EC Number | Cloned (Comment) | Organism |
---|---|---|
6.2.1.12 | expression in Escherichia coli as His-tag fusion protein | Glycine max |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.2.1.12 | E340A | 4CL1-mutant, activity almost completely abolished | Glycine max |
6.2.1.12 | G333A | 4CL1-mutant, activity almost completely abolished | Glycine max |
6.2.1.12 | G337A | 4CL1-mutant, activity almost completely abolished | Glycine max |
6.2.1.12 | G342A | 4CL1-mutant, activity almost completely abolished | Glycine max |
6.2.1.12 | I346A | 4CL1-mutant, very low activity with all substrates | Glycine max |
6.2.1.12 | L344A | 4CL1-mutant, activity almost completely abolished | Glycine max |
6.2.1.12 | M338A | 4CL1-mutant, activity almost completely abolished | Glycine max |
6.2.1.12 | M348A | 4CL1-mutant, activity almost completely abolished | Glycine max |
6.2.1.12 | additional information | construction of hybrid enzymes using different portions of isoforms 4CL1-4CL3, some of the constructed proteins were found to be inactive | Glycine max |
6.2.1.12 | P285A | 4CL1-mutant, very low activity with all substrates | Glycine max |
6.2.1.12 | P286A | 4CL1-mutant, minor changes in activity | Glycine max |
6.2.1.12 | P343A | 4CL1-mutant, very low activity with all substrates | Glycine max |
6.2.1.12 | Q334A | 4CL1-mutant, very low activity with all substrates | Glycine max |
6.2.1.12 | T331A | 4CL1-mutant, minor changes in activity | Glycine max |
6.2.1.12 | T339A | 4CL1-mutant, activity almost completely abolished | Glycine max |
6.2.1.12 | V284A | 4CL1-mutant, minor changes in activity | Glycine max |
6.2.1.12 | Y336A | 4CL1-mutant, activity almost completely abolished | Glycine max |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.2.1.12 | 0.004 | - |
ferulate | 4CL1 | Glycine max | |
6.2.1.12 | 0.005 | - |
sinapate | 4CL1 | Glycine max | |
6.2.1.12 | 0.009 | - |
4-coumarate | 4CL3 | Glycine max | |
6.2.1.12 | 0.013 | - |
caffeate | 4CL2 | Glycine max | |
6.2.1.12 | 0.042 | - |
4-coumarate | 4CL2 | Glycine max | |
6.2.1.12 | 0.049 | - |
3,4-dimethoxycinnamate | 4CL1 | Glycine max | |
6.2.1.12 | 0.05 | - |
4-coumarate | 4CL1 | Glycine max | |
6.2.1.12 | 0.05 | - |
caffeate | 4CL3 | Glycine max | |
6.2.1.12 | 0.081 | - |
caffeate | 4CL1 | Glycine max | |
6.2.1.12 | 0.14 | - |
ferulate | 4CL2 | Glycine max | |
6.2.1.12 | 1.1 | - |
cinnamate | 4CL3 | Glycine max | |
6.2.1.12 | 1.7 | - |
cinnamate | 4CL2 | Glycine max | |
6.2.1.12 | 3.1 | - |
ferulate | 4CL3 | Glycine max | |
6.2.1.12 | 4.4 | - |
cinnamate | 4CL1 | Glycine max |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.2.1.12 | ATP + 4-coumarate + CoA | Glycine max | plant phenylpropanoid metabolism | AMP + diphosphate + 4-coumaroyl-CoA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.2.1.12 | Glycine max | - |
isoforms 4CL1-4CL3 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.2.1.12 | recombinant protein using His-tag | Glycine max |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.2.1.12 | ATP + 3,4-dimethoxycinnamate + CoA | substrate for 4CL1 but not for 4CL2 and 4CL3 | Glycine max | AMP + diphosphate + 3,4-dimethoxycinnamoyl-CoA | - |
? | |
6.2.1.12 | ATP + 4-coumarate + CoA | - |
Glycine max | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
6.2.1.12 | ATP + 4-coumarate + CoA | plant phenylpropanoid metabolism | Glycine max | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
6.2.1.12 | ATP + caffeate + CoA | - |
Glycine max | AMP + diphosphate + caffeoyl-CoA | - |
? | |
6.2.1.12 | ATP + cinnamate + CoA | - |
Glycine max | AMP + diphosphate + cinnamoyl-CoA | - |
? | |
6.2.1.12 | ATP + ferulate + CoA | substrate for 4CL1 and 4CL2, very poor substrate for 4CL3 | Glycine max | AMP + diphosphate + feruloyl-CoA | - |
? | |
6.2.1.12 | ATP + sinapate + CoA | substrate for 4CL1 but not for 4CL2 and 4CL3 | Glycine max | AMP + diphosphate + sinapoyl-CoA | - |
? |