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Literature summary extracted from

  • Deng, S.J.; Bickett, D.M.; Mitchell, J.L.; Lambert, M.H.; Blackburn, R.K.; Carter, H.L., 3rd; Neugebauer, J.; Pahel, G.; Weiner, M.P.; Moss, M.L.
    Substrate specificity of human collagenase 3 assessed using a phage-displayed peptide library (2000), J. Biol. Chem., 275, 31422-31427.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.B4 overexpression in Escherichia coli BL21(DE3) Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.B4 matrix metalloprotease inhibitor GW9471 broad spectrum Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.B4 protein + H2O Homo sapiens
-
 peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.B4 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.B4 recombinant refolded enzyme form Escherichia coli Homo sapiens

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.4.24.B4 recombinant enzyme form inclusion bodies after expression in Escherichia coli Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.B4 additional information
-
 substrate specificity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.B4 2,4-dinitrophenyl-GPLGMRGL-NH2 + H2O
-
 Homo sapiens ?
-
 ?
3.4.24.B4 aggrecan + H2O
-
 Homo sapiens ?
-
 ?
3.4.24.B4 alpha2-chain of type IV collagen + H2O cleavage sites at amino acid residues 881 and 1000 in the triple-helical region Homo sapiens ?
-
 ?
3.4.24.B4 alpha4-chain of type IV collagen + H2O cleavage site at amino acid residue 86 in the triple-helical region Homo sapiens ?
-
 ?
3.4.24.B4 protein + H2O
-
 Homo sapiens peptides
-
 ?
3.4.24.B4 protein + H2O substrate specificity and cleavage site determination via phage display approach Homo sapiens peptides
-
 ?
3.4.24.B4 Transforming growth factor + H2O cleavage site at amino acid residue 150 in the latency-associated peptide Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.B4 M10.013 Merops-ID Homo sapiens
3.4.24.B4 matrix metalloproteinase-13
-
 Homo sapiens
3.4.24.B4 MMP-13
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.24.B4 0.0000005
-
 matrix metalloprotease inhibitor GW9471 37°C, recombinant enzyme Homo sapiens