactor preference of both 3-beta-hydroxysteroid dehydrogenase and isomerase from NAD(H) to NADP(H) Homo sapiens
5.3.3.1 NADPH D36A/K37R mutation shifts the cofactor preference of both 3-beta-hydroxysteroid dehydrogenase and isomerase from NAD(H) to NADP(H) Homo sapiens