Literature summary extracted from
Marabotti, A.; De Biase, D.; Tramonti, A.; Bettati, S.; Mozzarelli, A.
Allosteric communication of tryptophan synthase. Functional and regulatory properties of the beta S178P mutant (2001), J. Biol. Chem., 276, 17747-17753.
Application
EC Number |
Application |
Comment |
Organism |
---|
4.2.1.20 |
analysis |
the enzyme complex is a model enzyme for understanding allosteric regulation |
Salmonella enterica subsp. enterica serovar Typhimurium |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
4.2.1.20 |
expressionof mutant S178P in Escherichia coli |
Salmonella enterica subsp. enterica serovar Typhimurium |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
4.2.1.20 |
hanging drop method, 10 mg/ml purified recombinant mutant S178P enzyme in 50 mM bicine, pH 7.8, with equal volume of reservoir solution: 50 mM bicine, 1 mM EDTA, 12% w/v PEG 8000, 1.4 mM spermine, pH 7.8, 21°C, structure determination and functional investigation by polarized absorption microspectrophotometry |
Salmonella enterica subsp. enterica serovar Typhimurium |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
4.2.1.20 |
S178P |
site-directed mutagenesis, molecular modeling of the structural effects caused by the mutation, hydrogen bond with alphaGlu181 is interrupted, 2fold decreased activity of the beta-subunit, not affected, in contrast to the wild-type, by allosteric effectors indole-3-acetylglycine and DL-alpha-glycerol-3-phosphate in the beta-reaction, weaker binding of Na+ |
Salmonella enterica subsp. enterica serovar Typhimurium |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.2.1.20 |
Salmonella enterica subsp. enterica serovar Typhimurium |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
4.2.1.20 |
recombinant mutant S178P from Escherichia coli |
Salmonella enterica subsp. enterica serovar Typhimurium |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
4.2.1.20 |
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O |
also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8) |
Salmonella enterica subsp. enterica serovar Typhimurium |
|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.2.1.20 |
1-(indol-3-yl)glycerol 3-phosphate |
alpha-subunit of the bienzyme complex, alpha-reaction |
Salmonella enterica subsp. enterica serovar Typhimurium |
D-glyceraldehyde 3-phosphate + indole |
- |
? |
|
4.2.1.20 |
L-serine + indole |
beta-subunit of the bienzyme complex, beta-reaction |
Salmonella enterica subsp. enterica serovar Typhimurium |
L-tryptophan + H2O |
- |
? |
|
4.2.1.20 |
additional information |
allosteric communication, Ser178 is a critical residue in ligand-triggered signals between alpha and beta active sites |
Salmonella enterica subsp. enterica serovar Typhimurium |
? |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
4.2.1.20 |
tetramer |
alpha2beta2 complex |
Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
4.2.1.20 |
pyridoxal 5'-phosphate |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
|