EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.4.37 | wild-type and mutant CNP1, expression in Escherichia coli BL21 | Rattus norvegicus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.4.37 | C231A | CNP1 catalytic fragment mutant, kinetics | Rattus norvegicus |
3.1.4.37 | C231S | CNP1 catalytic fragment mutant, kinetics | Rattus norvegicus |
3.1.4.37 | C236A | CNP1 catalytic fragment mutant, kinetics | Rattus norvegicus |
3.1.4.37 | C236S | CNP1 catalytic fragment mutant, kinetics | Rattus norvegicus |
3.1.4.37 | C314A | CNP1 catalytic fragment mutant, kinetics | Rattus norvegicus |
3.1.4.37 | C314S | CNP1 catalytic fragment mutant, kinetics | Rattus norvegicus |
3.1.4.37 | C397S | CNP1 catalytic fragment mutant, kinetics | Rattus norvegicus |
3.1.4.37 | H230F | almost inactive CNP1 catalytic fragment mutant with 1000fold lower kcat-value and similar Km-value compared with wild-type CNP1 | Rattus norvegicus |
3.1.4.37 | H230L | almost inactive CNP1 catalytic fragment mutant with 1000fold lower kcat-value and similar Km-value compared with wild-type CNP1 | Rattus norvegicus |
3.1.4.37 | H309F | almost inactive CNP1 catalytic fragment mutant with 1000fold lower kcat-value and similar Km-value compared with wild-type CNP1 | Rattus norvegicus |
3.1.4.37 | H309L | almost inactive CNP1 catalytic fragment mutant with 1000fold lower kcat-value and similar Km-value compared with wild-type CNP1 | Rattus norvegicus |
3.1.4.37 | additional information | CNP1: N-terminal deletion mutants, catalytic fragment corresponding to the last C-terminal 250 amino acids | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.37 | 2'-AMP | product inhibitor | Rattus norvegicus | |
3.1.4.37 | 5,5'-dithiobis-(2-nitrobenzoic acid) | 97% inhibition of the catalytic fragment of CNP1 in a time- and dose-dependent manner, kinetics, fully reversed by excess dithiothreitol or 2-mercaptoethanol, inhibition is attributable to steric effects of modification of Cys-236 and Cys-314 by the inhibitor | Rattus norvegicus | |
3.1.4.37 | diethylpyrocarbonate | pH 6.5, 22°C, time-dependent inhibition, kinetics, completely reversed by 0.5 M hydroxylamine | Rattus norvegicus | |
3.1.4.37 | KCN | 62% inhibition of the catalytic fragment of CNP1 | Rattus norvegicus | |
3.1.4.37 | methyl methanethiosulfonate | 42% inhibition of the catalytic fragment of CNP1 | Rattus norvegicus | |
3.1.4.37 | additional information | not inhibited by dithiothreitol | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.4.37 | 0.098 | - |
2',3'-cNADP | pH 6, 25°C, H309L mutant CNP1 | Rattus norvegicus | |
3.1.4.37 | 0.1 | - |
2',3'-cNADP | pH 6, 25°C, H309F mutant CNP1 | Rattus norvegicus | |
3.1.4.37 | 0.104 | - |
2',3'-cNADP | pH 6, 25°C, H230L mutant CNP1 | Rattus norvegicus | |
3.1.4.37 | 0.119 | - |
2',3'-cNADP | pH 6, 25°C, H230F mutant CNP1 | Rattus norvegicus | |
3.1.4.37 | 0.231 | - |
2',3'-cNADP | pH 6, 25°C, C231A mutant CNP1 | Rattus norvegicus | |
3.1.4.37 | 0.237 | - |
2',3'-cNADP | pH 6, 25°C, recombinant full-length CNP1 | Rattus norvegicus | |
3.1.4.37 | 0.241 | - |
2',3'-cNADP | pH 6, 25°C, C314S mutant CNP1 | Rattus norvegicus | |
3.1.4.37 | 0.295 | - |
2',3'-cNADP | pH 6, 25°C, catalytic fragment of CNP1 corresponding to the C-terminal 250 amino acids | Rattus norvegicus | |
3.1.4.37 | 0.333 | - |
2',3'-cNADP | pH 6, 25°C, C314A mutant CNP1 | Rattus norvegicus | |
3.1.4.37 | 0.354 | - |
2',3'-cNADP | pH 6, 25°C, C236A mutant CNP1 | Rattus norvegicus | |
3.1.4.37 | 0.379 | - |
2',3'-cNADP | pH 6, 25°C, C236S mutant CNP1 | Rattus norvegicus | |
3.1.4.37 | 0.473 | - |
2',3'-cNADP | pH 6, 25°C, C231S mutant CNP1 | Rattus norvegicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.4.37 | 46000 | - |
x * 46000, CNP1, x * 48000, CNP2 | Rattus norvegicus |
3.1.4.37 | 48000 | - |
x * 46000, CNP1, x * 48000, CNP2 | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.37 | additional information | Rattus norvegicus | CNP is one of the earliest myelin-related proteins to be expressed in differentiated oligodendrocytes and Schwann cells, role in migration and/or expansion of membranes during myelination | ? | - |
? |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
3.1.4.37 | Ethanol | at a final concentration up to 4% v/v of the total volume, no effect on CNP1 activity | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.4.37 | Rattus norvegicus | - |
2 isoforms: CNP1 and CNP2 differing by a 20-amino acid extension at the N-terminus | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.1.4.37 | side-chain modification | isoprenylated at Cys-397 | Rattus norvegicus |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.4.37 | recombinant wild-type and mutant CNP1 | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.37 | 2',3'-cNADP+ + H2O | - |
Rattus norvegicus | NADP+ | - |
? | |
3.1.4.37 | 2',3'-cyclic NADP+ + H2O | - |
Rattus norvegicus | NADP+ | - |
? | |
3.1.4.37 | additional information | CNP is one of the earliest myelin-related proteins to be expressed in differentiated oligodendrocytes and Schwann cells, role in migration and/or expansion of membranes during myelination | Rattus norvegicus | ? | - |
? | |
3.1.4.37 | nucleoside 2',3'-cyclic phosphate + H2O | His-230 and His-309 of CNP1 play critical roles in enzyme catalysis, no essential role of cysteines, the catalytic domain forms a compact globular structure | Rattus norvegicus | nucleoside 2'-phosphate | - |
ir |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.4.37 | ? | x * 46000, CNP1, x * 48000, CNP2 | Rattus norvegicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.4.37 | 25 | - |
assay at | Rattus norvegicus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.4.37 | 1.15 | - |
2',3'-cNADP | pH 6, 25°C, H230F mutant CNP1 | Rattus norvegicus | |
3.1.4.37 | 1.16 | - |
2',3'-cNADP | pH 6, 25°C, H230L mutant CNP1 | Rattus norvegicus | |
3.1.4.37 | 1.27 | - |
2',3'-cNADP | pH 6, 25°C, H309L mutant CNP1 | Rattus norvegicus | |
3.1.4.37 | 1.35 | - |
2',3'-cNADP | pH 6, 25°C, H309F mutant CNP1 | Rattus norvegicus | |
3.1.4.37 | 6.08 | - |
2',3'-cNADP | pH 6, 25°C, H230L mutant CNP1 | Rattus norvegicus | |
3.1.4.37 | 6.08 | - |
2',3'-cNADP | pH 6, 25°C, H309L mutant CNP1 | Rattus norvegicus | |
3.1.4.37 | 594 | - |
2',3'-cNADP | pH 6, 25°C, C314S mutant CNP1 | Rattus norvegicus | |
3.1.4.37 | 825 | - |
2',3'-cNADP | pH 6, 25°C, C231S mutant CNP1 | Rattus norvegicus | |
3.1.4.37 | 968 | - |
2',3'-cNADP | pH 6, 25°C, C231A mutant CNP1 | Rattus norvegicus | |
3.1.4.37 | 1107 | - |
2',3'-cNADP | pH 6, 25°C, C236S mutant CNP1 | Rattus norvegicus | |
3.1.4.37 | 1110 | - |
2',3'-cNADP | pH 6, 25°C, C236S mutant CNP1 | Rattus norvegicus | |
3.1.4.37 | 1116 | - |
2',3'-cNADP | pH 6, 25°C, C314A mutant CNP1 | Rattus norvegicus | |
3.1.4.37 | 1120 | - |
2',3'-cNADP | pH 6, 25°C, C314A mutant CNP1 | Rattus norvegicus | |
3.1.4.37 | 1130 | - |
2',3'-cNADP | pH 6, 25°C, C397S mutant CNP1 | Rattus norvegicus | |
3.1.4.37 | 1132 | - |
2',3'-cNADP | pH 6, 25°C, C397S mutant CNP1 | Rattus norvegicus | |
3.1.4.37 | 1195 | - |
2',3'-cNADP | pH 6, 25°C, recombinant full-length wild-type CNP1 | Rattus norvegicus | |
3.1.4.37 | 1200 | - |
2',3'-cNADP | pH 6, 25°C, recombinant full-length wild-type CNP1 | Rattus norvegicus | |
3.1.4.37 | 1476 | - |
2',3'-cNADP | pH 6, 25°C, C236A mutant CNP1 | Rattus norvegicus | |
3.1.4.37 | 1480 | - |
2',3'-cNADP | pH 6, 25°C, C236A mutant CNP1 | Rattus norvegicus | |
3.1.4.37 | 1690 | - |
2',3'-cNADP | pH 6, 25°C, catalytic fragment of CNP1 corresponding to the C-terminal 250 amino acids | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.4.37 | 6 | - |
assay at | Rattus norvegicus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.4.37 | 0.5 | - |
2'-AMP | - |
Rattus norvegicus |