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Literature summary extracted from

  • Wang, L.K.; Ho, C.K.; Pei, Y.; Shuman, S.
    Mutational analysis of bacteriophage T4 RNA ligase 1. Different functional groups are required for the nucleotidyl transfer and phosphodiester bond formation steps of the ligation reaction (2003), J. Biol. Chem., 278, 29454-29462.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.3 transformed into Escherichia coli BL21 (DE3) Tequatrovirus T4

Protein Variants

EC Number Protein Variants Comment Organism
6.5.1.3 D244A site-directed mutagenesis Tequatrovirus T4
6.5.1.3 E185A site-directed mutagenesis Tequatrovirus T4
6.5.1.3 E227A site-directed mutagenesis Tequatrovirus T4
6.5.1.3 E227D site-directed mutagenesis Tequatrovirus T4
6.5.1.3 E227Q site-directed mutagenesis Tequatrovirus T4
6.5.1.3 E81A site-directed mutagenesis Tequatrovirus T4
6.5.1.3 F76A site-directed mutagenesis Tequatrovirus T4
6.5.1.3 F77A site-directed mutagenesis Tequatrovirus T4
6.5.1.3 F77L site-directed mutagenesis Tequatrovirus T4
6.5.1.3 G102A site-directed mutagenesis Tequatrovirus T4
6.5.1.3 G228A site-directed mutagenesis Tequatrovirus T4
6.5.1.3 G55A site-directed mutagenesis Tequatrovirus T4
6.5.1.3 K119A site-directed mutagenesis Tequatrovirus T4
6.5.1.3 K119Q site-directed mutagenesis Tequatrovirus T4
6.5.1.3 K119R site-directed mutagenesis Tequatrovirus T4
6.5.1.3 K240A site-directed mutagenesis Tequatrovirus T4
6.5.1.3 K240Q site-directed mutagenesis Tequatrovirus T4
6.5.1.3 K240R site-directed mutagenesis Tequatrovirus T4
6.5.1.3 K242A site-directed mutagenesis Tequatrovirus T4
6.5.1.3 K75A site-directed mutagenesis Tequatrovirus T4
6.5.1.3 K75Q site-directed mutagenesis Tequatrovirus T4
6.5.1.3 K75R site-directed mutagenesis Tequatrovirus T4
6.5.1.3 K99A site-directed mutagenesis Tequatrovirus T4
6.5.1.3 N184A site-directed mutagenesis Tequatrovirus T4
6.5.1.3 N78A site-directed mutagenesis Tequatrovirus T4
6.5.1.3 R166A site-directed mutagenesis Tequatrovirus T4
6.5.1.3 R182A site-directed mutagenesis Tequatrovirus T4
6.5.1.3 R54A site-directed mutagenesis Tequatrovirus T4
6.5.1.3 R54K site-directed mutagenesis Tequatrovirus T4
6.5.1.3 R54Q site-directed mutagenesis Tequatrovirus T4
6.5.1.3 R71A site-directed mutagenesis Tequatrovirus T4

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.5.1.3 ATP + (ribonucleotide)n + (ribonucleotide)m Tequatrovirus T4
-
 AMP + diphosphate + (ribonucleotide)n+m
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.3 Tequatrovirus T4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.3 mutant enzymes, expressed in bacteria Tequatrovirus T4

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.3 ATP + (ribonucleotide)n + (ribonucleotide)m
-
 Tequatrovirus T4 AMP + diphosphate + (ribonucleotide)n+m
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.5.1.3 RNA ligase 1
-
 Tequatrovirus T4
6.5.1.3 Rnl1
-
 Tequatrovirus T4