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Literature summary extracted from

  • Wang, C.C.; Bozdech, Z.; Liu, C.l.; Shipway, A.; Backes, B.J.; Harris, J.L.; Bogyo, M.
    Biochemical analysis of the 20 S proteasome of Trypanosoma brucei (2003), J. Biol. Chem., 278, 15800-15808.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.25.1 leupeptin IC50: 0.0015 mM, inhibition of hydrolysis of Gly-Gly-Arg-7-amido-4-methylcoumarin, most potent inhibitor for hydrolysis of Gly-Gly-Arg-7-amido-4-methylcoumarin. IC50: 0.1 mM, inhibition of hydrolysis of succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin Trypanosoma brucei
3.4.25.1 NPLLLVS IC50: 0.04 mM, inhibition of hydrolysis of Gly-Gly-Arg-7-amido-4-methylcoumarin. IC50: 0.007 mM, inhibition of hydrolysis of succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin, most potent inhibitor for hydrolysis of succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin Trypanosoma brucei
3.4.25.1 NPLLNVS IC50: 0.063 mM, inhibition of hydrolysis of Gly-Gly-Arg-7-amido-4-carbamoylcoumarin. IC50: 0.077 mM, inhibition of hydrolysis of succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin Trypanosoma brucei
3.4.25.1 YLLLVS IC50: 0.048 mM, inhibition of hydrolysis of Gly-Gly-Arg-7-amido-4-methylcoumarin. IC50: 0.033 mM, inhibition of hydrolysis of succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin Trypanosoma brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.25.1 0.027
-
acetyl-YWTQ-7-amido-4-carbamoylcoumarin pH 7.8, 25°C Homo sapiens
3.4.25.1 0.032
-
acetyl-EPFD-7-amido-4-carbamoylcoumarin pH 7.8, 25°C Homo sapiens
3.4.25.1 0.038
-
acetyl-YWTQ-7-amido-4-carbamoylcoumarin pH 7.8, 25°C Trypanosoma brucei
3.4.25.1 0.088
-
acetyl-EPFD-7-amido-4-carbamoylcoumarin pH 7.8, 25°C Trypanosoma brucei
3.4.25.1 0.11
-
acetyl-norleucine-Arg-norleucine-Arg-7-amido-4-carbamoylcoumarin pH 7.8, 25°C Homo sapiens
3.4.25.1 0.36
-
acetyl-HHSL-7-amido-4-carbamoylcoumarin pH 7.5, 25°C Homo sapiens
3.4.25.1 0.67
-
acetyl-norleucine-Arg-norleucine-Arg-7-amido-4-carbamoylcoumarin pH 7.8, 25°C Trypanosoma brucei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.25.1 22700
-
1 * 28000, alpha1, + 1 * 24300, alpha2, + 1 * 33000, alpha3, + 1 * 27000, alpha4, + 1 * 27500, alpha5, + 1 * 29000, alpha6, + 1 * 25000, alpha7, + 1 * 24700, beta1, + 1 * 24000, beta2, + 1 * 23200, beta3, + 1 * 23800, beta4, + 1 * 22700, beta5, + 1 * 24500, beta6, + 1 * 24000, beta7, the 20S proteasome is made up of seven alpha-subunits and seven beta-subunits. Of the seven beta-type subunits, five contain pro-sequences that are proteolytically removed during assembly, and three of them are predicted to be catalytic based on primary sequence Trypanosoma brucei
3.4.25.1 23200
-
1 * 28000, alpha1, + 1 * 24300, alpha2, + 1 * 33000, alpha3, + 1 * 27000, alpha4, + 1 * 27500, alpha5, + 1 * 29000, alpha6, + 1 * 25000, alpha7, + 1 * 24700, beta1, + 1 * 24000, beta2, + 1 * 23200, beta3, + 1 * 23800, beta4, + 1 * 22700, beta5, + 1 * 24500, beta6, + 1 * 24000, beta7, the 20S proteasome is made up of seven alpha-subunits and seven beta-subunits. Of the seven beta-type subunits, five contain pro-sequences that are proteolytically removed during assembly, and three of them are predicted to be catalytic based on primary sequence Trypanosoma brucei
3.4.25.1 23800
-
1 * 28000, alpha1, + 1 * 24300, alpha2, + 1 * 33000, alpha3, + 1 * 27000, alpha4, + 1 * 27500, alpha5, + 1 * 29000, alpha6, + 1 * 25000, alpha7, + 1 * 24700, beta1, + 1 * 24000, beta2, + 1 * 23200, beta3, + 1 * 23800, beta4, + 1 * 22700, beta5, + 1 * 24500, beta6, + 1 * 24000, beta7, the 20S proteasome is made up of seven alpha-subunits and seven beta-subunits. Of the seven beta-type subunits, five contain pro-sequences that are proteolytically removed during assembly, and three of them are predicted to be catalytic based on primary sequence Trypanosoma brucei
3.4.25.1 24000
-
1 * 28000, alpha1, + 1 * 24300, alpha2, + 1 * 33000, alpha3, + 1 * 27000, alpha4, + 1 * 27500, alpha5, + 1 * 29000, alpha6, + 1 * 25000, alpha7, + 1 * 24700, beta1, + 1 * 24000, beta2, + 1 * 23200, beta3, + 1 * 23800, beta4, + 1 * 22700, beta5, + 1 * 24500, beta6, + 1 * 24000, beta7, the 20S proteasome is made up of seven alpha-subunits and seven beta-subunits. Of the seven beta-type subunits, five contain pro-sequences that are proteolytically removed during assembly, and three of them are predicted to be catalytic based on primary sequence Trypanosoma brucei
3.4.25.1 24300
-
1 * 28000, alpha1, + 1 * 24300, alpha2, + 1 * 33000, alpha3, + 1 * 27000, alpha4, + 1 * 27500, alpha5, + 1 * 29000, alpha6, + 1 * 25000, alpha7, + 1 * 24700, beta1, + 1 * 24000, beta2, + 1 * 23200, beta3, + 1 * 23800, beta4, + 1 * 22700, beta5, + 1 * 24500, beta6, + 1 * 24000, beta7, the 20S proteasome is made up of seven alpha-subunits and seven beta-subunits. Of the seven beta-type subunits, five contain pro-sequences that are proteolytically removed during assembly, and three of them are predicted to be catalytic based on primary sequence Trypanosoma brucei
3.4.25.1 24500
-
1 * 28000, alpha1, + 1 * 24300, alpha2, + 1 * 33000, alpha3, + 1 * 27000, alpha4, + 1 * 27500, alpha5, + 1 * 29000, alpha6, + 1 * 25000, alpha7, + 1 * 24700, beta1, + 1 * 24000, beta2, + 1 * 23200, beta3, + 1 * 23800, beta4, + 1 * 22700, beta5, + 1 * 24500, beta6, + 1 * 24000, beta7, the 20S proteasome is made up of seven alpha-subunits and seven beta-subunits. Of the seven beta-type subunits, five contain pro-sequences that are proteolytically removed during assembly, and three of them are predicted to be catalytic based on primary sequence Trypanosoma brucei
3.4.25.1 24700
-
1 * 28000, alpha1, + 1 * 24300, alpha2, + 1 * 33000, alpha3, + 1 * 27000, alpha4, + 1 * 27500, alpha5, + 1 * 29000, alpha6, + 1 * 25000, alpha7, + 1 * 24700, beta1, + 1 * 24000, beta2, + 1 * 23200, beta3, + 1 * 23800, beta4, + 1 * 22700, beta5, + 1 * 24500, beta6, + 1 * 24000, beta7, the 20S proteasome is made up of seven alpha-subunits and seven beta-subunits. Of the seven beta-type subunits, five contain pro-sequences that are proteolytically removed during assembly, and three of them are predicted to be catalytic based on primary sequence Trypanosoma brucei
3.4.25.1 25000
-
1 * 28000, alpha1, + 1 * 24300, alpha2, + 1 * 33000, alpha3, + 1 * 27000, alpha4, + 1 * 27500, alpha5, + 1 * 29000, alpha6, + 1 * 25000, alpha7, + 1 * 24700, beta1, + 1 * 24000, beta2, + 1 * 23200, beta3, + 1 * 23800, beta4, + 1 * 22700, beta5, + 1 * 24500, beta6, + 1 * 24000, beta7, the 20S proteasome is made up of seven alpha-subunits and seven beta-subunits. Of the seven beta-type subunits, five contain pro-sequences that are proteolytically removed during assembly, and three of them are predicted to be catalytic based on primary sequence Trypanosoma brucei
3.4.25.1 27000
-
1 * 28000, alpha1, + 1 * 24300, alpha2, + 1 * 33000, alpha3, + 1 * 27000, alpha4, + 1 * 27500, alpha5, + 1 * 29000, alpha6, + 1 * 25000, alpha7, + 1 * 24700, beta1, + 1 * 24000, beta2, + 1 * 23200, beta3, + 1 * 23800, beta4, + 1 * 22700, beta5, + 1 * 24500, beta6, + 1 * 24000, beta7, the 20S proteasome is made up of seven alpha-subunits and seven beta-subunits. Of the seven beta-type subunits, five contain pro-sequences that are proteolytically removed during assembly, and three of them are predicted to be catalytic based on primary sequence Trypanosoma brucei
3.4.25.1 27500
-
1 * 28000, alpha1, + 1 * 24300, alpha2, + 1 * 33000, alpha3, + 1 * 27000, alpha4, + 1 * 27500, alpha5, + 1 * 29000, alpha6, + 1 * 25000, alpha7, + 1 * 24700, beta1, + 1 * 24000, beta2, + 1 * 23200, beta3, + 1 * 23800, beta4, + 1 * 22700, beta5, + 1 * 24500, beta6, + 1 * 24000, beta7, the 20S proteasome is made up of seven alpha-subunits and seven beta-subunits. Of the seven beta-type subunits, five contain pro-sequences that are proteolytically removed during assembly, and three of them are predicted to be catalytic based on primary sequence Trypanosoma brucei
3.4.25.1 28000
-
1 * 28000, alpha1, + 1 * 24300, alpha2, + 1 * 33000, alpha3, + 1 * 27000, alpha4, + 1 * 27500, alpha5, + 1 * 29000, alpha6, + 1 * 25000, alpha7, + 1 * 24700, beta1, + 1 * 24000, beta2, + 1 * 23200, beta3, + 1 * 23800, beta4, + 1 * 22700, beta5, + 1 * 24500, beta6, + 1 * 24000, beta7, the 20S proteasome is made up of seven alpha-subunits and seven beta-subunits. Of the seven beta-type subunits, five contain pro-sequences that are proteolytically removed during assembly, and three of them are predicted to be catalytic based on primary sequence Trypanosoma brucei
3.4.25.1 29000
-
1 * 28000, alpha1, + 1 * 24300, alpha2, + 1 * 33000, alpha3, + 1 * 27000, alpha4, + 1 * 27500, alpha5, + 1 * 29000, alpha6, + 1 * 25000, alpha7, + 1 * 24700, beta1, + 1 * 24000, beta2, + 1 * 23200, beta3, + 1 * 23800, beta4, + 1 * 22700, beta5, + 1 * 24500, beta6, + 1 * 24000, beta7, the 20S proteasome is made up of seven alpha-subunits and seven beta-subunits. Of the seven beta-type subunits, five contain pro-sequences that are proteolytically removed during assembly, and three of them are predicted to be catalytic based on primary sequence Trypanosoma brucei
3.4.25.1 33000
-
1 * 28000, alpha1, + 1 * 24300, alpha2, + 1 * 33000, alpha3, + 1 * 27000, alpha4, + 1 * 27500, alpha5, + 1 * 29000, alpha6, + 1 * 25000, alpha7, + 1 * 24700, beta1, + 1 * 24000, beta2, + 1 * 23200, beta3, + 1 * 23800, beta4, + 1 * 22700, beta5, + 1 * 24500, beta6, + 1 * 24000, beta7, the 20S proteasome is made up of seven alpha-subunits and seven beta-subunits. Of the seven beta-type subunits, five contain pro-sequences that are proteolytically removed during assembly, and three of them are predicted to be catalytic based on primary sequence Trypanosoma brucei

Organism

EC Number Organism UniProt Comment Textmining
3.4.25.1 Homo sapiens
-
-
-
3.4.25.1 Trypanosoma brucei O96780 beta2 subunit
-
3.4.25.1 Trypanosoma brucei O96787 beta1 subunit
-
3.4.25.1 Trypanosoma brucei O96788 alpha6 subunit
-
3.4.25.1 Trypanosoma brucei Q9BMX8 beta7 subunit
-
3.4.25.1 Trypanosoma brucei Q9GU36 alpha3 subunit
-
3.4.25.1 Trypanosoma brucei Q9GU37 alpha1 subunit
-
3.4.25.1 Trypanosoma brucei Q9NDA1 beta3 subunit
-
3.4.25.1 Trypanosoma brucei Q9NDA2 alpha4 subunit
-
3.4.25.1 Trypanosoma brucei Q9NDA3 alpha7 subunit
-
3.4.25.1 Trypanosoma brucei Q9NHC5 beta5 subunit
-
3.4.25.1 Trypanosoma brucei Q9NHC6 beta4 subunit
-
3.4.25.1 Trypanosoma brucei Q9U793 alpha2 subunit
-
3.4.25.1 Trypanosoma brucei Q9U794 beta6 subunit
-
3.4.25.1 Trypanosoma brucei Q9XZG5 alpha5 subunit
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.25.1 proteolytic modification the 20S proteasome is made up of seven alpha-subunits and seven beta-subunits. Of the seven beta-type subunits, five contain pro-sequences that are proteolytically removed during assembly, and three of them are predicted to be catalytic based on primary sequence Trypanosoma brucei

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.25.1
-
Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.25.1 4-hydroxy-3-nitrophenol-Leu-Leu-Asn-vinylsulfone + H2O
-
Trypanosoma brucei ?
-
?
3.4.25.1 4-hydroxy-3-nitrophenyl-Leu-Leu-Lys-vinylsulfone + H2O
-
Trypanosoma brucei ?
-
?
3.4.25.1 acetyl-EPFD-7-amido-4-carbamoylcoumarin + H2O
-
Homo sapiens ?
-
?
3.4.25.1 acetyl-EPFD-7-amido-4-carbamoylcoumarin + H2O
-
Trypanosoma brucei ?
-
?
3.4.25.1 acetyl-HHSL-7-amido-4-carbamoylcoumarin + H2O
-
Homo sapiens ?
-
?
3.4.25.1 acetyl-norleucine-Arg-norleucine-Arg-7-amido-4-carbamoylcoumarin + H2O
-
Homo sapiens ?
-
?
3.4.25.1 acetyl-norleucine-Arg-norleucine-Arg-7-amido-4-carbamoylcoumarin + H2O
-
Trypanosoma brucei ?
-
?
3.4.25.1 acetyl-YWTQ-7-amido-4-carbamoylcoumarin + H2O
-
Homo sapiens ?
-
?
3.4.25.1 acetyl-YWTQ-7-amido-4-carbamoylcoumarin + H2O
-
Trypanosoma brucei ?
-
?
3.4.25.1 Gly-Gly-Arg-7-amido-4-methylcoumarin + H2O
-
Trypanosoma brucei ?
-
?
3.4.25.1 additional information overall substrate preference for hydrophobic residues at the P1 to P4 positions in a substrate. This overall stringency is relaxed in the 11 S regulatory (PA26)-20 S proteasome complex, which shows both appreciable activities for cleavage after acidic amino acids and a broadened activity for cleavage after basic amino acids. The 20S proteasome shows appreciable activity for cleavage after P1-Gln that is minimally observed in the human counterpart Trypanosoma brucei ?
-
?
3.4.25.1 additional information the 20S proteasome of Trypanosoma brucei shows appreciable activity for cleavage after P1-Gln that is minimally observed in the human counterpart Homo sapiens ?
-
?
3.4.25.1 succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O
-
Trypanosoma brucei ?
-
?
3.4.25.1 Tyr-Leu-Leu-Leu-vinylsulfone + H2O
-
Trypanosoma brucei ?
-
?

Subunits

EC Number Subunits Comment Organism
3.4.25.1 tetradecamer 1 * 28000, alpha1, + 1 * 24300, alpha2, + 1 * 33000, alpha3, + 1 * 27000, alpha4, + 1 * 27500, alpha5, + 1 * 29000, alpha6, + 1 * 25000, alpha7, + 1 * 24700, beta1, + 1 * 24000, beta2, + 1 * 23200, beta3, + 1 * 23800, beta4, + 1 * 22700, beta5, + 1 * 24500, beta6, + 1 * 24000, beta7, the 20S proteasome is made up of seven alpha-subunits and seven beta-subunits. Of the seven beta-type subunits, five contain pro-sequences that are proteolytically removed during assembly, and three of them are predicted to be catalytic based on primary sequence Trypanosoma brucei

Synonyms

EC Number Synonyms Comment Organism
3.4.25.1 20S proteasome
-
Homo sapiens
3.4.25.1 20S proteasome
-
Trypanosoma brucei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.25.1 0.031 0.51 acetyl-norleucine-Arg-norleucine-Arg-7-amido-4-carbamoylcoumarin pH 7.8, 25°C Trypanosoma brucei
3.4.25.1 0.27
-
acetyl-YWTQ-7-amido-4-carbamoylcoumarin pH 7.8, 25°C Homo sapiens
3.4.25.1 0.97
-
acetyl-EPFD-7-amido-4-carbamoylcoumarin pH 7.8, 25°C Homo sapiens
3.4.25.1 2.7
-
acetyl-EPFD-7-amido-4-carbamoylcoumarin pH 7.8, 25°C Trypanosoma brucei
3.4.25.1 3.3
-
acetyl-norleucine-Arg-norleucine-Arg-7-amido-4-carbamoylcoumarin pH 7.8, 25°C Homo sapiens
3.4.25.1 3.3
-
acetyl-YWTQ-7-amido-4-carbamoylcoumarin pH 7.8, 25°C Trypanosoma brucei
3.4.25.1 4.67
-
acetyl-YWTQ-7-amido-4-carbamoylcoumarin pH 7.8, 25°C Trypanosoma brucei
3.4.25.1 5.34
-
acetyl-norleucine-Arg-norleucine-Arg-7-amido-4-carbamoylcoumarin pH 7.8, 25°C Homo sapiens
3.4.25.1 8.15
-
acetyl-norleucine-Arg-norleucine-Arg-7-amido-4-carbamoylcoumarin pH 7.8, 25°C Trypanosoma brucei
3.4.25.1 36.3
-
acetyl-HHSL-7-amido-4-carbamoylcoumarin pH 7.5, 25°C Homo sapiens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.25.1 Trypanosoma brucei pI-values of the subunits
-
additional information

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.4.25.1 0.007
-
IC50: 0.04 mM, inhibition of hydrolysis of Gly-Gly-Arg-7-amido-4-methylcoumarin. IC50: 0.007 mM, inhibition of hydrolysis of succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin, most potent inhibitor for hydrolysis of succinyl-Leu-Leu-Val-Tyr-7-amido-4-meth Trypanosoma brucei NPLLLVS
3.4.25.1 0.033
-
IC50: 0.048 mM, inhibition of hydrolysis of Gly-Gly-Arg-7-amido-4-methylcoumarin. IC50: 0.033 mM, inhibition of hydrolysis of succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin Trypanosoma brucei YLLLVS
3.4.25.1 0.077
-
IC50: 0.063 mM, inhibition of hydrolysis of Gly-Gly-Arg-7-amido-4-carbamoylcoumarin. IC50: 0.077 mM, inhibition of hydrolysis of succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin Trypanosoma brucei NPLLNVS
3.4.25.1 0.1
-
IC50: 0.0015 mM, inhibition of hydrolysis of Gly-Gly-Arg-7-amido-4-methylcoumarin, most potent inhibitor for hydrolysis of Gly-Gly-Arg-7-amido-4-methylcoumarin. IC50: 0.1 mM, inhibition of hydrolysis of succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin Trypanosoma brucei leupeptin