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Literature summary extracted from

  • Valkova, N.; Lepine, F.; Labrie, L.; Dupont, M.; Beaudet, R.
    Purification and characterization of PrbA, a new esterase from Enterobacter cloacae hydrolyzing the esters of 4-hydroxybenzoic acid (parabens) (2003), J. Biol. Chem., 278, 12779-12785.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 1-chloro-3-tosylamido-4-phenyl-2-butanone
-
 Enterobacter cloacae
3.1.1.1 1-chloro-3-tosylamido-7-amino-2-heptanone
-
 Enterobacter cloacae
3.1.1.1 diethyl dicarbonate irreversible inhibition Enterobacter cloacae
3.1.1.1 diisopropyl fluorophosphate irreversible inhibition Enterobacter cloacae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.1 0.45
-
 butyl paraben pH 6.8, 30°C Enterobacter cloacae
3.1.1.1 0.6
-
 propyl paraben pH 6.8, 30°C Enterobacter cloacae
3.1.1.1 0.88
-
 methyl paraben pH 6.8, 30°C Enterobacter cloacae
3.1.1.1 3.7
-
 4-nitrophenyl acetate pH 6.8, 30°C Enterobacter cloacae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 54620
-
 mass spectrometry Enterobacter cloacae

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Enterobacter cloacae
-
 gene name prbA
-
3.1.1.1 Enterobacter cloacae EM
-
 gene name prbA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1 60fold Enterobacter cloacae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.1 0.00023
-
 purified enzyme, substrate 4-nitrophenyl acetate Enterobacter cloacae
3.1.1.1 0.0009
-
 purified enzyme, substrate butyl paraben Enterobacter cloacae
3.1.1.1 0.0019
-
 purified enzyme, substrate propyl paraben Enterobacter cloacae
3.1.1.1 0.0025
-
 purified enzyme, substrate methyl paraben Enterobacter cloacae
3.1.1.1 0.0027
-
 purified enzyme, substrate ethyl paraben Enterobacter cloacae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 4-nitrophenyl acetate + H2O
-
 Enterobacter cloacae 4-nitrophenol + acetate
-
 ?
3.1.1.1 4-nitrophenyl acetate + H2O
-
 Enterobacter cloacae EM 4-nitrophenol + acetate
-
 ?
3.1.1.1 butyl paraben + H2O
-
 Enterobacter cloacae butanol + 4-hydroxybenzoate
-
 ?
3.1.1.1 ethyl 3,4-dihydroxybenzoate + H2O low activity Enterobacter cloacae ethanol + 3,4-dihydroxybenzoate
-
 ?
3.1.1.1 ethyl paraben + H2O best substrate Enterobacter cloacae ethanol + 4-hydroxybenzoate
-
 ?
3.1.1.1 ethyl paraben + H2O best substrate Enterobacter cloacae EM ethanol + 4-hydroxybenzoate
-
 ?
3.1.1.1 methyl 2-aminobenzoate + H2O
-
 Enterobacter cloacae methanol + 2-aminobenzoate
-
 ?
3.1.1.1 methyl 2-hydroxybenzoate + H2O very low activity Enterobacter cloacae methanol + 2-hydroxybenzoate
-
 ?
3.1.1.1 methyl 3,5-dihydroxybenzoate + H2O low activity Enterobacter cloacae methanol + 3-hydroxybenzoate
-
 ?
3.1.1.1 methyl 3-hydroxybenzoate + H2O
-
 Enterobacter cloacae methanol + 3-hydroxybenzoate
-
 ?
3.1.1.1 methyl 4-aminobenzoate + H2O
-
 Enterobacter cloacae methanol + 4-aminobenzoate
-
 ?
3.1.1.1 methyl benzoate + H2O low activity Enterobacter cloacae methanol + benzoate
-
 ?
3.1.1.1 methyl paraben + H2O
-
 Enterobacter cloacae methanol + 4-hydroxybenzoate
-
 ?
3.1.1.1 methyl paraben + H2O
-
 Enterobacter cloacae EM methanol + 4-hydroxybenzoate
-
 ?
3.1.1.1 methyl vanillate + H2O
-
 Enterobacter cloacae methanol + vanillate
-
 ?
3.1.1.1 additional information the enzyme also performs transesterification reactions in competition to the hydrolysis, overview Enterobacter cloacae ?
-
 ?
3.1.1.1 additional information the enzyme also performs transesterification reactions in competition to the hydrolysis, overview Enterobacter cloacae EM ?
-
 ?
3.1.1.1 propyl paraben + H2O
-
 Enterobacter cloacae propanol + 4-hydroxybenzoate
-
 ?
3.1.1.1 propyl paraben + H2O
-
 Enterobacter cloacae EM propanol + 4-hydroxybenzoate
-
 ?
3.1.1.1 trans-methyl cinnamate + H2O very low activity Enterobacter cloacae methanol + cinnamate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.1 PrbA
-
 Enterobacter cloacae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 31
-
-
 Enterobacter cloacae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.1 15 50 more than 50% of maximal activity at 15°C and 50°C Enterobacter cloacae
3.1.1.1 29 35 90% of maximal activity at 29°C and 35°C Enterobacter cloacae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 7
-
-
 Enterobacter cloacae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.1 4 8 70-80% of maximal activity at pH 4.0 and pH 8.0 Enterobacter cloacae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.1.1 0.2
-
 1-chloro-3-tosylamido-7-amino-2-heptanone pH 6.8, 30°C Enterobacter cloacae
3.1.1.1 0.29
-
 1-chloro-3-tosylamido-4-phenyl-2-butanone pH 6.8, 30°C Enterobacter cloacae