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Literature summary extracted from

  • Jackson, L.K.; Goldsmith, E.J.; Phillips, M.A.
    X-ray structure determination of Trypanosoma brucei ornithine decarboxylase bound to D-ornithine and to G418: insights into substrate binding and ODC conformational flexibility (2003), J. Biol. Chem., 278, 22037-22043.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.17 co-crystallization with D-ornithine and geneticin sulfate, 23 mg/ml ODC in 10 mM HEPES, pH 7.2, 50 mM NaCl, 10 mM dithiothreitol, 0.5 mM EDTA, 0.01% Brij-20, is preincubated with 25 mM D-ornithine and 100 mM geneticin sulfate for 10 min at room temperature, equal volumes of preincubated enzyme and well solution consisting of 20% polyethylene glycol 3350, 100 mM HEPES, pH 8.0, 10 mM dithiothreitol, 25 mM D-ornithine, pH 7.5, and 100 mM geneticine are used to form crystal drops, crystals diffract to 2.5 A Trypanosoma brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.17 geneticin G418, weak non-competitive inhibition cs. L-ornithine Trypanosoma brucei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.17 L-ornithine Trypanosoma brucei first step in polyamine biosynthesis putrescine + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.17 Trypanosoma brucei
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.17 L-ornithine
-
 Trypanosoma brucei putrescine + CO2
-
 ?
4.1.1.17 L-ornithine first step in polyamine biosynthesis Trypanosoma brucei putrescine + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.17 ODC
-
 Trypanosoma brucei

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.17 pyridoxal 5'-phosphate
-
 Trypanosoma brucei

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.1.17 4.5
-
 geneticin pH 8.0 Trypanosoma brucei
4.1.1.17 8
-
 geneticin pH 7.0 Trypanosoma brucei