EC Number | Application | Comment | Organism |
---|---|---|---|
3.1.1.6 | analysis | development of a heroin biosensor with high sensitivity | Rhodococcus sp. |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.6 | expression of the N-terminally His-tagged enzyme in Escherichia coli BL21(DE3) | Rhodococcus sp. |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.1.1.6 | purified selenomethionine enzyme, 6-7 mg/ml, pure or complexed with the inhibitor dimethylarsinic acid, crystal growth in 1.8 M ammonium sulfate, 0.1 M NaCl, 0.1 M cacodylate, pH 6.5, for crystallization of native methionine enzyme 1.7 M ammonium sulfate, 0.1 M NaCl, 0.1 M BES, pH 6.4, is used, X-ray structure determination and analysis at 1.3 A and 1.45 A resolution | Rhodococcus sp. |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.1.6 | additional information | construction of selenomethionine enzyme by growth of the recombinant methionine auxotroph Escherichia coli strain B834-(DE3) expressing the enzyme in minimal medium containing selenomethionine | Rhodococcus sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.6 | dimethylarsinic acid | binding mechanism | Rhodococcus sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.6 | 0.07 | - |
phenyl acetate | recombinant enzyme, pH 6.4 | Rhodococcus sp. | |
3.1.1.6 | 0.5 | - |
6-acetylmorphine | recombinant enzyme, pH 8.0 | Rhodococcus sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.6 | Rhodococcus sp. | - |
- |
- |
3.1.1.6 | Rhodococcus sp. H1 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.6 | recombinant from Escherichia coli as N-terminally His-tagged selenomethionine or methionine enzyme, removal of the His-tag | Rhodococcus sp. |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.1.1.6 | an acetic ester + H2O = an alcohol + acetate | active site serine, active site structure, reaction and ligand binding mechanism | Rhodococcus sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.6 | 3,6-diacetylmorphine + H2O | i.e. heroin, rapid spontaneous hydrolysis of the instable 3-acetyl group | Rhodococcus sp. | morphine + acetate | - |
? | |
3.1.1.6 | 3,6-diacetylmorphine + H2O | i.e. heroin, rapid spontaneous hydrolysis of the instable 3-acetyl group | Rhodococcus sp. H1 | morphine + acetate | - |
? | |
3.1.1.6 | 6-acetylmorphine + H2O | substrate specificity | Rhodococcus sp. | morphine + acetate | - |
? | |
3.1.1.6 | 6-acetylmorphine + H2O | substrate specificity | Rhodococcus sp. H1 | morphine + acetate | - |
? | |
3.1.1.6 | phenyl acetate + H2O | - |
Rhodococcus sp. | phenol + acetate | - |
? | |
3.1.1.6 | phenyl acetate + H2O | - |
Rhodococcus sp. H1 | phenol + acetate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.6 | HerE | - |
Rhodococcus sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.6 | 3 | - |
phenyl acetate | recombinant enzyme, pH 6.4 | Rhodococcus sp. | |
3.1.1.6 | 12.6 | - |
6-acetylmorphine | recombinant enzyme, pH 8.0 | Rhodococcus sp. |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.6 | 12 | - |
dimethylarsinic acid | recombinant enzyme, pH 6.4 | Rhodococcus sp. |