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Literature summary extracted from

  • Zang, T.M.; Hollman, D.A.; Crawford, P.A.; Crowder, M.W.; Makaroff, C.A.
    Arabidopsis glyoxalase II contains a zinc/iron binuclear metal center that is essential for substrate binding and catalysis (2001), J. Biol. Chem., 276, 4788-4795.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.2.6 pharmacology enzyme is a potential anti-cancer and/or anti-protozoal target, rational design of inhibitors Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.6 overexpression of wild-type and mutants in Escherichia coli BL21(DE3) Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
3.1.2.6 C140A site-directed mutagenesis, increased kcat, unaltered Km compared to the wild-type enzyme Arabidopsis thaliana
3.1.2.6 D58C site-directed mutagenesis, highly reduced kcat, unaltered Km compared to the wild-type enzyme Arabidopsis thaliana
3.1.2.6 H172R site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme Arabidopsis thaliana
3.1.2.6 H54N site-directed mutagenesis, highly reduced kcat, 2fold increased Km compared to the wild-type enzyme Arabidopsis thaliana
3.1.2.6 H59C site-directed mutagenesis, reduced activity compared to the wild-type enzyme Arabidopsis thaliana
3.1.2.6 K142A site-directed mutagenesis, 50% reduced kcat, 2fold increased Km compared to the wild-type enzyme Arabidopsis thaliana
3.1.2.6 N178A site-directed mutagenesis, reduced kcat, slightly increased Km compared to the wild-type enzyme Arabidopsis thaliana
3.1.2.6 R225A site-directed mutagenesis, increased activity compared to the wild-type enzyme Arabidopsis thaliana
3.1.2.6 R248W site-directed mutagenesis, about 8fold reduced kcat, about 10fold increased Km compared to the wild-type enzyme Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.6 additional information
-
 additional information kinetics Arabidopsis thaliana
3.1.2.6 0.06
-
 S-D-lactoylglutathione mutant R225A, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 0.062
-
 S-D-lactoylglutathione mutant C140A, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 0.063
-
 S-D-lactoylglutathione wild-type enzyme, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 0.076
-
 S-D-lactoylglutathione mutant D58C, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 0.098
-
 S-D-lactoylglutathione mutant N178A, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 0.13
-
 S-D-lactoylglutathione mutant H54N, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 0.17
-
 S-D-lactoylglutathione mutant K142A, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 0.6
-
 S-D-lactoylglutathione mutant R248W, pH 7.2, 25°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.2.6 cytosol cytosolic isozyme Arabidopsis thaliana 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.2.6 Fe2+ cytosolic isozyme, enzyme contains a zinc/iron binuclear metal center that is essential for substrate binding and catalysis, content of wild-type and mutant enzymes, overview Arabidopsis thaliana
3.1.2.6 Zn2+ cytosolic isozyme, enzyme contains a zinc/iron binuclear metal center that is essential for substrate binding and catalysis, content of wild-type and mutant enzymes, overview Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.6 additional information Arabidopsis thaliana the enzyme participates in the detoxification of cytotoxic and mutagenic 2-oxoaldehydes ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.6 Arabidopsis thaliana O24496 gene GLX2-2, cytosolic isozyme
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.2.6 S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate active site and reaction mechanism Arabidopsis thaliana
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.6 additional information
-
 activity of wild-type and mutants in crude extracts Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.6 additional information the enzyme participates in the detoxification of cytotoxic and mutagenic 2-oxoaldehydes Arabidopsis thaliana ?
-
 ?
3.1.2.6 S-D-lactoylglutathione + H2O
-
 Arabidopsis thaliana glutathione + D-lactate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.2.6 acetoacetylglutathione hydrolase
-
 Arabidopsis thaliana
3.1.2.6 Germ cell specific protein
-
 Arabidopsis thaliana
3.1.2.6 GLO II
-
 Arabidopsis thaliana
3.1.2.6 glyoxalase II
-
 Arabidopsis thaliana
3.1.2.6 hydrolase, acetoacetylglutathione
-
 Arabidopsis thaliana
3.1.2.6 hydrolase, hydroxyacylglutathione
-
 Arabidopsis thaliana
3.1.2.6 Round spermatid protein RSP29
-
 Arabidopsis thaliana
3.1.2.6 S-2-hydroxylacylglutathione hydrolase
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.2.6 10.6
-
 S-D-lactoylglutathione mutant D58C, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 10.9
-
 S-D-lactoylglutathione mutant H54N, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 484
-
 S-D-lactoylglutathione mutant R248W, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 600
-
 S-D-lactoylglutathione mutant R225A, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 1190
-
 S-D-lactoylglutathione mutant N178A, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 1760
-
 S-D-lactoylglutathione mutant K142A, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 3930
-
 S-D-lactoylglutathione wild-type enzyme, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 6780
-
 S-D-lactoylglutathione mutant C140A, pH 7.2, 25°C Arabidopsis thaliana