BRENDA - Enzyme Database show

A structural basis for half-of-the-sites metal binding revealed in Drosophila melanogaster porphobilinogen synthase

Kundrat, L.; Martins, J.; Stith, L.; Dunbrack, R.L., Jr.; Jaffe, E.K.; J. Biol. Chem. 278, 31325-31330 (2003)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.24
-
Drosophila melanogaster
Engineering
EC Number
Amino acid exchange
Commentary
Organism
4.2.1.24
H10F
mutant enzyme is active but is not inhibited by zinc. H10F binds a catalytic zinc at 0.5/subunit and binds a second nonessential and noninhibitory zinc at 0.5/subunit
Drosophila melanogaster
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.24
Zn2+
the inhibitory zinc is located at a subunit interface using Cys219 and His10 as ligands
Drosophila melanogaster
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.24
0.108
-
5-aminolevulinate
pH 8
Drosophila melanogaster
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.24
Drosophila melanogaster
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.24
-
Drosophila melanogaster
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.24
0.283
-
-
Drosophila melanogaster
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
-
652435
Drosophila melanogaster
porphobilinogen + 2 H2O
-
652435
Drosophila melanogaster
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.24
-
Drosophila melanogaster
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
4.2.1.24
H10F
mutant enzyme is active but is not inhibited by zinc. H10F binds a catalytic zinc at 0.5/subunit and binds a second nonessential and noninhibitory zinc at 0.5/subunit
Drosophila melanogaster
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.24
Zn2+
the inhibitory zinc is located at a subunit interface using Cys219 and His10 as ligands
Drosophila melanogaster
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.24
0.108
-
5-aminolevulinate
pH 8
Drosophila melanogaster
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.24
-
Drosophila melanogaster
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.24
0.283
-
-
Drosophila melanogaster
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
-
652435
Drosophila melanogaster
porphobilinogen + 2 H2O
-
652435
Drosophila melanogaster
?