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Literature summary extracted from
- Isochorismate synthase (PchA), the first and rate-limiting enzyme in salicylate biosynthesis of Pseudomonas aeruginosa (2003), J. Biol. Chem., 278, 16893-16898.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.4.2 | 0.0045 | - |
chorismate | - |
Pseudomonas aeruginosa |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.4.2 | Mg2+ | strictly required for activity | Pseudomonas aeruginosa | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.4.4.2 | 48000 | 50000 | gel filtration | Pseudomonas aeruginosa |
| 5.4.4.2 | 50000 | - |
native PAGE | Pseudomonas aeruginosa |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.4.2 | Chorismate | Pseudomonas aeruginosa | first enzyme of pyochelin biosynthesis | Isochorismate | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.4.2 | Pseudomonas aeruginosa | Q51508 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.4.2 | DEAE-Sepharose, Phenyl-Sepharose, Mono Q | Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.4.4.2 | 0.0017 | - |
- |
Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.4.2 | Chorismate | - |
Pseudomonas aeruginosa | Isochorismate | - |
r | |
| 5.4.4.2 | Chorismate | first enzyme of pyochelin biosynthesis | Pseudomonas aeruginosa | Isochorismate | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.4.4.2 | monomer | 1 * 50000, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.4.2 | PchA | - |
Pseudomonas aeruginosa |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.4.2 | 0.72 | - |
chorismate | - |
Pseudomonas aeruginosa | |
| 5.4.4.2 | 6.08 | - |
chorismate | - |
Pseudomonas aeruginosa | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.4.2 | 7 | - |
- |
Pseudomonas aeruginosa |
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