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Literature summary extracted from

  • Fitzpatrick, T.B.; Killer, P.; Thomas, R.M.; Jelesarov, I.; Amrhein, N.; Macheroux, P.
    Chorismate synthase from the hyperthermophile Thermotoga maritima combines thermostability and increased rigidity with catalytic and spectral properties similar to mesophilic counterparts (2001), J. Biol. Chem., 276, 18052-18059.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.5 expression in Escherichia coli Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.5 41754
-
 4 * 41754, calculation from amino acid sequence Thermotoga maritima
4.2.3.5 42000
-
 4 * 42000, SDS-PAGE Thermotoga maritima
4.2.3.5 200000
-
 non-denaturing PAGE in presence of O5-(1-carboxyvinyl)-3-phosphoshikimate and FMN Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.5 O5-(1-carboxyvinyl)-3-phosphoshikimate Thermotoga maritima enzyme of the shikimate pathway chorismate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.5 Thermotoga maritima Q9WYI2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.5 recombinant enzyme Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.5 additional information monofunctional enzyme that does not have an intrinsic ability to reduce the FMN cofactor Thermotoga maritima ?
-
 ?
4.2.3.5 O5-(1-carboxyvinyl)-3-phosphoshikimate
-
 Thermotoga maritima chorismate + phosphate
-
 ?
4.2.3.5 O5-(1-carboxyvinyl)-3-phosphoshikimate enzyme of the shikimate pathway Thermotoga maritima chorismate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.3.5 tetramer 4 * 42000, SDS-PAGE Thermotoga maritima
4.2.3.5 tetramer 4 * 41754, calculation from amino acid sequence Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.3.5 92
-
 melting temperature above Thermotoga maritima

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.3.5 FMN cofactor Thermotoga maritima