Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yang, H.; Zeng, Y.; Lee, T.D.; Yang, Y.; Ou, X.; Chen, L.; Haque, M.; Rippe, R.; Lu, S.C.
    Role of AP-1 in the coordinate induction of rat glutamate-cysteine ligase and glutathione synthetase by tert-butylhydroquinone (2002), J. Biol. Chem., 277, 35232-35239.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.2.2 activator protein 1 i.e. AP-1, is required for basal expression of the enzyme Rattus norvegicus
6.3.2.2 tert-butylhydroquinone i.e. TBH, exerts a dose- and time-dependent increase in the mRNA level and promotor activity of the 2 genes encoding the enzyme subunits Rattus norvegicus
6.3.2.3 activator protein 1 i.e. AP-1, mediates the induction of enzyme expression by tert-butylhydroquinone, binds to the enzyme promotor, antagonisted by c-jun, a dominant negative which is also produced in enhanced levels after treatment with tert-butylhydroquinone Rattus norvegicus
6.3.2.3 tert-butylhydroquinone regulatory role, activator protein 1 AP-1-mediated induction of enzyme expression, 35fold increase in activity in recombinant H4IIE cells, induction of c-jun formation, which is a negative regulatory protein blocking the binding of activatot protein 1 to the promotor Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.2 expression of both subunits in H4IIE cells via infection with adenovirus as a vector Rattus norvegicus
6.3.2.3 cloning of the 2.2 kb 5'-flanking region, DNA sequence determination and analysis, promotor determination, determination of positive and negative regulation regions, e.g. NF1 repressor for induction of enzyme expression by tert-butylhydroquinone, expression in H4IIE cells, ATCC CRL-1548, via transformation by recombinant adenovirus vector Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.3 c-jun negative regulation of enzyme expression by blocking the binding of AP-1 to the promotor Rattus norvegicus
6.3.2.3 tert-butylhydroquinone regulatory role, activator protein 1 AP-1-mediated induction of enzyme expression, 35fold increase in activity in recombinant H4IIE cells, induction of c-jun formation, which is a negative regulatory protein blocking the binding of activator protein 1 to the promotor Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.2 ATP + L-Glu + L-Cys Rattus norvegicus part of GSH biosynthesis ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?
6.3.2.3 ATP + gamma-L-Glu-L-Cys + Gly Rattus norvegicus
-
 ADP + phosphate + glutathione
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.2 Rattus norvegicus
-
-
-
6.3.2.3 Rattus norvegicus AF333982
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.2 ATP + L-Glu + L-Cys
-
 Rattus norvegicus ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?
6.3.2.2 ATP + L-Glu + L-Cys part of GSH biosynthesis Rattus norvegicus ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?
6.3.2.3 ATP + gamma-L-Glu-L-Cys + Gly
-
 Rattus norvegicus ADP + phosphate + glutathione
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.2.2 dimer
-
 Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
6.3.2.2 GCL
-
 Rattus norvegicus
6.3.2.3 Glutathione synthetase
-
 Rattus norvegicus
6.3.2.3 GS
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.2.2 ATP
-
 Rattus norvegicus