Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Duchene, A.M.; Peeters, N.; Dietrich, A.; Cosset, A.; Small, I.D.; Wintz, H.
    Overlapping destinations for two dual targeted glycyl-tRNA synthetases in Arabidopsis thaliana and Phaseolus vulgaris (2001), J. Biol. Chem., 276, 15275-15283.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.14 overexpression of isozyme GlyRS1 in Escherichia coli strain TG2 Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.14 chloroplast isozyme GlyRS2 Phaseolus vulgaris 9507
-
6.1.1.14 chloroplast isozyme GlyRS2 Arabidopsis thaliana 9507
-
6.1.1.14 cytosol isozyme GlyRS1, active Phaseolus vulgaris 5829
-
6.1.1.14 cytosol isozyme GlyRS1, active Arabidopsis thaliana 5829
-
6.1.1.14 mitochondrion isozyme GlyRS2, enzyme is imported from the cytosol, nucleus-encoded Arabidopsis thaliana 5739
-
6.1.1.14 mitochondrion isozymes GlyRS1 and GlyRS2, enzymes are imported from the cytosol, nucleus-encoded, isozyme GlyRS1 is inactive in the mitochondria Phaseolus vulgaris 5739
-
6.1.1.14 mitochondrion isozymes GlyRS1, enzyme is imported from the cytosol, nucleus-encoded, isozyme GlyRS1 is inactive in the mitochondria Arabidopsis thaliana 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.14 27000
-
 2-3 * 27000, about, partially processed isozyme GlyRS1, SDS-PAGE Arabidopsis thaliana
6.1.1.14 30000
-
 4 * 30000, about, partially processed isozyme GlyRS2, SDS-PAGE Arabidopsis thaliana
6.1.1.14 80000
-
 isozyme GlyRS1 Arabidopsis thaliana
6.1.1.14 117000
-
 isozyme GlyRS2 Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.14 ATP + glycine + tRNAGly Phaseolus vulgaris
-
 AMP + diphosphate + glycyl-tRNAGly
-
 ?
6.1.1.14 ATP + glycine + tRNAGly Arabidopsis thaliana
-
 AMP + diphosphate + glycyl-tRNAGly
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.14 Arabidopsis thaliana O23627 2 dual-targeted isozymes
-
6.1.1.14 Arabidopsis thaliana Q8L785 2 dual-targeted isozymes
-
6.1.1.14 Phaseolus vulgaris
-
 2 dual-targeted isozymes
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.14 recombinant isozyme GlyRS1 from Escherichia coli Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.14 ATP + glycine + tRNAGly
-
 Phaseolus vulgaris AMP + diphosphate + glycyl-tRNAGly
-
 ?
6.1.1.14 ATP + glycine + tRNAGly
-
 Arabidopsis thaliana AMP + diphosphate + glycyl-tRNAGly
-
 ?
6.1.1.14 ATP + glycine + tRNAGly chloroplastic and mitochondrial tRNA substrate specificity of chloroplastic isozyme GlyRS2, isozyme GlyRS1 is much less efficient Phaseolus vulgaris AMP + diphosphate + glycyl-tRNAGly
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.14 dimer or trimer 2-3 * 27000, about, partially processed isozyme GlyRS1, SDS-PAGE Arabidopsis thaliana
6.1.1.14 tetramer 4 * 30000, about, partially processed isozyme GlyRS2, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
6.1.1.14 Glycine--tRNA ligase
-
 Phaseolus vulgaris
6.1.1.14 Glycine--tRNA ligase
-
 Arabidopsis thaliana
6.1.1.14 Glycyl translase
-
 Phaseolus vulgaris
6.1.1.14 Glycyl translase
-
 Arabidopsis thaliana
6.1.1.14 Glycyl-transfer ribonucleate synthetase
-
 Phaseolus vulgaris
6.1.1.14 Glycyl-transfer ribonucleate synthetase
-
 Arabidopsis thaliana
6.1.1.14 Glycyl-transfer ribonucleic acid synthetase
-
 Phaseolus vulgaris
6.1.1.14 Glycyl-transfer ribonucleic acid synthetase
-
 Arabidopsis thaliana
6.1.1.14 Glycyl-transfer RNA synthetase
-
 Phaseolus vulgaris
6.1.1.14 Glycyl-transfer RNA synthetase
-
 Arabidopsis thaliana
6.1.1.14 Glycyl-tRNA synthetase
-
 Phaseolus vulgaris
6.1.1.14 Glycyl-tRNA synthetase
-
 Arabidopsis thaliana
6.1.1.14 GlyRS
-
 Phaseolus vulgaris
6.1.1.14 GlyRS
-
 Arabidopsis thaliana
6.1.1.14 Synthetase, glycyl-transfer ribonucleate
-
 Phaseolus vulgaris
6.1.1.14 Synthetase, glycyl-transfer ribonucleate
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.14 ATP
-
 Phaseolus vulgaris
6.1.1.14 ATP
-
 Arabidopsis thaliana