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Literature summary extracted from

  • Mamedov, T.G.; Suzuki, K.; Miura, K.; Kucho Ki, K.; Fukuzawa, H.
    Characteristics and sequence of phosphoglycolate phosphatase from a eukaryotic green alga Chlamydomonas reinhardtii (2001), J. Biol. Chem., 276, 45573-45579.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.18 CaCl2 the enzyme binds to Ca2+, the inhibition is recovered by addition of EGTA and MgCl2 Chlamydomonas reinhardtii
3.1.3.18 p-mercuribenzoic acid the inhibition is recovered by addition of dithiothreitol Chlamydomonas reinhardtii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.18 0.14
-
 2-Phosphoglycolate pH 8.0 Chlamydomonas reinhardtii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.18 32000
-
 2 * 32000, SDS-PAGE Chlamydomonas reinhardtii
3.1.3.18 65000
-
 gelfiltration Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.18 2-Phosphoglycolate + H2O Chlamydomonas reinhardtii a key enzyme of photorespiration in photosynthetic organisms Glycolate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.18 Aquifex aeolicus O67359
-
-
3.1.3.18 Chlamydomonas reinhardtii Q948T5 eukaryotic green alga
-
3.1.3.18 Cupriavidus necator P40852
-
-
3.1.3.18 Escherichia coli P32662
-
-
3.1.3.18 Synechocystis sp. P73525 PCC6803
-
3.1.3.18 Triticum aestivum
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.18 86.5
-
-
 Chlamydomonas reinhardtii

Storage Stability

EC Number Storage Stability Organism
3.1.3.18 -20°C, stable at least 6 months Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.18 2-Phosphoglycolate + H2O
-
 Chlamydomonas reinhardtii Glycolate + phosphate
-
 ?
3.1.3.18 2-Phosphoglycolate + H2O a key enzyme of photorespiration in photosynthetic organisms Chlamydomonas reinhardtii Glycolate + phosphate
-
 ?
3.1.3.18 D-fructose-1,6-bisphosphate + H2O
-
 Chlamydomonas reinhardtii D-fructose-1-phosphate + phosphate
-
 ?
3.1.3.18 D-ribulose-5-phosphate + H2O
-
 Chlamydomonas reinhardtii D-ribulose + phosphate
-
 ?
3.1.3.18 additional information not: 3-phosphoglycerate, D-ribose-5-phosphate, phosphoenolpyruvate, O-phospho-1-serine, p-nitrophenylphosphate Chlamydomonas reinhardtii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.18 homodimer 2 * 32000, SDS-PAGE Chlamydomonas reinhardtii

Synonyms

EC Number Synonyms Comment Organism
3.1.3.18 PGPase
-
 Chlamydomonas reinhardtii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.18 8.9 9
-
 Chlamydomonas reinhardtii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.3.18 Chlamydomonas reinhardtii isoelectric focusing
-
 5.1