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Literature summary extracted from

  • Pezza, J.A.; Choi, K.H.; Berardini, T.Z.; Beernink, P.T.; Allen, K.N.; Tolan, D.R.
    Spatial clustering of isozyme-specific residues reveals unlikely determinants of isozyme specificity in fructose-1,6-bisphosphate aldolase (2003), J. Biol. Chem., 278, 17307-17313.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.13
-
 Oryctolagus cuniculus

Protein Variants

EC Number Protein Variants Comment Organism
4.1.2.13 H156E similar kinetics and stability as wild-type enzyme Oryctolagus cuniculus
4.1.2.13 additional information AB_All mutant, isozyme-specific residues from aldolase B swapped into aldolase A and vice versa, decreased Km and kcat compared to aldolase A, similar Km and kcat to aldolase B Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.13 0.00084
-
 D-fructose 1,6-bisphosphate 30°C, aldolase B Oryctolagus cuniculus
4.1.2.13 0.0011
-
 D-fructose 1,6-bisphosphate 30°C, AB_All mutant Oryctolagus cuniculus
4.1.2.13 0.0095
-
 D-fructose 1,6-bisphosphate 30°C, aldolase A Oryctolagus cuniculus
4.1.2.13 0.0102
-
 D-fructose 1,6-bisphosphate 30°C, H156E mutant Oryctolagus cuniculus
4.1.2.13 0.723
-
 D-Fructose 1-phosphate 30°C, aldolase B Oryctolagus cuniculus
4.1.2.13 2.6
-
 D-Fructose 1-phosphate 30°C, AB_All mutant Oryctolagus cuniculus
4.1.2.13 39.8
-
 D-Fructose 1-phosphate 30°C, aldolase A Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.13 D-fructose 1,6-bisphosphate Oryctolagus cuniculus
-
 glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.13 Oryctolagus cuniculus P00883 rabbit
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.13
-
 Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.13 D-fructose 1,6-bisphosphate
-
 Oryctolagus cuniculus glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 ?
4.1.2.13 D-Fructose 1-phosphate
-
 Oryctolagus cuniculus Glycerone phosphate + D-glyceraldehyde
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.2.13 ClassII FBP-aldolase
-
 Oryctolagus cuniculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.13 0.63
-
 D-Fructose 1-phosphate 30°C, aldolase A Oryctolagus cuniculus
4.1.2.13 1.07
-
 D-fructose 1,6-bisphosphate 30°C, AB_All mutant Oryctolagus cuniculus
4.1.2.13 1.47
-
 D-Fructose 1-phosphate 30°C, AB_All mutant Oryctolagus cuniculus
4.1.2.13 1.53
-
 D-fructose 1,6-bisphosphate 30°C, aldolase B Oryctolagus cuniculus
4.1.2.13 2.94
-
 D-fructose 1,6-bisphosphate 30°C, aldolase B Oryctolagus cuniculus
4.1.2.13 6.08
-
 D-fructose 1,6-bisphosphate 30°C, AB_All mutant Oryctolagus cuniculus
4.1.2.13 6.08
-
 D-Fructose 1-phosphate 30°C, AB_All mutant Oryctolagus cuniculus
4.1.2.13 6.08
-
 D-Fructose 1-phosphate 30°C, aldolase A Oryctolagus cuniculus
4.1.2.13 6.08
-
 D-Fructose 1-phosphate 30°C, aldolase B Oryctolagus cuniculus
4.1.2.13 10.2
-
 D-fructose 1,6-bisphosphate 30°C, aldolase A Oryctolagus cuniculus
4.1.2.13 16.5
-
 D-fructose 1,6-bisphosphate 30°C, H156E mutant Oryctolagus cuniculus