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Literature summary extracted from

  • Goto, M.; Nakajima, Y.; Hirotsu, K.
    Crystal structure of argininosuccinate synthetase from Thermus thermophilus HB8. Structural basis for the catalytic action (2002), J. Biol. Chem., 277, 15890-15896.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.5 overexpression in Escherichia coli Thermus thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.4.5 3-dimensional structure of free enzyme and of complexes with intact ATP, adenylyl imidodiphosphate, arginine or succinate, hanging drop method Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.5 Mg2+ essential for catalysis Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.5 44815
-
 4 * 44815, recombinant tAsS, subunit/domain structure, amino acid sequence Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.5 ATP + L-citrulline + L-aspartate Thermus thermophilus second step of urea cycle AMP + diphosphate + L-argininosuccinate
-
 ?
6.3.4.5 ATP + L-citrulline + L-aspartate Thermus thermophilus HB8 / ATCC 27634 / DSM 579 second step of urea cycle AMP + diphosphate + L-argininosuccinate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.5 Thermus thermophilus
-
-
-
6.3.4.5 Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.5
-
 Thermus thermophilus

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.4.5 ATP + L-citrulline + L-aspartate = AMP + diphosphate + 2-(Nomega-L-arginino)succinate catalytic mechanism Thermus thermophilus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.5 ATP + L-citrulline + L-aspartate ATP-dependent condensation of citrulline with aspartate, no conformational change upon binding of substrates, mechanism via citrullyl-AMP intermediate, active site structure Thermus thermophilus AMP + diphosphate + L-argininosuccinate
-
 r
6.3.4.5 ATP + L-citrulline + L-aspartate second step of urea cycle Thermus thermophilus AMP + diphosphate + L-argininosuccinate
-
 ?
6.3.4.5 ATP + L-citrulline + L-aspartate ATP-dependent condensation of citrulline with aspartate, no conformational change upon binding of substrates, mechanism via citrullyl-AMP intermediate, active site structure Thermus thermophilus HB8 / ATCC 27634 / DSM 579 AMP + diphosphate + L-argininosuccinate
-
 r
6.3.4.5 ATP + L-citrulline + L-aspartate second step of urea cycle Thermus thermophilus HB8 / ATCC 27634 / DSM 579 AMP + diphosphate + L-argininosuccinate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.5 tetramer 4 * 44815, recombinant tAsS, subunit/domain structure, amino acid sequence Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
6.3.4.5 Argininosuccinate synthetase
-
 Thermus thermophilus
6.3.4.5 ASS
-
 Thermus thermophilus
6.3.4.5 tAsS
-
 Thermus thermophilus