Literature summary extracted from
Furuya, T.; Kashuba, C.; Docampo, R.; Moreno, S.N.J.
A novel phosphatidylinositol-phospholipase C of Trypanosoma cruzi that is lipid modified and activated during trypomastigote to amastigote differentiation (2000), J. Biol. Chem., 275, 6428-6438.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
3.1.4.11 |
additional information |
TcPI-PLC expression is induced during the differentiation of trypomastigotes into amastigotes |
Trypanosoma cruzi |
|
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.1.4.11 |
expression in Escherichia coli BL21(DE3), sequencing |
Trypanosoma cruzi |
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
3.1.4.11 |
0.023 |
- |
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate |
pH 7.1, 30°C |
Trypanosoma cruzi |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
3.1.4.11 |
plasma membrane |
associated with, in amastigotes |
Trypanosoma cruzi |
5886 |
- |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.1.4.11 |
Ca2+ |
dependent on free Ca2+, enzyme needs a higher calcium concentration for its activation with phosphatidylinositol as substrate than with phosphatidylinositol 4,5-bisphosphate |
Trypanosoma cruzi |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.1.4.11 |
80000 |
- |
x * 82000, calculated from the amino acid sequence, x * 80000, SDS-PAGE, Western blot analysis |
Trypanosoma cruzi |
3.1.4.11 |
82000 |
- |
x * 82000, calculated from the amino acid sequence, x * 80000, SDS-PAGE, Western blot analysis |
Trypanosoma cruzi |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
3.1.4.11 |
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O |
Trypanosoma cruzi |
inositol phosphate/diacylglycerol signaling pathway, TcPI-PLC is lipid modified in vivo, TcPI-PLC is developmentally regulated, its expression is induced during the differentiation of trypomastigotes into amastigotes at an early stage in the transformation process |
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.1.4.11 |
Trypanosoma cruzi |
Q9TZN8 |
- |
- |
Posttranslational Modification
EC Number |
Posttranslational Modification |
Comment |
Organism |
---|
3.1.4.11 |
lipoprotein |
TcPI-PLC is lipid modified in vivo, TcPI-PLC contains an N-myristoylation consensus sequence at its N-terminal end and is modified by myristate and palmitate, myristoyl group may play an important role in the association of enzyme with the plasma membrane |
Trypanosoma cruzi |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.1.4.11 |
recombinant TcPI-PLC |
Trypanosoma cruzi |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
3.1.4.11 |
amastigote |
TcPI-PLC expression at high levels in the epimastigote and amastigote stages |
Trypanosoma cruzi |
- |
3.1.4.11 |
epimastigote |
TcPI-PLC expression at high levels in the epimastigote and amastigote stages |
Trypanosoma cruzi |
- |
3.1.4.11 |
trypomastigote |
lower TcPI-PLC expression than in epimastigotes and amastigotes |
Trypanosoma cruzi |
- |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
3.1.4.11 |
additional information |
- |
- |
Trypanosoma cruzi |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.1.4.11 |
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O |
phosphoinositide-specific, preferred substrate to phosphatidylinositol, domain structure |
Trypanosoma cruzi |
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol |
- |
? |
|
3.1.4.11 |
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O |
inositol phosphate/diacylglycerol signaling pathway, TcPI-PLC is lipid modified in vivo, TcPI-PLC is developmentally regulated, its expression is induced during the differentiation of trypomastigotes into amastigotes at an early stage in the transformation process |
Trypanosoma cruzi |
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol |
- |
? |
|
3.1.4.11 |
phosphatidylinositol + H2O |
phosphatidylinositol 4,5-bisphosphate is preferred to phosphatidylinositol |
Trypanosoma cruzi |
myo-inositol 1-phosphate + diacylglycerol |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.1.4.11 |
? |
x * 82000, calculated from the amino acid sequence, x * 80000, SDS-PAGE, Western blot analysis |
Trypanosoma cruzi |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.1.4.11 |
TcPI-PLC |
PI-PLC of Trypanosoma cruzi, similar to mamalian delta-type PI-PLCs |
Trypanosoma cruzi |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.1.4.11 |
30 |
- |
assay at |
Trypanosoma cruzi |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.1.4.11 |
6.7 |
6.8 |
- |
Trypanosoma cruzi |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
3.1.4.11 |
6.5 |
7.3 |
enzyme activity decreases very rapidly below pH 6.5 and above pH 7.25 |
Trypanosoma cruzi |