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Literature summary extracted from
- CyaG, a novel cyanobacterial adenylyl cyclase and a possible ancestor of mammalian guanylyl cyclases (2001), J. Biol. Chem., 276, 10564-10569.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.6.1.1 | expression of C-terminal domain as GST-fusion protein in Escherichia coli | Arthrospira platensis |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 4.6.1.1 | K533E | no activity | Arthrospira platensis |
| 4.6.1.1 | K533E/I603R/D605C | mutant lose adenylyl cyclase activity but obtains significant guanylyl cyclase activity | Arthrospira platensis |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 4.6.1.1 | membrane | - |
Arthrospira platensis | 16020 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.6.1.1 | Mn2+ | 1 mM, strong activation | Arthrospira platensis |  |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.6.1.1 | ATP | Arthrospira platensis | - |
3',5'-cyclic AMP + diphosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.6.1.1 | Arthrospira platensis | Q9EXQ2 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.6.1.1 | recombinant C-terminal domain | Arthrospira platensis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.6.1.1 | 0.00036 | - |
in the presence of 1 mM Mg2+, recombinant C-terminal CyaG domain | Arthrospira platensis |
| 4.6.1.1 | 0.011 | - |
in the presence of 1 mM Mn2+, recombinant C-terminal CyaG domain | Arthrospira platensis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.6.1.1 | ATP | - |
Arthrospira platensis | 3',5'-cyclic AMP + diphosphate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.6.1.1 | adenylyl cyclase | - |
Arthrospira platensis |
| 4.6.1.1 | CyaG | - |
Arthrospira platensis |
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Release 2023.1 (January 2023)
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