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Literature summary extracted from
- Functional characterization of DELTA3,DELTA2-enoyl-CoA isomerases from rat liver (2002), J. Biol. Chem., 277, 9127-9132.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.3.8 | 0.0044 | - |
2-trans,5-cis-octadienoyl-CoA | pH 8.0, 25°C, MFE1 | Rattus norvegicus |  |
| 5.3.3.8 | 0.0096 | - |
2-trans,5-cis-tetradecadienoyl-CoA | pH 8.0, 25°C, MFE1 | Rattus norvegicus | |
| 5.3.3.8 | 0.02 | - |
2-trans,5-cis-tetradecadienoyl-CoA | pH 8.0, 25°C, MECI | Rattus norvegicus | |
| 5.3.3.8 | 0.021 | - |
3-cis-tetradecenoyl-CoA | pH 8.0, 25°C, ECI | Rattus norvegicus | |
| 5.3.3.8 | 0.028 | - |
3-trans-octenoyl-CoA | pH 8.0, 25°C, MFE1 | Rattus norvegicus | |
| 5.3.3.8 | 0.029 | - |
2-trans,5-cis-tetradecadienoyl-CoA | pH 8.0, 25°C, ECI | Rattus norvegicus | |
| 5.3.3.8 | 0.029 | - |
3-trans-tetradecenoyl-CoA | pH 8.0, 25°C, ECI | Rattus norvegicus | |
| 5.3.3.8 | 0.031 | - |
3-cis-hexenoyl-CoA | pH 8.0, 25°C, MFE1 | Rattus norvegicus | |
| 5.3.3.8 | 0.032 | - |
3-trans-tetradecenoyl-CoA | pH 8.0, 25°C, MFE1 | Rattus norvegicus | |
| 5.3.3.8 | 0.032 | - |
3-cis-octenoyl-CoA | pH 8.0, 25°C, MFE1 | Rattus norvegicus | |
| 5.3.3.8 | 0.038 | - |
3-trans-hexenoyl-CoA | pH 8.0, 25°C, MFE1 | Rattus norvegicus | |
| 5.3.3.8 | 0.045 | - |
3-cis-tetradecenoyl-CoA | pH 8.0, 25°C, MFE1 | Rattus norvegicus | |
| 5.3.3.8 | 0.048 | - |
3-trans-tetradecenoyl-CoA | pH 8.0, 25°C, MECI | Rattus norvegicus | |
| 5.3.3.8 | 0.057 | - |
3-cis-tetradecenoyl-CoA | pH 8.0, 25°C, MECI | Rattus norvegicus | |
| 5.3.3.8 | 0.084 | - |
2-trans,5-cis-octadienoyl-CoA | pH 8.0, 25°C, MECI | Rattus norvegicus | |
| 5.3.3.8 | 0.1 | - |
3-cis-octenoyl-CoA | pH 8.0, 25°C, ECI | Rattus norvegicus | |
| 5.3.3.8 | 0.12 | - |
3-trans-octenoyl-CoA | pH 8.0, 25°C, ECI | Rattus norvegicus | |
| 5.3.3.8 | 0.15 | - |
3-cis-octenoyl-CoA | pH 8.0, 25°C, MECI | Rattus norvegicus | |
| 5.3.3.8 | 0.17 | - |
2-trans,5-cis-octadienoyl-CoA | pH 8.0, 25°C, ECI | Rattus norvegicus | |
| 5.3.3.8 | 0.19 | - |
3-trans-octenoyl-CoA | pH 8.0, 25°C, MECI | Rattus norvegicus | |
| 5.3.3.8 | 0.24 | - |
3-cis-hexenoyl-CoA | pH 8.0, 25°C, MECI | Rattus norvegicus | |
| 5.3.3.8 | 0.47 | - |
3-trans-hexenoyl-CoA | pH 8.0, 25°C, MECI | Rattus norvegicus | |
| 5.3.3.8 | 1.2 | - |
3-cis-hexenoyl-CoA | pH 8.0, 25°C, ECI | Rattus norvegicus | |
| 5.3.3.8 | 1.6 | - |
3-trans-hexenoyl-CoA | pH 8.0, 25°C, ECI | Rattus norvegicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 5.3.3.8 | mitochondrion | ECI and MECI | Rattus norvegicus | 5739 | - |
| 5.3.3.8 | peroxisome | ECI and MFE1 | Rattus norvegicus | 5777 | - |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.3.8 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.3.3.8 | ECI, MECI and MFE1 | Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.3.8 | 2-trans,5-cis-octadienoyl-CoA | - |
Rattus norvegicus | 3-cis,5-cis-octadienoyl-CoA | - |
? | |
| 5.3.3.8 | 2-trans,5-cis-tetradecadienoyl-CoA | - |
Rattus norvegicus | 3-cis,5-cis-tetradecadienoyl-CoA | - |
? | |
| 5.3.3.8 | 3-cis-hexenoyl-CoA | - |
Rattus norvegicus | 2-trans-hexenoyl-CoA | - |
? | |
| 5.3.3.8 | 3-cis-octenoyl-CoA | - |
Rattus norvegicus | 2-trans-octenoyl-CoA | - |
? | |
| 5.3.3.8 | 3-cis-Tetradecenoyl-CoA | - |
Rattus norvegicus | 2-trans-Tetradecenoyl-CoA | - |
? | |
| 5.3.3.8 | 3-trans-Hexenoyl-CoA | - |
Rattus norvegicus | 2-trans-Hexenoyl-CoA | - |
? | |
| 5.3.3.8 | 3-trans-octenoyl-CoA | - |
Rattus norvegicus | 2-trans-octenoyl-CoA | - |
? | |
| 5.3.3.8 | 3-trans-tetradecenoyl-CoA | - |
Rattus norvegicus | 2-trans-tetradecenoyl-CoA | - |
? | |
| 5.3.3.8 | additional information | MECI is most active in catalyzing the 3-cis/2-trans isomerization, ECI has a preference for the 3-trans/2-trans isomerization, and MFE1 is the optimal isomerase for the 2,5-/3,5-isomerization. 3-cis/2-trans and 2,5/3,5 isomerization in mitochondria are catalyzed overwhelmingly by MECI, whereas ECI contributes significantly to the 3-trans/2-trans isomerization. In peroxisomes, ECI is predicted to be the dominant enzyme for the 3-cis/2-trans and 3-trans/2-trans isomerization of long chain intermediates | Rattus norvegicus | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.3.3.8 | ECI | monofunctional enoyl CoA isomerase | Rattus norvegicus |
| 5.3.3.8 | MECI | mitochondrial enoyl-CoA isomerase, present only in the mitochondria | Rattus norvegicus |
| 5.3.3.8 | MFE1 | multifunctional enzyme 1 | Rattus norvegicus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.3.8 | 1.4 | - |
3-trans-tetradecenoyl-CoA | pH 8.0, 25°C, MFE1 | Rattus norvegicus | |
| 5.3.3.8 | 1.6 | - |
3-cis-hexenoyl-CoA | pH 8.0, 25°C, MFE1 | Rattus norvegicus | |
| 5.3.3.8 | 1.8 | - |
3-trans-hexenoyl-CoA | pH 8.0, 25°C, MFE1 | Rattus norvegicus | |
| 5.3.3.8 | 2 | - |
3-trans-octenoyl-CoA | pH 8.0, 25°C, MFE1 | Rattus norvegicus | |
| 5.3.3.8 | 2.4 | - |
3-cis-octenoyl-CoA | pH 8.0, 25°C, MFE1 | Rattus norvegicus | |
| 5.3.3.8 | 2.7 | - |
3-cis-tetradecenoyl-CoA | pH 8.0, 25°C, MFE1 | Rattus norvegicus | |
| 5.3.3.8 | 8.3 | - |
2-trans,5-cis-tetradecadienoyl-CoA | pH 8.0, 25°C, MECI | Rattus norvegicus | |
| 5.3.3.8 | 11 | - |
2-trans,5-cis-octadienoyl-CoA | pH 8.0, 25°C, MFE1 | Rattus norvegicus | |
| 5.3.3.8 | 12 | - |
2-trans,5-cis-tetradecadienoyl-CoA | pH 8.0, 25°C, MFE1 | Rattus norvegicus | |
| 5.3.3.8 | 15 | - |
2-trans,5-cis-tetradecadienoyl-CoA | pH 8.0, 25°C, ECI | Rattus norvegicus | |
| 5.3.3.8 | 20 | - |
3-trans-tetradecenoyl-CoA | pH 8.0, 25°C, MECI | Rattus norvegicus | |
| 5.3.3.8 | 25 | - |
3-trans-octenoyl-CoA | pH 8.0, 25°C, MECI | Rattus norvegicus | |
| 5.3.3.8 | 26 | - |
2-trans,5-cis-octadienoyl-CoA | pH 8.0, 25°C, ECI | Rattus norvegicus | |
| 5.3.3.8 | 45 | - |
2-trans,5-cis-octadienoyl-CoA | pH 8.0, 25°C, MECI | Rattus norvegicus | |
| 5.3.3.8 | 47 | - |
3-cis-octenoyl-CoA | pH 8.0, 25°C, ECI | Rattus norvegicus | |
| 5.3.3.8 | 58 | - |
3-cis-hexenoyl-CoA | pH 8.0, 25°C, ECI | Rattus norvegicus | |
| 5.3.3.8 | 98 | - |
3-cis-tetradecenoyl-CoA | pH 8.0, 25°C, MECI | Rattus norvegicus | |
| 5.3.3.8 | 100 | - |
3-trans-hexenoyl-CoA | pH 8.0, 25°C, ECI | Rattus norvegicus | |
| 5.3.3.8 | 180 | - |
3-cis-octenoyl-CoA | pH 8.0, 25°C, MECI | Rattus norvegicus | |
| 5.3.3.8 | 200 | - |
3-cis-hexenoyl-CoA | pH 8.0, 25°C, MECI | Rattus norvegicus | |
| 5.3.3.8 | 210 | - |
3-cis-tetradecenoyl-CoA | pH 8.0, 25°C, ECI | Rattus norvegicus | |
| 5.3.3.8 | 210 | - |
3-trans-octenoyl-CoA | pH 8.0, 25°C, ECI | Rattus norvegicus | |
| 5.3.3.8 | 270 | - |
3-trans-hexenoyl-CoA | pH 8.0, 25°C, MECI | Rattus norvegicus | |
| 5.3.3.8 | 540 | - |
3-trans-tetradecenoyl-CoA | pH 8.0, 25°C, ECI | Rattus norvegicus | |
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