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Literature summary extracted from

  • Fujino, T.; Kondo, J.; Ishikawa, M.; Morikawa, K.; Yamamoto, T.T.
    Acetyl-CoA synthetase 2, a mitochondrial matrix enzyme involved in the oxidation of acetate (2001), J. Biol. Chem., 276, 11420-11426.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.2.1.1 cytosol AceCS1 Mus musculus 5829
-
6.2.1.1 mitochondrion matrix, AceCS2 Mus musculus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.1 71000
-
 x * 71000, AceCS2, SDS-PAGE Mus musculus
6.2.1.1 74309
-
 x * 74309, calculation from nucleotide sequence Bos taurus
6.2.1.1 74662
-
 x * 74662, AceCS2, calculation from nucleotide sequence Mus musculus
6.2.1.1 78000
-
 x * 78000, AceCS1, SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.1 dATP + acetate + CoA Mus musculus AceCS2 plays a role in the production of energy under ketogenic conditions, such as starvation and diabetes. Acetyl-CoAs produced by AceCS2 are utilized mainly for oxidation dAMP + diphosphate + acetyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.1 Bos taurus Q9BEA3
-
-
6.2.1.1 Mus musculus
-
-
-
6.2.1.1 Mus musculus Q99NB1
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
6.2.1.1 proteolytic modification the putative mitochondrial targeting signal is cleaved during the transportation of the enzyme into the mitochondria matrix Mus musculus

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.1
-
 Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.1 3T3-L1 cell marked induction of AceCS1 mRNA and protein during differentiation of 3T3-L1 cells, neither AceCS2 mRNA nor protein is detected in undifferentiated or differentiated 3T3-L1 cells Mus musculus
-
6.2.1.1 brain AceCS2, low activity Mus musculus
-
6.2.1.1 heart AceCS2, high activity Mus musculus
-
6.2.1.1 kidney AceCS2, high activity Mus musculus
-
6.2.1.1 liver AceCS1, no AceCS2 activity Mus musculus
-
6.2.1.1 lung AceCS2, high activity Mus musculus
-
6.2.1.1 additional information no AceCS2 activity is detected in liver. Marked induction of AceCS1 mRNA and protein during differentiation of 3T3-L1 cells, neither AceCS2 mRNA nor protein is detected in undifferentiated or differentiated 3T3-L1 cells Mus musculus
-
6.2.1.1 skeletal muscle AceCS2, high activity Mus musculus
-
6.2.1.1 spleen AceCS2, high activity Mus musculus
-
6.2.1.1 testis AceCS2, high activity Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.1 11.9
-
 AceCS2 Mus musculus
6.2.1.1 26.8
-
 AceCS1 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.1 dATP + acetate + CoA
-
 Mus musculus dAMP + diphosphate + acetyl-CoA
-
 ?
6.2.1.1 dATP + acetate + CoA AceCS2 plays a role in the production of energy under ketogenic conditions, such as starvation and diabetes. Acetyl-CoAs produced by AceCS2 are utilized mainly for oxidation Mus musculus dAMP + diphosphate + acetyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.1 ? x * 71000, AceCS2, SDS-PAGE Mus musculus
6.2.1.1 ? x * 74309, calculation from nucleotide sequence Bos taurus
6.2.1.1 ? x * 74662, AceCS2, calculation from nucleotide sequence Mus musculus
6.2.1.1 ? x * 78000, AceCS1, SDS-PAGE Mus musculus

Synonyms

EC Number Synonyms Comment Organism
6.2.1.1 AceCS1 liver enzyme, 45.8% amino acid identity between AceCS1 and AceCS2 Mus musculus
6.2.1.1 AceCS2 heart enzyme, 45.8% amino acid identity between AceCS1 and AceCS2 Mus musculus