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Literature summary extracted from

  • Chamond, N.; Gregoire, C.; Coatnoan, N.; Rougeot, C.; Freitas-Junior, L.H.; da Silveira, J.F.; Degrave, W.M.; Minoprio, P.
    Biochemical characterization of proline racemases from the human protozoan parasite Trypanosoma cruzi and definition of putative protein signatures (2003), J. Biol. Chem., 278, 15484-15494.
    View publication on PubMed

Application

EC Number Application Comment Organism
5.1.1.4 medicine potential of the enzyme as a critical target for drug development against Chagas‘ disease. The enzyme is absent in mammalian host, suggesting that inhibition of proline racemase may have therapeutic potential Trypanosoma cruzi
5.1.1.4 medicine potential of the enzyme as a critical target for drug development against Chaga‘s disease. The enzyme is absent in mammalian host, suggesting that inhibition of proline racemase may have therapeutic potential Trypanosoma cruzi

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.1.4 expression in Escherichia coli Trypanosoma cruzi

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.1.4 pyrrole-2-carboxylic acid
-
 Trypanosoma cruzi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.1.4 29
-
 L-proline pH 6.0, 37°C Trypanosoma cruzi
5.1.1.4 75
-
 L-proline pH 6.0, 37°C Trypanosoma cruzi

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.1.1.4 extracellular membrane-bound and secreted forms of enzyme are present upon differentiation of the parasite into non-dividing infective forms Trypanosoma cruzi
-
-
5.1.1.4 intracellular in replicative non-infective forms of Trypanosoma cruzi Trypanosoma cruzi 5622
-
5.1.1.4 membrane membrane-bound and secreted forms of enzyme are present upon differentiation of the parasite into non-dividing infective forms Trypanosoma cruzi 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.1.4 40100
-
 2 * 40100, TcPRACB, SDS-PAGE Trypanosoma cruzi
5.1.1.4 45800
-
 2 * 45800, TcPRACA, SDS-PAGE Trypanosoma cruzi
5.1.1.4 80000
-
 TCPRACA, gel filtration, non-denaturing PAGE Trypanosoma cruzi

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.4 Trypanosoma cruzi Q4DA80
-
-
5.1.1.4 Trypanosoma cruzi Q868H8
-
-

Storage Stability

EC Number Storage Stability Organism
5.1.1.4 -20°C, 50% glycerol or diluted in sodium acetate buffer, pH 6.0, activity is impaired Trypanosoma cruzi
5.1.1.4 -20°C, 50% glycerol or diluted in sodium acetate buffer, pH 6.0, stable Trypanosoma cruzi
5.1.1.4 -20°C, as ammonium sulfate precipitate, stable Trypanosoma cruzi
5.1.1.4 23°C, 0.5 M imidazole buffer, pH 8.0, 10 days, TcPRACA loses 84% of its activity Trypanosoma cruzi
5.1.1.4 23°C, 0.5 M imidazole buffer, pH 8.0, 10 days, TcPRACB is stable, TcPRACA loses 84% of its activity Trypanosoma cruzi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.4 L-proline
-
 Trypanosoma cruzi D-proline
-
 ?
5.1.1.4 additional information TcPRACB: no reaction with L-hydroxyproline Trypanosoma cruzi ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.1.1.4 dimer 2 * 40100, TcPRACB, SDS-PAGE Trypanosoma cruzi
5.1.1.4 dimer 2 * 45800, TcPRACA, SDS-PAGE Trypanosoma cruzi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.1.4 37
-
-
 Trypanosoma cruzi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.1.1.4 80
-
 5 min, activity is abolished Trypanosoma cruzi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.1.4 4.5 8.5
-
 Trypanosoma cruzi
5.1.1.4 5.5 7
-
 Trypanosoma cruzi

Cofactor

EC Number Cofactor Comment Organism Structure
5.1.1.4 additional information TcPRACB is a cofactor-independent racemase Trypanosoma cruzi

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.1.1.4 0.0036
-
 pyrrole-2-carboxylic acid pH 6.0, 37°C, TcPRACB Trypanosoma cruzi
5.1.1.4 0.0057
-
 pyrrole-2-carboxylic acid pH 6.0, 37°C, TcPRACA Trypanosoma cruzi