EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.2.1.20 | purified mutant A169L/C170W complexed with the alpha-active site substrate analogue 5-fluoro-indole-propanol-phosphate, X-ray diffraction structure determination and analysis at 2.25 A resolution | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.1.20 | A169L/C170W | mutants of beta-subunit residues, altered kinetic properties and conformation | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.20 | additional information | - |
additional information | kinetics of wild-type enzyme complex and mutant betaA169L/betaC170W | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.20 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
4.2.1.20 | L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O | also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8), mechanism | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.20 | 1-(indol-3-yl)glycerol 3-phosphate | alpha-subunit of the bifunctional enzyme complex, alpha-reaction | Salmonella enterica subsp. enterica serovar Typhimurium | D-glyceraldehyde 3-phosphate + indole | - |
? | |
4.2.1.20 | L-serine + indole | beta-subunit of the bifunctional enzyme complex, beta-reaction | Salmonella enterica subsp. enterica serovar Typhimurium | L-tryptophan + H2O | - |
? | |
4.2.1.20 | additional information | substrate channeling, Thr170 occludes the tunnel connecting the alpha- and beta-active sites, accumulation of the intermediate indole during a single enzyme turnover | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.1.20 | tetramer | alpha2beta2 complex, structural organization | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.1.20 | TRPS | - |
Salmonella enterica subsp. enterica serovar Typhimurium |