Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Goto, M.; Omi, R.; Miyahara, I.; Sugahara, M.; Hirotsu, K.
    Structures of argininosuccinate synthetase in enzyme-ATP substrates and enzyme-AMP product forms. Stereochemistry of the catalytic reaction (2003), J. Biol. Chem., 278, 22964-22971.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.5 expression in Escherichia coli BL21(DE3)pLysE Thermus thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.4.5 structures of enzyme complexed with intact ATP and substrates citrulline and aspartate, complexed with AMP and argininosuccinate, and complexed with AMP-PNP, arginine, aspartate and Mg2+, vapor diffusion method, X-ray analysis Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.5 Mg2+ required for maximal activity, in octahedral coordination with 3 oxygen atoms of the triphosphate group and 3 water molecules Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.5 44815
-
 4 * 44815, recombinant tAsS, subunit/domain structure, amino acid sequence Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.5 ATP + L-citrulline + L-aspartate Thermus thermophilus seventh step of the arginine biosynthesis, second step of the urea cycle AMP + diphosphate + L-argininosuccinate
-
 ?
6.3.4.5 ATP + L-citrulline + L-aspartate Thermus thermophilus HB8 / ATCC 27634 / DSM 579 seventh step of the arginine biosynthesis, second step of the urea cycle AMP + diphosphate + L-argininosuccinate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.5 Thermus thermophilus P59846
-
-
6.3.4.5 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 P59846
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.5
-
 Thermus thermophilus

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.4.5 ATP + L-citrulline + L-aspartate = AMP + diphosphate + 2-(Nomega-L-arginino)succinate detailed catalytic mechanism, stereochemistry Thermus thermophilus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.4.5 0.06
-
 20°C Thermus thermophilus
6.3.4.5 0.92
-
 70°C Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.5 ATP + L-citrulline + L-aspartate ATP-dependent condensation of citrulline with aspartate, detailed mechanism via citrullyl-AMP intermediate, stereochemistry of catalysis, no conformational change upon binding of ATP, active site structure Thermus thermophilus AMP + diphosphate + L-argininosuccinate
-
 r
6.3.4.5 ATP + L-citrulline + L-aspartate seventh step of the arginine biosynthesis, second step of the urea cycle Thermus thermophilus AMP + diphosphate + L-argininosuccinate
-
 ?
6.3.4.5 ATP + L-citrulline + L-aspartate ATP-dependent condensation of citrulline with aspartate, detailed mechanism via citrullyl-AMP intermediate, stereochemistry of catalysis, no conformational change upon binding of ATP, active site structure Thermus thermophilus HB8 / ATCC 27634 / DSM 579 AMP + diphosphate + L-argininosuccinate
-
 r
6.3.4.5 ATP + L-citrulline + L-aspartate seventh step of the arginine biosynthesis, second step of the urea cycle Thermus thermophilus HB8 / ATCC 27634 / DSM 579 AMP + diphosphate + L-argininosuccinate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.5 tetramer 4 * 44815, recombinant tAsS, subunit/domain structure, amino acid sequence Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
6.3.4.5 Argininosuccinate synthetase
-
 Thermus thermophilus
6.3.4.5 ASS
-
 Thermus thermophilus
6.3.4.5 tAsS
-
 Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.4.5 8.5
-
 assay at Thermus thermophilus