EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.25.1 | leupeptin | IC50: 0.0015 mM, inhibition of hydrolysis of Gly-Gly-Arg-7-amido-4-methylcoumarin, most potent inhibitor for hydrolysis of Gly-Gly-Arg-7-amido-4-methylcoumarin. IC50: 0.1 mM, inhibition of hydrolysis of succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin | Trypanosoma brucei | |
3.4.25.1 | NPLLLVS | IC50: 0.04 mM, inhibition of hydrolysis of Gly-Gly-Arg-7-amido-4-methylcoumarin. IC50: 0.007 mM, inhibition of hydrolysis of succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin, most potent inhibitor for hydrolysis of succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin | Trypanosoma brucei | |
3.4.25.1 | NPLLNVS | IC50: 0.063 mM, inhibition of hydrolysis of Gly-Gly-Arg-7-amido-4-carbamoylcoumarin. IC50: 0.077 mM, inhibition of hydrolysis of succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin | Trypanosoma brucei | |
3.4.25.1 | YLLLVS | IC50: 0.048 mM, inhibition of hydrolysis of Gly-Gly-Arg-7-amido-4-methylcoumarin. IC50: 0.033 mM, inhibition of hydrolysis of succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin | Trypanosoma brucei |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.25.1 | 0.027 | - |
acetyl-YWTQ-7-amido-4-carbamoylcoumarin | pH 7.8, 25°C | Homo sapiens | |
3.4.25.1 | 0.032 | - |
acetyl-EPFD-7-amido-4-carbamoylcoumarin | pH 7.8, 25°C | Homo sapiens | |
3.4.25.1 | 0.038 | - |
acetyl-YWTQ-7-amido-4-carbamoylcoumarin | pH 7.8, 25°C | Trypanosoma brucei | |
3.4.25.1 | 0.088 | - |
acetyl-EPFD-7-amido-4-carbamoylcoumarin | pH 7.8, 25°C | Trypanosoma brucei | |
3.4.25.1 | 0.11 | - |
acetyl-norleucine-Arg-norleucine-Arg-7-amido-4-carbamoylcoumarin | pH 7.8, 25°C | Homo sapiens | |
3.4.25.1 | 0.36 | - |
acetyl-HHSL-7-amido-4-carbamoylcoumarin | pH 7.5, 25°C | Homo sapiens | |
3.4.25.1 | 0.67 | - |
acetyl-norleucine-Arg-norleucine-Arg-7-amido-4-carbamoylcoumarin | pH 7.8, 25°C | Trypanosoma brucei |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.25.1 | 22700 | - |
1 * 28000, alpha1, + 1 * 24300, alpha2, + 1 * 33000, alpha3, + 1 * 27000, alpha4, + 1 * 27500, alpha5, + 1 * 29000, alpha6, + 1 * 25000, alpha7, + 1 * 24700, beta1, + 1 * 24000, beta2, + 1 * 23200, beta3, + 1 * 23800, beta4, + 1 * 22700, beta5, + 1 * 24500, beta6, + 1 * 24000, beta7, the 20S proteasome is made up of seven alpha-subunits and seven beta-subunits. Of the seven beta-type subunits, five contain pro-sequences that are proteolytically removed during assembly, and three of them are predicted to be catalytic based on primary sequence | Trypanosoma brucei |
3.4.25.1 | 23200 | - |
1 * 28000, alpha1, + 1 * 24300, alpha2, + 1 * 33000, alpha3, + 1 * 27000, alpha4, + 1 * 27500, alpha5, + 1 * 29000, alpha6, + 1 * 25000, alpha7, + 1 * 24700, beta1, + 1 * 24000, beta2, + 1 * 23200, beta3, + 1 * 23800, beta4, + 1 * 22700, beta5, + 1 * 24500, beta6, + 1 * 24000, beta7, the 20S proteasome is made up of seven alpha-subunits and seven beta-subunits. Of the seven beta-type subunits, five contain pro-sequences that are proteolytically removed during assembly, and three of them are predicted to be catalytic based on primary sequence | Trypanosoma brucei |
3.4.25.1 | 23800 | - |
1 * 28000, alpha1, + 1 * 24300, alpha2, + 1 * 33000, alpha3, + 1 * 27000, alpha4, + 1 * 27500, alpha5, + 1 * 29000, alpha6, + 1 * 25000, alpha7, + 1 * 24700, beta1, + 1 * 24000, beta2, + 1 * 23200, beta3, + 1 * 23800, beta4, + 1 * 22700, beta5, + 1 * 24500, beta6, + 1 * 24000, beta7, the 20S proteasome is made up of seven alpha-subunits and seven beta-subunits. Of the seven beta-type subunits, five contain pro-sequences that are proteolytically removed during assembly, and three of them are predicted to be catalytic based on primary sequence | Trypanosoma brucei |
3.4.25.1 | 24000 | - |
1 * 28000, alpha1, + 1 * 24300, alpha2, + 1 * 33000, alpha3, + 1 * 27000, alpha4, + 1 * 27500, alpha5, + 1 * 29000, alpha6, + 1 * 25000, alpha7, + 1 * 24700, beta1, + 1 * 24000, beta2, + 1 * 23200, beta3, + 1 * 23800, beta4, + 1 * 22700, beta5, + 1 * 24500, beta6, + 1 * 24000, beta7, the 20S proteasome is made up of seven alpha-subunits and seven beta-subunits. Of the seven beta-type subunits, five contain pro-sequences that are proteolytically removed during assembly, and three of them are predicted to be catalytic based on primary sequence | Trypanosoma brucei |
3.4.25.1 | 24300 | - |
1 * 28000, alpha1, + 1 * 24300, alpha2, + 1 * 33000, alpha3, + 1 * 27000, alpha4, + 1 * 27500, alpha5, + 1 * 29000, alpha6, + 1 * 25000, alpha7, + 1 * 24700, beta1, + 1 * 24000, beta2, + 1 * 23200, beta3, + 1 * 23800, beta4, + 1 * 22700, beta5, + 1 * 24500, beta6, + 1 * 24000, beta7, the 20S proteasome is made up of seven alpha-subunits and seven beta-subunits. Of the seven beta-type subunits, five contain pro-sequences that are proteolytically removed during assembly, and three of them are predicted to be catalytic based on primary sequence | Trypanosoma brucei |
3.4.25.1 | 24500 | - |
1 * 28000, alpha1, + 1 * 24300, alpha2, + 1 * 33000, alpha3, + 1 * 27000, alpha4, + 1 * 27500, alpha5, + 1 * 29000, alpha6, + 1 * 25000, alpha7, + 1 * 24700, beta1, + 1 * 24000, beta2, + 1 * 23200, beta3, + 1 * 23800, beta4, + 1 * 22700, beta5, + 1 * 24500, beta6, + 1 * 24000, beta7, the 20S proteasome is made up of seven alpha-subunits and seven beta-subunits. Of the seven beta-type subunits, five contain pro-sequences that are proteolytically removed during assembly, and three of them are predicted to be catalytic based on primary sequence | Trypanosoma brucei |
3.4.25.1 | 24700 | - |
1 * 28000, alpha1, + 1 * 24300, alpha2, + 1 * 33000, alpha3, + 1 * 27000, alpha4, + 1 * 27500, alpha5, + 1 * 29000, alpha6, + 1 * 25000, alpha7, + 1 * 24700, beta1, + 1 * 24000, beta2, + 1 * 23200, beta3, + 1 * 23800, beta4, + 1 * 22700, beta5, + 1 * 24500, beta6, + 1 * 24000, beta7, the 20S proteasome is made up of seven alpha-subunits and seven beta-subunits. Of the seven beta-type subunits, five contain pro-sequences that are proteolytically removed during assembly, and three of them are predicted to be catalytic based on primary sequence | Trypanosoma brucei |
3.4.25.1 | 25000 | - |
1 * 28000, alpha1, + 1 * 24300, alpha2, + 1 * 33000, alpha3, + 1 * 27000, alpha4, + 1 * 27500, alpha5, + 1 * 29000, alpha6, + 1 * 25000, alpha7, + 1 * 24700, beta1, + 1 * 24000, beta2, + 1 * 23200, beta3, + 1 * 23800, beta4, + 1 * 22700, beta5, + 1 * 24500, beta6, + 1 * 24000, beta7, the 20S proteasome is made up of seven alpha-subunits and seven beta-subunits. Of the seven beta-type subunits, five contain pro-sequences that are proteolytically removed during assembly, and three of them are predicted to be catalytic based on primary sequence | Trypanosoma brucei |
3.4.25.1 | 27000 | - |
1 * 28000, alpha1, + 1 * 24300, alpha2, + 1 * 33000, alpha3, + 1 * 27000, alpha4, + 1 * 27500, alpha5, + 1 * 29000, alpha6, + 1 * 25000, alpha7, + 1 * 24700, beta1, + 1 * 24000, beta2, + 1 * 23200, beta3, + 1 * 23800, beta4, + 1 * 22700, beta5, + 1 * 24500, beta6, + 1 * 24000, beta7, the 20S proteasome is made up of seven alpha-subunits and seven beta-subunits. Of the seven beta-type subunits, five contain pro-sequences that are proteolytically removed during assembly, and three of them are predicted to be catalytic based on primary sequence | Trypanosoma brucei |
3.4.25.1 | 27500 | - |
1 * 28000, alpha1, + 1 * 24300, alpha2, + 1 * 33000, alpha3, + 1 * 27000, alpha4, + 1 * 27500, alpha5, + 1 * 29000, alpha6, + 1 * 25000, alpha7, + 1 * 24700, beta1, + 1 * 24000, beta2, + 1 * 23200, beta3, + 1 * 23800, beta4, + 1 * 22700, beta5, + 1 * 24500, beta6, + 1 * 24000, beta7, the 20S proteasome is made up of seven alpha-subunits and seven beta-subunits. Of the seven beta-type subunits, five contain pro-sequences that are proteolytically removed during assembly, and three of them are predicted to be catalytic based on primary sequence | Trypanosoma brucei |
3.4.25.1 | 28000 | - |
1 * 28000, alpha1, + 1 * 24300, alpha2, + 1 * 33000, alpha3, + 1 * 27000, alpha4, + 1 * 27500, alpha5, + 1 * 29000, alpha6, + 1 * 25000, alpha7, + 1 * 24700, beta1, + 1 * 24000, beta2, + 1 * 23200, beta3, + 1 * 23800, beta4, + 1 * 22700, beta5, + 1 * 24500, beta6, + 1 * 24000, beta7, the 20S proteasome is made up of seven alpha-subunits and seven beta-subunits. Of the seven beta-type subunits, five contain pro-sequences that are proteolytically removed during assembly, and three of them are predicted to be catalytic based on primary sequence | Trypanosoma brucei |
3.4.25.1 | 29000 | - |
1 * 28000, alpha1, + 1 * 24300, alpha2, + 1 * 33000, alpha3, + 1 * 27000, alpha4, + 1 * 27500, alpha5, + 1 * 29000, alpha6, + 1 * 25000, alpha7, + 1 * 24700, beta1, + 1 * 24000, beta2, + 1 * 23200, beta3, + 1 * 23800, beta4, + 1 * 22700, beta5, + 1 * 24500, beta6, + 1 * 24000, beta7, the 20S proteasome is made up of seven alpha-subunits and seven beta-subunits. Of the seven beta-type subunits, five contain pro-sequences that are proteolytically removed during assembly, and three of them are predicted to be catalytic based on primary sequence | Trypanosoma brucei |
3.4.25.1 | 33000 | - |
1 * 28000, alpha1, + 1 * 24300, alpha2, + 1 * 33000, alpha3, + 1 * 27000, alpha4, + 1 * 27500, alpha5, + 1 * 29000, alpha6, + 1 * 25000, alpha7, + 1 * 24700, beta1, + 1 * 24000, beta2, + 1 * 23200, beta3, + 1 * 23800, beta4, + 1 * 22700, beta5, + 1 * 24500, beta6, + 1 * 24000, beta7, the 20S proteasome is made up of seven alpha-subunits and seven beta-subunits. Of the seven beta-type subunits, five contain pro-sequences that are proteolytically removed during assembly, and three of them are predicted to be catalytic based on primary sequence | Trypanosoma brucei |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.25.1 | Homo sapiens | - |
- |
- |
3.4.25.1 | Trypanosoma brucei | O96780 | beta2 subunit | - |
3.4.25.1 | Trypanosoma brucei | O96787 | beta1 subunit | - |
3.4.25.1 | Trypanosoma brucei | O96788 | alpha6 subunit | - |
3.4.25.1 | Trypanosoma brucei | Q9BMX8 | beta7 subunit | - |
3.4.25.1 | Trypanosoma brucei | Q9GU36 | alpha3 subunit | - |
3.4.25.1 | Trypanosoma brucei | Q9GU37 | alpha1 subunit | - |
3.4.25.1 | Trypanosoma brucei | Q9NDA1 | beta3 subunit | - |
3.4.25.1 | Trypanosoma brucei | Q9NDA2 | alpha4 subunit | - |
3.4.25.1 | Trypanosoma brucei | Q9NDA3 | alpha7 subunit | - |
3.4.25.1 | Trypanosoma brucei | Q9NHC5 | beta5 subunit | - |
3.4.25.1 | Trypanosoma brucei | Q9NHC6 | beta4 subunit | - |
3.4.25.1 | Trypanosoma brucei | Q9U793 | alpha2 subunit | - |
3.4.25.1 | Trypanosoma brucei | Q9U794 | beta6 subunit | - |
3.4.25.1 | Trypanosoma brucei | Q9XZG5 | alpha5 subunit | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.4.25.1 | proteolytic modification | the 20S proteasome is made up of seven alpha-subunits and seven beta-subunits. Of the seven beta-type subunits, five contain pro-sequences that are proteolytically removed during assembly, and three of them are predicted to be catalytic based on primary sequence | Trypanosoma brucei |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.25.1 | - |
Trypanosoma brucei |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.25.1 | 4-hydroxy-3-nitrophenol-Leu-Leu-Asn-vinylsulfone + H2O | - |
Trypanosoma brucei | ? | - |
? | |
3.4.25.1 | 4-hydroxy-3-nitrophenyl-Leu-Leu-Lys-vinylsulfone + H2O | - |
Trypanosoma brucei | ? | - |
? | |
3.4.25.1 | acetyl-EPFD-7-amido-4-carbamoylcoumarin + H2O | - |
Homo sapiens | ? | - |
? | |
3.4.25.1 | acetyl-EPFD-7-amido-4-carbamoylcoumarin + H2O | - |
Trypanosoma brucei | ? | - |
? | |
3.4.25.1 | acetyl-HHSL-7-amido-4-carbamoylcoumarin + H2O | - |
Homo sapiens | ? | - |
? | |
3.4.25.1 | acetyl-norleucine-Arg-norleucine-Arg-7-amido-4-carbamoylcoumarin + H2O | - |
Homo sapiens | ? | - |
? | |
3.4.25.1 | acetyl-norleucine-Arg-norleucine-Arg-7-amido-4-carbamoylcoumarin + H2O | - |
Trypanosoma brucei | ? | - |
? | |
3.4.25.1 | acetyl-YWTQ-7-amido-4-carbamoylcoumarin + H2O | - |
Homo sapiens | ? | - |
? | |
3.4.25.1 | acetyl-YWTQ-7-amido-4-carbamoylcoumarin + H2O | - |
Trypanosoma brucei | ? | - |
? | |
3.4.25.1 | Gly-Gly-Arg-7-amido-4-methylcoumarin + H2O | - |
Trypanosoma brucei | ? | - |
? | |
3.4.25.1 | additional information | overall substrate preference for hydrophobic residues at the P1 to P4 positions in a substrate. This overall stringency is relaxed in the 11 S regulatory (PA26)-20 S proteasome complex, which shows both appreciable activities for cleavage after acidic amino acids and a broadened activity for cleavage after basic amino acids. The 20S proteasome shows appreciable activity for cleavage after P1-Gln that is minimally observed in the human counterpart | Trypanosoma brucei | ? | - |
? | |
3.4.25.1 | additional information | the 20S proteasome of Trypanosoma brucei shows appreciable activity for cleavage after P1-Gln that is minimally observed in the human counterpart | Homo sapiens | ? | - |
? | |
3.4.25.1 | succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O | - |
Trypanosoma brucei | ? | - |
? | |
3.4.25.1 | Tyr-Leu-Leu-Leu-vinylsulfone + H2O | - |
Trypanosoma brucei | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.25.1 | tetradecamer | 1 * 28000, alpha1, + 1 * 24300, alpha2, + 1 * 33000, alpha3, + 1 * 27000, alpha4, + 1 * 27500, alpha5, + 1 * 29000, alpha6, + 1 * 25000, alpha7, + 1 * 24700, beta1, + 1 * 24000, beta2, + 1 * 23200, beta3, + 1 * 23800, beta4, + 1 * 22700, beta5, + 1 * 24500, beta6, + 1 * 24000, beta7, the 20S proteasome is made up of seven alpha-subunits and seven beta-subunits. Of the seven beta-type subunits, five contain pro-sequences that are proteolytically removed during assembly, and three of them are predicted to be catalytic based on primary sequence | Trypanosoma brucei |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.25.1 | 20S proteasome | - |
Homo sapiens |
3.4.25.1 | 20S proteasome | - |
Trypanosoma brucei |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.25.1 | 0.031 | 0.51 | acetyl-norleucine-Arg-norleucine-Arg-7-amido-4-carbamoylcoumarin | pH 7.8, 25°C | Trypanosoma brucei | |
3.4.25.1 | 0.27 | - |
acetyl-YWTQ-7-amido-4-carbamoylcoumarin | pH 7.8, 25°C | Homo sapiens | |
3.4.25.1 | 0.97 | - |
acetyl-EPFD-7-amido-4-carbamoylcoumarin | pH 7.8, 25°C | Homo sapiens | |
3.4.25.1 | 2.7 | - |
acetyl-EPFD-7-amido-4-carbamoylcoumarin | pH 7.8, 25°C | Trypanosoma brucei | |
3.4.25.1 | 3.3 | - |
acetyl-norleucine-Arg-norleucine-Arg-7-amido-4-carbamoylcoumarin | pH 7.8, 25°C | Homo sapiens | |
3.4.25.1 | 3.3 | - |
acetyl-YWTQ-7-amido-4-carbamoylcoumarin | pH 7.8, 25°C | Trypanosoma brucei | |
3.4.25.1 | 4.67 | - |
acetyl-YWTQ-7-amido-4-carbamoylcoumarin | pH 7.8, 25°C | Trypanosoma brucei | |
3.4.25.1 | 5.34 | - |
acetyl-norleucine-Arg-norleucine-Arg-7-amido-4-carbamoylcoumarin | pH 7.8, 25°C | Homo sapiens | |
3.4.25.1 | 8.15 | - |
acetyl-norleucine-Arg-norleucine-Arg-7-amido-4-carbamoylcoumarin | pH 7.8, 25°C | Trypanosoma brucei | |
3.4.25.1 | 36.3 | - |
acetyl-HHSL-7-amido-4-carbamoylcoumarin | pH 7.5, 25°C | Homo sapiens |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.4.25.1 | Trypanosoma brucei | pI-values of the subunits | - |
additional information |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
3.4.25.1 | 0.007 | - |
IC50: 0.04 mM, inhibition of hydrolysis of Gly-Gly-Arg-7-amido-4-methylcoumarin. IC50: 0.007 mM, inhibition of hydrolysis of succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin, most potent inhibitor for hydrolysis of succinyl-Leu-Leu-Val-Tyr-7-amido-4-meth | Trypanosoma brucei | NPLLLVS | |
3.4.25.1 | 0.033 | - |
IC50: 0.048 mM, inhibition of hydrolysis of Gly-Gly-Arg-7-amido-4-methylcoumarin. IC50: 0.033 mM, inhibition of hydrolysis of succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin | Trypanosoma brucei | YLLLVS | |
3.4.25.1 | 0.077 | - |
IC50: 0.063 mM, inhibition of hydrolysis of Gly-Gly-Arg-7-amido-4-carbamoylcoumarin. IC50: 0.077 mM, inhibition of hydrolysis of succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin | Trypanosoma brucei | NPLLNVS | |
3.4.25.1 | 0.1 | - |
IC50: 0.0015 mM, inhibition of hydrolysis of Gly-Gly-Arg-7-amido-4-methylcoumarin, most potent inhibitor for hydrolysis of Gly-Gly-Arg-7-amido-4-methylcoumarin. IC50: 0.1 mM, inhibition of hydrolysis of succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin | Trypanosoma brucei | leupeptin |