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Literature summary extracted from

  • Wu, J.; Woodard, R.W.
    Escherichia coli YrbI is 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (2003), J. Biol. Chem., 278, 18117-18123.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.45 Ca2+
-
 Escherichia coli
3.1.3.45 Cd2+
-
 Escherichia coli
3.1.3.45 Cu2+
-
 Escherichia coli
3.1.3.45 Hg2+
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.45 0.075
-
 3-deoxy-D-manno-octulosonate 8-phosphate pH 7.0, 37°C, presence of 1 mM Mg2+ Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.45 Ba2+ 1 mM, little stimulation Escherichia coli
3.1.3.45 Co2+ 1 mM, stimulation about 9fold Escherichia coli
3.1.3.45 Mg2+ 1 mM, stimulation about 9fold Escherichia coli
3.1.3.45 Mn2+ 1 mM, little stimulation Escherichia coli
3.1.3.45 additional information divalent metal cofactor required Escherichia coli
3.1.3.45 Zn2+ 1 mM, little stimulation Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.45 19900
-
 4 * 23000, SDS-PAGE, 4 * 19900, mass spectrometry Escherichia coli
3.1.3.45 23000
-
 4 * 23000, SDS-PAGE, 4 * 19900, mass spectrometry Escherichia coli
3.1.3.45 89000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.45 additional information Escherichia coli last enzyme of 3-deoxy-D-manno-octulosonate biosynthetic pathway ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.45 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.45
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.45 3-deoxy-D-manno-octulosonate 8-phosphate + H2O high specificity Escherichia coli 3-deoxy-D-manno-octulosonate + phosphate
-
 ?
3.1.3.45 additional information last enzyme of 3-deoxy-D-manno-octulosonate biosynthetic pathway Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.45 tetramer 4 * 23000, SDS-PAGE, 4 * 19900, mass spectrometry Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.45 5.5
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.45 5.5 7
-
 Escherichia coli

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.3.45 Escherichia coli isoelectric focusing 4.8 4.7