EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.24.B3 | expression of catalytic site comprising residues 98-272 and of mutants A235P and E216A in Escherichia coli | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.4.24.B3 | A235P | mutant shows about 10fold increased activity with alpha1-proteinase inhibitor | Homo sapiens |
3.4.24.B3 | E216A | active site mutant, catalytically inactive | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.B3 | matrix metalloproteinase inhibitor AG3340 | broad spectrum inhibitor | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.24.B3 | 0.148 | - |
GGYAELRMGG | pH 7.5, 37°C, recombinant mutant A235P | Homo sapiens | |
3.4.24.B3 | 0.207 | - |
GGYAELRMGG | pH 7.5, 37°C | Homo sapiens | |
3.4.24.B3 | 0.294 | - |
GGAANLVRGG | pH 7.5, 37°C, recombinant mutant A235P | Homo sapiens | |
3.4.24.B3 | 0.705 | - |
GGAANLVRGG | pH 7.5, 37°C | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.B3 | protein + H2O | Homo sapiens | - |
peptides | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.24.B3 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.24.B3 | recombinant catalytic domain and mutants from Escherichia coli | Homo sapiens |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.4.24.B3 | proteolytic degradation of proteins | reaction and inhibition mechanism | Homo sapiens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.24.B3 | additional information | - |
substrate specificity | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.B3 | alpha1-antitrypsin inhibitor + H2O | - |
Homo sapiens | ? | - |
? | |
3.4.24.B3 | GAAGAMFLEA + H2O | - |
Homo sapiens | GAAGA + MFLEA | - |
? | |
3.4.24.B3 | GGAANLVRGG + H2O | - |
Homo sapiens | GGAAN + LVRGG | - |
? | |
3.4.24.B3 | GGPLALWARGG + H2O | - |
Homo sapiens | GGPLA + LWARGG | - |
? | |
3.4.24.B3 | GGPLGLYAGG + H2O | - |
Homo sapiens | GGPLG + LYAGG | - |
? | |
3.4.24.B3 | GGQPRGVWGG + H2O | - |
Homo sapiens | GGQPRG + VWGG | - |
? | |
3.4.24.B3 | GGTDAWLSGG + H2O | - |
Homo sapiens | GGTDA + WLSGG | - |
? | |
3.4.24.B3 | GGYAELRMGG + H2O | best substrate | Homo sapiens | GGYAE + LRMGG | - |
? | |
3.4.24.B3 | additional information | wild-type enzyme does not cleave extracellular matrix proteins such as collagen, laminin, fibronectin, and elastin | Homo sapiens | ? | - |
? | |
3.4.24.B3 | protein + H2O | - |
Homo sapiens | peptides | - |
? | |
3.4.24.B3 | protein + H2O | substrate specificity, enzyme prefers 2 consensus sequences: 1. A(A/Q)(N/A)_(L/Y)(T/V/M/R)(R/K) and 2. G(G/A)E_LR, with _ marking the cleavage sites, enzyme also cleaves peptides containing Ala in position 3, Ala in position 1, and Leu/Tyr in position 1', peptides with proline in position 3 are poor substrates | Homo sapiens | peptides | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.24.B3 | M10.007 | Merops-ID | Homo sapiens |
3.4.24.B3 | matrix metalloproteinase-11 | - |
Homo sapiens |
3.4.24.B3 | MMP-11 proteinase | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.24.B3 | 37 | - |
assay at | Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.24.B3 | 0.58 | - |
GGYAELRMGG | pH 7.5, 37°C, recombinant wild-type enzyme | Homo sapiens | |
3.4.24.B3 | 0.69 | - |
GGAANLVRGG | pH 7.5, 37°C, recombinant wild-type enzyme | Homo sapiens | |
3.4.24.B3 | 1.65 | - |
GGAANLVRGG | pH 7.5, 37°C, recombinant mutant A235P | Homo sapiens | |
3.4.24.B3 | 3 | 6 | GGYAELRMGG | pH 7.5, 37°C, recombinant mutant A235P | Homo sapiens | |
3.4.24.B3 | 3.08 | - |
GGYAELRMGG | pH 7.5, 37°C, recombinant mutant A235P | Homo sapiens | |
3.4.24.B3 | 6.08 | - |
GGYAELRMGG | pH 7.5, 37°C, recombinant wild-type enzyme | Homo sapiens | |
3.4.24.B3 | 6.08 | - |
GGAANLVRGG | pH 7.5, 37°C, recombinant wild-type enzyme | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.24.B3 | 7.5 | 7.6 | assay at | Homo sapiens |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.24.B3 | 0.000006 | - |
matrix metalloproteinase inhibitor AG3340 | pH 7.6, 37°C | Homo sapiens |