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Literature summary extracted from

  • Tolkunova, E.; Park, H.; Xia, J.; King, M.P.; Davidson, E.
    The human lysyl-tRNA synthetase gene encodes both the cytoplasmic and mitochondrial enzymes by means of an unusual alternative splicing of the primary transcript (2000), J. Biol. Chem., 275, 35063-35069.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.6 DNA sequence determination and analysis, genomic organization, expression of cytoplasmic isozyme in Escherichia coli strain BL21(DE3) as His-tagged enzymes, expression in human osteosarcoma cell line 143B Homo sapiens
6.1.1.6 DNA sequence determination and analysis, genomic organization, expression of mitochondrial isozyme in Escherichia coli strain BL21(DE3) as His-tagged enzymes, expression in human osteosarcoma cell line 143B Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.6 cytoplasm cytoplasmic isozyme Homo sapiens 5737
-
6.1.1.6 mitochondrion mitochondrial isozyme Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.6 ATP + L-lysine + tRNALys Homo sapiens
-
 AMP + L-lysyl-tRNALys + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.6 Homo sapiens Q15046 1 gene encodes a cytoplasmic isozyme, the second encodes both a cytoplasmic and mitochondrial isozyme, which are differenciated by alternative splicing
-
6.1.1.6 Homo sapiens Q15046 1 gene encodes a cytoplasmic isozyme, the second encodes both a cytoplasmic and mitochondrial isozyme, which are differentiated by alternative splicing
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.6 0.00002
-
 purified recombinant isozyme, mitochondrial tRNALys substrate Homo sapiens
6.1.1.6 0.0001
-
 purified recombinant isozyme, mitochondrial tRNALys substrate Homo sapiens
6.1.1.6 0.0032
-
 purified recombinant isozyme, cytoplasmic tRNALys substrate Homo sapiens
6.1.1.6 0.0133
-
 purified recombinant isozyme, cytoplasmic tRNALys substrate Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.6 ATP + L-lysine + tRNALys
-
 Homo sapiens AMP + L-lysyl-tRNALys + diphosphate
-
 ?
6.1.1.6 ATP + L-lysine + tRNALys isozyme shows different activities with cytoplasmic and mitochondrial tRNALys Homo sapiens AMP + L-lysyl-tRNALys + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.6 L-Lysine-transfer RNA ligase
-
 Homo sapiens
6.1.1.6 Lysine translase
-
 Homo sapiens
6.1.1.6 Lysine--tRNA ligase
-
 Homo sapiens
6.1.1.6 Lysine-tRNA synthetase
-
 Homo sapiens
6.1.1.6 LysRS
-
 Homo sapiens
6.1.1.6 Lysyl-transfer ribonucleate synthetase
-
 Homo sapiens
6.1.1.6 Lysyl-transfer RNA synthetase
-
 Homo sapiens
6.1.1.6 Lysyl-tRNA synthetase
-
 Homo sapiens
6.1.1.6 Synthetase, lysyl-transfer ribonucleate
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.6 37
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.6 ATP
-
 Homo sapiens