EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.1 | overexpression of wild-type and mutants in Escherichia coli BL21(DE3) | Alicyclobacillus acidocaldarius |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.1.1 | M112S | site-directed and saturation mutagenesis, altered substrate specificity, higher efficiency with monoacylesters whose chain length is greater than 8 C-atoms | Alicyclobacillus acidocaldarius |
3.1.1.1 | M112S/R215L | site-directed and saturation mutagenesis, altered substrate specificity, higher efficiency with monoacylesters whose chain length is greater than 8 C-atoms | Alicyclobacillus acidocaldarius |
3.1.1.1 | M112T | site-directed and saturation mutagenesis, altered substrate specificity | Alicyclobacillus acidocaldarius |
3.1.1.1 | M112V | site-directed and saturation mutagenesis, altered substrate specificity | Alicyclobacillus acidocaldarius |
3.1.1.1 | R215L | site-directed and saturation mutagenesis, altered substrate specificity, higher efficiency with monoacylesters whose chain length is greater than 8 C-atoms | Alicyclobacillus acidocaldarius |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.1 | HEPES | inhibition mechanism, competitive | Alicyclobacillus acidocaldarius | |
3.1.1.1 | propan-2-ol | competitive | Alicyclobacillus acidocaldarius |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.1 | 0.007 | - |
4-nitrophenyl dodecanoate | mutant M211T, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 0.008 | - |
4-nitrophenyl dodecanoate | mutant M211S/R215L, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 0.008 | - |
4-nitrophenyl decanoate | mutants M211S and M211S/R215L, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 0.01 | - |
4-nitrophenyl dodecanoate | mutant M211S, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 0.011 | - |
4-nitrophenyl decanoate | mutant R215L, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 0.015 | - |
4-nitrophenyl dodecanoate | mutant M211V, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 0.017 | - |
4-nitrophenyl tetradecanoate | mutant M211S, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 0.018 | - |
4-nitrophenyl decanoate | wild-type enzyme, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 0.019 | - |
4-nitrophenyl decanoate | mutant M211T, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 0.019 | - |
4-nitrophenyl tetradecanoate | mutant M211V, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 0.019 | - |
4-nitrophenyl dodecanoate | wild-type enzyme, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 0.02 | - |
4-nitrophenyl tetradecanoate | mutant M211T, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 0.021 | - |
4-nitrophenyl hexadecanoate | mutant M211S, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 0.021 | - |
4-nitrophenyl decanoate | mutant M211V, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 0.021 | - |
4-nitrophenyl dodecanoate | mutant R215L, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 0.022 | - |
4-nitrophenyl octanoate | mutant M211S/R215L, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 0.022 | - |
4-nitrophenyl tetradecanoate | mutants R215L and M211S/R215L, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 0.023 | - |
4-nitrophenyl hexadecanoate | mutant M211T, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 0.025 | - |
4-nitrophenyl octanoate | mutant R215L, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 0.026 | - |
4-nitrophenyl hexadecanoate | mutant M211V, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 0.028 | - |
4-nitrophenyl octanoate | wild-type enzyme, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 0.031 | - |
4-nitrophenyl hexadecanoate | mutant R215L, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 0.031 | - |
4-nitrophenyl tetradecanoate | wild-type enzyme, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 0.034 | - |
4-nitrophenyl hexadecanoate | mutant M211S/R215L, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 0.037 | - |
4-nitrophenyl octanoate | mutant M211S, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 0.042 | - |
4-nitrophenyl hexadecanoate | wild-type enzyme, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 0.06 | - |
4-nitrophenyl octanoate | mutant M211V, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 0.09 | - |
4-nitrophenyl hexanoate | mutant R215L, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 0.099 | - |
4-nitrophenyl hexanoate | mutant M211S/R215L, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 0.1 | - |
4-nitrophenyl octanoate | mutant M211T, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 0.21 | - |
4-nitrophenyl hexanoate | mutant M211V, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 0.22 | - |
4-nitrophenyl hexanoate | wild-type enzyme, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 0.41 | - |
4-nitrophenyl hexanoate | mutant M211S, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 0.51 | - |
4-nitrophenyl hexanoate | mutant M211T, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 0.73 | - |
4-nitrophenyl butanoate | wild-type enzyme, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 0.75 | - |
4-nitrophenyl butanoate | mutant R215L, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 1.06 | - |
4-nitrophenyl butanoate | mutant M211S/R215L, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 1.27 | - |
4-nitrophenyl butanoate | mutant M211V, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 1.77 | - |
4-nitrophenyl butanoate | mutant M211S, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 1.86 | - |
4-nitrophenyl butanoate | mutant M211T, pH 7.1, 70°C | Alicyclobacillus acidocaldarius |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.1 | Alicyclobacillus acidocaldarius | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.1 | recombinant wild-type and mutants from Escherichia coli BL21(DE3) | Alicyclobacillus acidocaldarius |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.1.1.1 | a carboxylic ester + H2O = an alcohol + a carboxylate | reaction mechanism | Alicyclobacillus acidocaldarius |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.1 | 4-nitrophenyl acetate + H2O | - |
Alicyclobacillus acidocaldarius | 4-nitrophenol + acetate | - |
r | |
3.1.1.1 | 4-nitrophenyl butanoate + H2O | best substrate of mutants M211V, M211T, M211S and M211S/R215L | Alicyclobacillus acidocaldarius | 4-nitrophenol + butanoate | - |
r | |
3.1.1.1 | 4-nitrophenyl decanoate + H2O | - |
Alicyclobacillus acidocaldarius | 4-nitrophenol + decanoate | - |
r | |
3.1.1.1 | 4-nitrophenyl dodecanoate + H2O | - |
Alicyclobacillus acidocaldarius | 4-nitrophenol + dodecanoate | - |
r | |
3.1.1.1 | 4-nitrophenyl hexadecanoate + H2O | - |
Alicyclobacillus acidocaldarius | 4-nitrophenol + hexadecanoate | - |
r | |
3.1.1.1 | 4-nitrophenyl hexanoate + H2O | best substrate of the wild-type enzyme | Alicyclobacillus acidocaldarius | 4-nitrophenol + hexanoate | - |
r | |
3.1.1.1 | 4-nitrophenyl octanoate + H2O | - |
Alicyclobacillus acidocaldarius | 4-nitrophenol + octanoate | - |
r | |
3.1.1.1 | 4-nitrophenyl tetradecanoate + H2O | - |
Alicyclobacillus acidocaldarius | 4-nitrophenol + tetradecanoate | - |
r | |
3.1.1.1 | additional information | substrate specificities of wild-type and mutant enzymes, reverse reaction in performed in organic solvents | Alicyclobacillus acidocaldarius | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.1 | EST2 | - |
Alicyclobacillus acidocaldarius |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.1 | 70 | - |
- |
Alicyclobacillus acidocaldarius |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.1 | 7 | - |
4-nitrophenyl hexadecanoate | mutant M211V, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 8.3 | - |
4-nitrophenyl hexadecanoate | mutant M211T, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 15.6 | - |
4-nitrophenyl hexadecanoate | mutant M211S, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 21 | - |
4-nitrophenyl hexadecanoate | wild-type enzyme, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 27 | - |
4-nitrophenyl tetradecanoate | mutant M211V, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 35 | - |
4-nitrophenyl hexadecanoate | mutant R215L, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 40 | - |
4-nitrophenyl tetradecanoate | mutant M211T, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 44 | - |
4-nitrophenyl hexadecanoate | mutant M211S/R215L, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 105 | - |
4-nitrophenyl tetradecanoate | wild-type enzyme and mutant M211S, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 110 | - |
4-nitrophenyl dodecanoate | mutant M211T, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 112 | - |
4-nitrophenyl dodecanoate | mutant M211V, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 232 | - |
4-nitrophenyl tetradecanoate | mutant R215L, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 235 | - |
4-nitrophenyl tetradecanoate | mutant M211S/R215L, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 350 | - |
4-nitrophenyl dodecanoate | mutant M211S, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 390 | - |
4-nitrophenyl dodecanoate | wild-type enzyme, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 465 | - |
4-nitrophenyl decanoate | mutant M211V, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 626 | - |
4-nitrophenyl decanoate | mutant M211T, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 705 | - |
4-nitrophenyl dodecanoate | mutant R215L, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 860 | - |
4-nitrophenyl decanoate | mutant M211S, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 940 | - |
4-nitrophenyl dodecanoate | mutant M211S/R215L, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 950 | - |
4-nitrophenyl decanoate | wild-type enzyme, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 970 | - |
4-nitrophenyl decanoate | mutant M211S/R215L, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 1376 | - |
4-nitrophenyl octanoate | mutant M211V, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 1380 | - |
4-nitrophenyl octanoate | mutant M211V, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 1500 | - |
4-nitrophenyl hexanoate | mutant M211V, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 1645 | - |
4-nitrophenyl octanoate | mutant M211S/R215L, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 1650 | - |
4-nitrophenyl octanoate | mutant M211S/R215L, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 1670 | - |
4-nitrophenyl decanoate | mutant R215L, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 1740 | - |
4-nitrophenyl octanoate | mutant M211T, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 1855 | - |
4-nitrophenyl octanoate | mutant M211S, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 1860 | - |
4-nitrophenyl octanoate | mutant M211S, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 1960 | - |
4-nitrophenyl octanoate | wild-type enzyme, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 2180 | - |
4-nitrophenyl butanoate | wild-type enzyme, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 2300 | - |
4-nitrophenyl hexanoate | mutant M211S/R215L, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 2750 | - |
4-nitrophenyl butanoate | mutant M211S/R215L, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 3080 | - |
4-nitrophenyl octanoate | mutant R215L, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 3200 | - |
4-nitrophenyl butanoate | mutant R215L, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 3420 | - |
4-nitrophenyl hexanoate | wild-type enzyme, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 3660 | - |
4-nitrophenyl hexanoate | mutant R215L, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 3890 | - |
4-nitrophenyl hexanoate | mutant M211S, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 4000 | - |
4-nitrophenyl butanoate | mutant M211V, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 4230 | - |
4-nitrophenyl butanoate | mutant M211S, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 4440 | - |
4-nitrophenyl hexanoate | mutant M211T, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 5240 | - |
4-nitrophenyl butanoate | mutant M211T, pH 7.1, 70°C | Alicyclobacillus acidocaldarius |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.1 | 7.1 | - |
- |
Alicyclobacillus acidocaldarius |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.1 | 130 | - |
propan-2-ol | competitive, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
3.1.1.1 | 780 | - |
HEPES | competitive, pH 7.1, 70°C | Alicyclobacillus acidocaldarius |