EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.6.8 | plasmids containing the wild-type and mutant cDNAs are transiently transfected into BHK cells | Homo sapiens |
3.1.6.8 | wild-type and mutant enzymes transiently transfected into BHK cells | Homo sapiens |
3.1.6.12 | wild-type and mutant enzymes transiently transfected into BHK cells | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.6.8 | K302A | 34% enzyme activity in comparison to wild-type enzyme | Homo sapiens |
3.1.6.8 | K302A | 34% of the activity of wild-type enzyme when expressed in BHK cells. Mutation has no effect on distribution of the enzyme activity between cell and medium | Homo sapiens |
3.1.6.8 | K367A | 60% enzyme activity in comparison to wild-type enzyme | Homo sapiens |
3.1.6.8 | K367A | 60% of the activity of wild-type enzyme when expressed in BHK cells. Mutation has no effect on distribution of the enzyme activity between cell and medium. 106% of the mannose-phosphorylation of the wild-type enzyme | Homo sapiens |
3.1.6.8 | K393A | 39% enzyme activity in comparison to wild-type enzyme | Homo sapiens |
3.1.6.8 | K393A | 39% of the activity of wild-type enzyme when expressed in BHK cells. Mutation has no effect on distribution of the enzyme activity between cell and medium. 123% of the mannose-phosphorylation of the wild-type enzyme | Homo sapiens |
3.1.6.8 | K393A/K395G | 35% enzyme activity in comparison to wild-type enzyme | Homo sapiens |
3.1.6.8 | K393A/K395G | 35% of the activity of wild-type enzyme when expressed in BHK cells. Mutation has no effect on distribution of the enzyme activity between cell and medium | Homo sapiens |
3.1.6.8 | K393A/K395H | 30% enzyme activity in comparison to wild-type enzyme | Homo sapiens |
3.1.6.8 | K393A/K395H | 30% of the activity of wild-type enzyme when expressed in BHK cells. Mutation has no effect on distribution of the enzyme activity between cell and medium | Homo sapiens |
3.1.6.8 | K433A | 102% enzyme activity in comparison to wild-type enzyme | Homo sapiens |
3.1.6.8 | K433A | 102% of the activity of wild-type enzyme when expressed in BHK cells. Mutation has no effect on distribution of the enzyme activity between cell and medium. 95% of the mannose-phosphorylation of the wild-type enzyme | Homo sapiens |
3.1.6.8 | K457G | 7.5% enzyme activity in comparison to wild-type enzyme | Homo sapiens |
3.1.6.8 | K457G | 7.5% of the activity of wild-type enzyme when expressed in BHK cells. Mutation leads to an increase of enzyme activity in the medium to about 60% of total activity compared to 35% of the wild-type enzyme. 31% of the mannose-phosphorylation of the wild-type enzyme. Mutation affects phosphorylation of oligosaccharide Asn350 to a greater extent than that of oligosaccharide Asn158 | Homo sapiens |
3.1.6.8 | K457R | 45% enzyme activity in comparison to wild-type enzyme | Homo sapiens |
3.1.6.8 | K457R | 45% of the activity of wild-type enzyme when expressed in BHK cells. Mutation leads to an increase of enzyme activity in the medium to about 60% of total activity compared to 35% of the wild-type enzyme. 33% of the mannose-phosphorylation of the wild-type enzyme. Mutation affects phosphorylation of oligosaccharide Asn350 to a greater extent than that of oligosaccharide Asn158 | Homo sapiens |
3.1.6.8 | K457S | 25% enzyme activity in comparison to wild-type enzyme | Homo sapiens |
3.1.6.8 | K457S | 25% of the activity of wild-type enzyme when expressed in BHK cells. 50% of the mannose-phosphorylation of the wild-type enzyme. Mutation leads to an increase of enzyme activity in the medium to about 65% of total activity compared to 35% of the wild-type enzyme. 50% of the mannose-phosphorylation of the wild-type enzyme. Mutation affects phosphorylation of oligosaccharide Asn350 to a greater extent than that of oligosaccharide Asn158 | Homo sapiens |
3.1.6.8 | K463Q | 14% enzyme activity in comparison to wild-type enzyme | Homo sapiens |
3.1.6.8 | K463Q | 14% of the activity of wild-type enzyme when expressed in BHK cells. Mutation leads to an decrease of enzyme activity in the medium to about 25% of total activity compared to 35% of the wild-type enzyme | Homo sapiens |
3.1.6.8 | K463R | 39% enzyme activity in comparison to wild-type enzyme | Homo sapiens |
3.1.6.8 | K463R | 39% of the activity of wild-type enzyme when expressed in BHK cells. 31% of the mannose-phosphorylation of the wild-type enzyme. Mutation leads to an decrease of enzyme activity in the medium to about 25% of total activity compared to 35% of the wild-type enzyme | Homo sapiens |
3.1.6.8 | additional information | only substitution of Lys457 causes a reduction of phosphorylation to 33% and increases secretion of the mutant enzyme | Homo sapiens |
3.1.6.8 | N158Q/N350Q | 43% enzyme activity in comparison to wild-type enzyme | Homo sapiens |
3.1.6.8 | N158Q/N350Q | 43% of the activity of wild-type enzyme when expressed in BHK cells. Mutation leads to an increase of enzyme activity in the medium to about 25% of total activity compared to 75% of the wild-type enzyme | Homo sapiens |
3.1.6.12 | K470A | mutation leads to an decrease of enzyme activity in the medium to 13% of total activity compared to 25% of the wild-type enzyme, no effect on phosphorylation | Homo sapiens |
3.1.6.12 | K470A/K497A/K507A | mutation leads to an decrease of enzyme activity in the medium to 17% of total activity compared to 25% of the wild-type enzyme, no effect on phosphorylation | Homo sapiens |
3.1.6.12 | K470A/K507A | mutation leads to an decrease of enzyme activity in the medium to 16% of total activity compared to 25% of the wild-type enzyme, no effect on phosphorylation | Homo sapiens |
3.1.6.12 | K497A | mutation leads to an decrease of enzyme activity in the medium to 19% of total activity compared to 25% of the wild-type enzyme, no effect on phosphorylation | Homo sapiens |
3.1.6.12 | K497A/K507A | mutation leads to an decrease of enzyme activity in the medium to 18% of total activity compared to 25% of the wild-type enzyme, no effect on phosphorylation | Homo sapiens |
3.1.6.12 | K507A | mutation leads to an decrease of enzyme activity in the medium to 23% of total activity compared to 25% of the wild-type enzyme, no effect on phosphorylation | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.6.8 | lysosome | - |
Homo sapiens | 5764 | - |
3.1.6.12 | lysosome | - |
Homo sapiens | 5764 | - |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.6.8 | Homo sapiens | - |
- |
- |
3.1.6.12 | Homo sapiens | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.1.6.8 | glycoprotein | the wild-type enzyme has three N-glycosylation sites. Only oligosaccharides at the first, Asn158, and the third, Asn350, glycosylation site are phosphorylated, whereas the second, Asn184 is not | Homo sapiens |
3.1.6.8 | phosphoprotein | compared to the degree of mannose-phosphorylation of the wild-type enzyme, the mutant enzyme K457R has 33%, mutant enzyme K457S has 50%, mutant enzyme K457G has 31%, mutant enzyme K433A has 95%, mutant enzyme K367A has 106% and mutant enzyme K393A has 123% of mannose-phosphorylation respectively | Homo sapiens |
3.1.6.12 | glycoprotein | the wild-type enzyme has three N-glycosylation sites. Only oligosaccharides at the first, Asn158, and the third, Asn350, glycosylation site are phosphorylated, whereas the second, Asn184 is not | Homo sapiens |
3.1.6.12 | phosphoprotein | compared to degree of mannose-phosphorylation of the wild-type enzyme the mutant enzyme K457R has 33%, mutant enzyme K457S has 50%, mutant enzyme K457G has 31% mutant enzyme K433A has 95%, mutant enzyme K367A has 106% and mutant enzyme K393A has 123% of mannose-phosphorylation | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.6.8 | cerebroside 3-sulfate + H2O | 3-O-sulfogalactosyl ceramide | Homo sapiens | cerebroside + sulfate | galactosyl ceramide | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.6.8 | ASA | - |
Homo sapiens |
3.1.6.12 | ASB | - |
Homo sapiens |