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Literature summary extracted from

  • Soong, C.L.; Ogawa, J.; Sakuradani, E.; Shimizu, S.
    Barbiturase, a novel zinc-containing amidohydrolase involved in oxidative pyrimidine metabolism (2002), J. Biol. Chem., 277, 7051-7058.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.1
-
 Rhodococcus erythropolis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.1 2,2'-dipyridyl 2 mM, 30% inhibition Rhodococcus erythropolis
3.5.2.1 5,5'-dithio-bis-(2-nitrobenzoic acid) 2 mM, 50% inhibition Rhodococcus erythropolis
3.5.2.1 8-hydroxyquinoline 2 mM, 60% inhibition Rhodococcus erythropolis
3.5.2.1 alloxan competitive Rhodococcus erythropolis
3.5.2.1 Cd2+ 2 mM, 60% inhibition Rhodococcus erythropolis
3.5.2.1 Co2+ 2 mM, 50% inhibition Rhodococcus erythropolis
3.5.2.1 Cu2+ 2 mM, complete inhibition Rhodococcus erythropolis
3.5.2.1 cyanuric acid competitive Rhodococcus erythropolis
3.5.2.1 dihydro-L-orotate competitive Rhodococcus erythropolis
3.5.2.1 diisopropyl phosphofluoridate 2 mM, 90% inhibition Rhodococcus erythropolis
3.5.2.1 EDTA 2 mM, 30% inhibition Rhodococcus erythropolis
3.5.2.1 Hg2+ 2 mM, complete inhibition Rhodococcus erythropolis
3.5.2.1 N-bromosuccinimide 2 mM, 60% inhibition Rhodococcus erythropolis
3.5.2.1 N-ethylmaleimide 2 mM, complete inhibition Rhodococcus erythropolis
3.5.2.1 Ni2+ 2 mM, 80% inhibition Rhodococcus erythropolis
3.5.2.1 o-phenanthroline 2 mM, 80% inhibition Rhodococcus erythropolis
3.5.2.1 p-chloromercuribenzoate 2 mM, complete inhibition Rhodococcus erythropolis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.1 1
-
 Barbiturate pH 8.0, 30°C Rhodococcus erythropolis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.1 Zn2+ 4.4 mol per mol of enzyme Rhodococcus erythropolis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.1 45000
-
 4 * 45000, SDS-PAGE Rhodococcus erythropolis
3.5.2.1 172000
-
 gel filtration Rhodococcus erythropolis

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.95 Rhodococcus erythropolis
-
 JCM 3132
-
3.5.1.95 Rhodococcus erythropolis JCM 3132
-
 JCM 3132
-
3.5.2.1 Rhodococcus erythropolis Q8RSQ2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.1
-
 Rhodococcus erythropolis

Storage Stability

EC Number Storage Stability Organism
3.5.2.1 4°C, 10% (v/v) ethylene glykol, stable up to 3 months Rhodococcus erythropolis
3.5.2.1 4°C, cell-free extract, loss of 70% of activity during 2 weeks Rhodococcus erythropolis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.1 barbiturate + H2O strict specificity Rhodococcus erythropolis ureidomalonic acid
-
 ?
3.5.2.1 ureidomalonic acid
-
 Rhodococcus erythropolis barbiturate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.2.1 homotetramer 4 * 45000, SDS-PAGE Rhodococcus erythropolis
3.5.2.1 More partial amino acid sequence analysis Rhodococcus erythropolis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.2.1 55
-
 30 min, complete loss of activity Rhodococcus erythropolis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.2.1 8
-
 loss of activity above Rhodococcus erythropolis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.2.1 0.42
-
 cyanuric acid pH 8.0, 30°C Rhodococcus erythropolis
3.5.2.1 1.7
-
 alloxan pH 8.0, 30°C Rhodococcus erythropolis
3.5.2.1 4.5
-
 dihydro-L-orotate pH 8.0, 30°C Rhodococcus erythropolis