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Literature summary extracted from

  • Pinzar, E.; Miyano, M.; Kanaoka, Y.; Urade, Y.; Hayaishi, O.
    Structural basis of hematopoietic prostaglandin D synthase activity elucidated by site-directed mutagenesis (2000), J. Biol. Chem., 275, 31239-31244.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.99.2
-
Rattus norvegicus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.99.2
-
Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
5.3.99.2 C156L loss of prostaglandin D synthase activity, retention of glutathione S-transferase activity Rattus norvegicus
5.3.99.2 C156Y loss of prostaglandin D synthase activity, retention of glutathione S-transferase activity Rattus norvegicus
5.3.99.2 K112E retention of prostaglandin D synthase and glutathione S-transferase activity Rattus norvegicus
5.3.99.2 K198E retention of prostaglandin D synthase and glutathione S-transferase activity Rattus norvegicus
5.3.99.2 L199F retention of prostaglandin D synthase and glutathione S-transferase activity Rattus norvegicus
5.3.99.2 R14E complete loss of prostaglandin D synthase activity and glutathione S-transferase activity Rattus norvegicus
5.3.99.2 R14K complete loss of prostaglandin D synthase activity and glutathione S-transferase activity Rattus norvegicus
5.3.99.2 W104I complete loss of prostaglandin D synthase activity and glutathione S-transferase activity Rattus norvegicus
5.3.99.2 Y152F retention of prostaglandin D synthase and glutathione S-transferase activity Rattus norvegicus
5.3.99.2 Y8F complete loss of prostaglandin D synthase activity and glutathione S-transferase activity Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.99.2 0.1
-
glutathione
-
Rattus norvegicus
5.3.99.2 0.5
-
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
Rattus norvegicus
5.3.99.2 0.5
-
glutathione conjugation of glutathione to 1-chloro-2,4-dinitrobenzene Rattus norvegicus
5.3.99.2 3
-
1-chloro-2,4-dinitrobenzene conjugation of glutathione to 1-chloro-2,4-dinitrobenzene Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.99.2 (5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate + 2 GSH Rattus norvegicus
-
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + GSSG + 2 H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
5.3.99.2 Rattus norvegicus O35543
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.99.2 30
-
-
Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.99.2 (5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
Rattus norvegicus (5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate
-
?
5.3.99.2 (5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate + 2 GSH
-
Rattus norvegicus (5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + GSSG + 2 H+
-
?
5.3.99.2 glutathione + 1-chloro-2,4-dinitrobenzene
-
Rattus norvegicus ?
-
?

Synonyms

EC Number Synonyms Comment Organism
5.3.99.2 Hematopoietic prostaglandin D synthase i.e. PGDS Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.99.2 2
-
glutathione conjugation of glutathione to 1-chloro-2,4-dinitrobenzene Rattus norvegicus
5.3.99.2 5
-
1-chloro-2,4-dinitrobenzene conjugation of glutathione to 1-chloro-2,4-dinitrobenzene Rattus norvegicus
5.3.99.2 15
-
glutathione
-
Rattus norvegicus
5.3.99.2 21.7
-
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
5.3.99.2 glutathione
-
Rattus norvegicus