Literature summary extracted from

Do, H.; Lee, W.S.; Ghosh, P.; Hollowell, T.; Canfield, W.; Kornfeld, S.
Human mannose 6-phosphate-uncovering enzyme is synthesized as a proenzyme that is activated by the endoprotease furin (2002), J. Biol. Chem., 277, 29737-29744.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
3.1.4.45	furin	UCE is synthesized as an inactive proenzyme that is specifically activated by the endoprotease furin	Homo sapiens

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.1.4.45	expression in mouse L-cells, in CHO-K1 cells and in Sf9 insect cells	Homo sapiens

Protein Variants

EC Number	Protein Variants	Comment	Organism
3.1.4.45	Y488A	mutation in the 488YHPL internalization signal present in the cytoplasmic tail, mutant UCE accumulates on the cell surface	Homo sapiens

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
3.1.4.45	Golgi membrane	mainly localized to the trans-Golgi network, cycles between the network and the plasma membrane, type I transmembrane glycoprotein, containing a 24-amino acid signal sequence, a luminal domain of 423 residues, a 27-residue transmembrane region and a 41-residue cytoplasmic tail	Homo sapiens	139	-
3.1.4.45	plasma membrane	mainly localized to the trans-Golgi network and cycles between the network and plasma membrane	Homo sapiens	5886	-
3.1.4.45	soluble	recombinant UCE expressed in CHO-K1 cells and in Sf9 insect cells	Homo sapiens	-	-

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3.1.4.45	glycoprotein N-acetyl-D-glucosaminyl-phospho-D-mannose + H2O	Homo sapiens	removes a covering N-acetylglucosamine from the mannose 6-phosphate recognition marker on lysosomal acid hydrolases	N-acetyl-D-glucosamine + glycoprotein phospho-D-mannose	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.1.4.45	Homo sapiens	-	-	-

Posttranslational Modification

EC Number	Posttranslational Modification	Comment	Organism
3.1.4.45	glycoprotein	-	Homo sapiens
3.1.4.45	proteolytic modification	UCE is synthesized as an inactive proenzyme that is activated by the endoprotease furin, which is localized in the trans-Golgi network and cleaves an RARLPR/D sequence to release a 24-amino acid propiece	Homo sapiens

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.1.4.45	499fold: recombinant UCE expressed in CHO-K1 cells, 1392fold: recombinant UCE expressed in Sf9 insect cells	Homo sapiens

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
3.1.4.45	L-cell	mouse WT 1-38 L-cells expressing human wild-type UCE	Homo sapiens	-
3.1.4.45	LoVo cell	derived from a lymph node metastasis of a human colon adenocarcinoma, CCL-229, extremely low UCE activity due to a lack of the activating endoprotease furin, addition of furin restores UCE activity	Homo sapiens	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.1.4.45	4.2	-	recombinant UCE expressed in CHO-K1 cells	Homo sapiens
3.1.4.45	4.75	-	recombinant UCE expressed in Sf9 insect cells	Homo sapiens

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.1.4.45	6-O-(N-acetyl-alpha-D-glucosaminyl)phosphono-D-mannopyranose + H2O	-	Homo sapiens	N-acetyl-D-glucosamine + 6-O-phosphono-D-mannopyranose	-	?
3.1.4.45	glycoprotein N-acetyl-D-glucosaminyl-phospho-D-mannose + H2O	removes a covering N-acetylglucosamine from the mannose 6-phosphate recognition marker on lysosomal acid hydrolases	Homo sapiens	N-acetyl-D-glucosamine + glycoprotein phospho-D-mannose	-	?
3.1.4.45	methyl 6-O-(N-acetyl-alpha-D-glucosaminyl)phospho-alpha-D-mannose + H2O	-	Homo sapiens	N-acetyl-D-glucosamine + methyl alpha-D-mannose 6-phosphate	-	?
3.1.4.45	UDP-N-acetyl-D-glucosamine + H2O	-	Homo sapiens	UDP + N-acetyl-D-glucosamine	-	?

Subunits

EC Number	Subunits	Comment	Organism
3.1.4.45	tetramer	composed of two disulfide-linked homodimers	Homo sapiens

Synonyms

EC Number	Synonyms	Comment	Organism
3.1.4.45	UCE	uncovering enzyme	Homo sapiens

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Release 2023.1 (January 2023)