EC Number | Cloned (Comment) | Organism |
---|---|---|
6.3.4.16 | expression in Saccharomyces pombe | Lithobates catesbeianus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.3.4.16 | E261Q | no synthesis of carbonyl phosphate in presence of L-glutamine | Lithobates catesbeianus |
6.3.4.16 | K258L | synthesis of carbonyl phosphate in presence of L-glutamine | Lithobates catesbeianus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.4.16 | 0.27 | - |
ATP | wild-type enzyme, comparison to native and mutant enzyme | Lithobates catesbeianus | |
6.3.4.16 | 0.39 | - |
ammonia | wild-type enzyme, comparison to native and mutant enzyme | Lithobates catesbeianus | |
6.3.5.5 | 0.05 | - |
ATP | glutamine-dependent ADP formation | Escherichia coli | |
6.3.5.5 | 0.06 | - |
ATP | ammonia-dependent ADP formation | Escherichia coli | |
6.3.5.5 | 0.17 | - |
L-glutamine | - |
Escherichia coli | |
6.3.5.5 | 111 | - |
ammonia | - |
Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.4.16 | 2 ATP + NH4+ + HCO3- | Homo sapiens | - |
2 ADP + phosphate + carbamoyl phosphate | - |
? | |
6.3.4.16 | 2 ATP + NH4+ + HCO3- | Rattus norvegicus | - |
2 ADP + phosphate + carbamoyl phosphate | - |
? | |
6.3.4.16 | 2 ATP + NH4+ + HCO3- | Lithobates catesbeianus | - |
2 ADP + phosphate + carbamoyl phosphate | - |
? | |
6.3.5.5 | 2 ATP + L-Gln + HCO3- | Escherichia coli | the product carbamoyl phosphate is utilized for the arginine biosynthesis | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.4.16 | Homo sapiens | - |
- |
- |
6.3.4.16 | Lithobates catesbeianus | - |
- |
- |
6.3.4.16 | Rattus norvegicus | - |
- |
- |
6.3.5.5 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.3.4.16 | - |
Lithobates catesbeianus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
6.3.4.16 | 2 ATP + NH3 + hydrogencarbonate = 2 ADP + phosphate + carbamoyl phosphate | depending on their physiological role, carbonyl phosphate synthetases use either glutamine or free ammonia as the nitrogen donor for carbamoyl phosphate synthesis, all enzymes contain the structurally equivalent of a triad-type glutamine amidotransferase, GAT, domain | Lithobates catesbeianus | |
6.3.4.16 | 2 ATP + NH3 + hydrogencarbonate = 2 ADP + phosphate + carbamoyl phosphate | depending on their physiological role, carbonyl phosphate synthetases uses either glutamine or free ammonia as the nitrogen donor for carbamoyl phosphate synthesis, all enzymes contain the structurell equivalent of a triad-type glutamine amidotransferase, GAT, domain | Homo sapiens | |
6.3.4.16 | 2 ATP + NH3 + hydrogencarbonate = 2 ADP + phosphate + carbamoyl phosphate | depending on their physiological role, carbonyl phosphate synthetasese uses either glutamine or free ammonia as the nitrogen donor for carbamoyl phosphate synthesis, all enzymes contain the structurell equivalent of a triad-type glutamine amidotransferase, GAT, domain | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.4.16 | 2 ATP + NH4+ + HCO3- | - |
Homo sapiens | 2 ADP + phosphate + carbamoyl phosphate | - |
? | |
6.3.4.16 | 2 ATP + NH4+ + HCO3- | - |
Rattus norvegicus | 2 ADP + phosphate + carbamoyl phosphate | - |
? | |
6.3.4.16 | 2 ATP + NH4+ + HCO3- | - |
Lithobates catesbeianus | 2 ADP + phosphate + carbamoyl phosphate | - |
? | |
6.3.5.5 | 2 ATP + L-Gln + HCO3- | - |
Escherichia coli | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? | |
6.3.5.5 | 2 ATP + L-Gln + HCO3- | the product carbamoyl phosphate is utilized for the arginine biosynthesis | Escherichia coli | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? | |
6.3.5.5 | 2 ATP + NH4+ + HCO3- | - |
Escherichia coli | 2 ADP + phosphate + carbamoyl phosphate | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.4.16 | 1.8 | - |
ammonia | wild-type enzyme, comparison to native and mutant enzyme | Lithobates catesbeianus | |
6.3.4.16 | 2.4 | - |
ATP | wild-type enzyme, comparison to native and mutant enzyme | Lithobates catesbeianus | |
6.3.5.5 | 2.2 | - |
ATP | ammonia-dependent ADP formation | Escherichia coli | |
6.3.5.5 | 5.1 | - |
ammonia | - |
Escherichia coli | |
6.3.5.5 | 6.8 | - |
ATP | glutamine-dependent ADP formation | Escherichia coli | |
6.3.5.5 | 9.4 | - |
L-glutamine | - |
Escherichia coli |