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Literature summary extracted from

  • Dziembowski, A.; Piwowarski, J.; Hoser, R.; Minczuk, M.; Dmochowska, A.; Siep, M.; van der Spek, H.; Grivell, L.; Stepien, P.P.
    The yeast degradosome. Its composition, interplay between RNA helicase and RNase activities and the role in mitochondrial RNA metabolism (2003), J. Biol. Chem., 278, 1603-1611.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.14.1 expression of wild-type and mutants in different yeast strains Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
3.1.14.1 Y814S point mutation in Dssp1, mutant strain is respiratory incompetent with very unstable mitochondrial genomes Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.14.1 mitochondrion
-
 Saccharomyces cerevisiae 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.14.1 Mg2+
-
 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.14.1 additional information Saccharomyces cerevisiae enzyme is part of the RNA degrading enzyme complex in mitochondria, the degradosome, which is a central part of a mitochondrial RNA surveillance system responsible for degradation of aberrant and unprocessed RNAs ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.14.1 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.14.1 recombinant degradosome from Saccharomyces cerevisiae W303 strain derivatives Saccharomyces cerevisiae

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.14.1 exonucleolytic cleavage to nucleoside 3'-phosphates conserved Tyr814 is involved in catalysis and is essential for enzyme Dssp1 function and mitochondrial biogenesis Saccharomyces cerevisiae
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.14.1 additional information enzyme is part of the RNA degrading enzyme complex, the degradosome, in mitochondria, consisting of a RNA helicase and a RNase activity, the former being essential for exoribonuclease activity of the complex, the degradosome is not involved in tRNA processing Saccharomyces cerevisiae ?
-
 ?
3.1.14.1 additional information enzyme is part of the RNA degrading enzyme complex in mitochondria, the degradosome, which is a central part of a mitochondrial RNA surveillance system responsible for degradation of aberrant and unprocessed RNAs Saccharomyces cerevisiae ?
-
 ?
3.1.14.1 RNA + H2O mitochondrial mRNA and rRNA Saccharomyces cerevisiae nucleoside 3'-phosphates
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.14.1 More enzyme is part of the RNA degrading enzyme complex, the degradosome, in mitochondria, consisting of 2 large subunits Dssp1 and Suv3p, a RNA helicase and a RNase activity, the former being essential for exoribonuclease activity of the complex Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
3.1.14.1 mtEXO
-
 Saccharomyces cerevisiae
3.1.14.1 yeast mitochondrial degradosome
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.14.1 30
-
 RNA helicase assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.14.1 8
-
 RNA helicase assay at Saccharomyces cerevisiae